LOCUS       sequence-313445-        6139 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Localization: Membrane, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6139)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-CD36-C-10
FEATURES             Location/Qualifiers
     source          1..6139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        99..402
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        403..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     557..577
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      645..654
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             651..1358
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(796..814)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1064..1083
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2965..3086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3002..3021)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3056..3075
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3093..3548)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3230..3251)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3442..3461
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3575..3679
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3681..4038
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3681..3872
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3704..3724)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3889..4024
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4073..4867
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4073..4867
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4127..4146)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4737..4756
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5055..5074)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5099..5146
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5475..6063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5964..5983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt
       61 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      121 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      181 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      241 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      301 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      361 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      421 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      481 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      541 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      601 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc atggtgagca
      661 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      721 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      781 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      841 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      901 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      961 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     1021 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     1081 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1141 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1201 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1261 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1321 gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga tctggcagca
     1381 gtggaggtgg ctgtgaccgg aactgtgggc tcatcgctgg ggctgtcatt ggtgctgtcc
     1441 tggctgtgtt tggaggtatt ctaatgccag ttggagacct gcttatccag aagacaatta
     1501 aaaagcaagt tgtcctcgaa gaaggtacaa ttgcttttaa aaattgggtt aaaacaggca
     1561 cagaagttta cagacagttt tggatctttg atgtgcaaaa tccacaggaa gtgatgatga
     1621 acagcagcaa cattcaagtt aagcaaagag gtccttatac gtacagagtt cgttttctag
     1681 ccaaggaaaa tgtaacccag gacgctgagg acaacacagt ctctttcctg cagcccaatg
     1741 gtgccatctt cgaaccttca ctatcagttg gaacagaggc tgacaacttc acagttctca
     1801 atctggctgt ggcagctgca tcccatatct atcaaaatca atttgttcaa atgatcctca
     1861 attcacttat taacaagtca aaatcttcta tgttccaagt cagaactttg agagaactgt
     1921 tatggggcta tagggatcca tttttgagtt tggttccgta ccctgttact accacagttg
     1981 gtctgtttta tccttacaac aatactgcag atggagttta taaagttttc aatggaaaag
     2041 ataacataag taaagttgcc ataatcgaca catataaagg taaaaggaat ctgtcctatt
     2101 gggaaagtca ctgcgacatg attaatggta cagatgcagc ctcatttcca ccttttgttg
     2161 agaaaagcca ggtattgcag ttcttttctt ctgatatttg caggtcaatc tatgctgtat
     2221 ttgaatccga cgttaatctg aaaggaatcc ctgtgtatag atttgttctt ccatccaagg
     2281 cctttgcctc tccagttgaa aacccagaca actattgttt ctgcacagaa aaaattatct
     2341 caaaaaattg tacatcatat ggtgtgctag acatcagcaa atgcaaagaa gggagacctg
     2401 tgtacatttc acttcctcat tttctgtatg caagtcctga tgtttcagaa cctattgatg
     2461 gattaaaccc aaatgaagaa gaacatagga catacttgga tattgaacct ataactggat
     2521 tcactttaca atttgcaaaa cggctgcagg tcaacctatt ggtcaagcca tcagaaaaaa
     2581 ttcaagtatt aaagaatctg aagaggaact atattgtgcc tattctttgg cttaatgaga
     2641 ctgggaccat tggtgatgag aaggcaaaca tgttcagaag tcaagtaact ggaaaaataa
     2701 acctccttgg cctgatagaa atgatcttac tcagtgttgg tgtggtgatg tttgttgctt
     2761 ttatgatttc atattgtgca tgcagatcga aaacaataaa ataagaattc tgcagtcgac
     2821 ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag ccataccaca
     2881 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     2941 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     3001 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3061 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa
     3121 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3181 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     3241 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     3301 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     3361 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     3421 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     3481 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     3541 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3601 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3661 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     3721 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3781 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3841 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3901 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3961 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4021 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     4081 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4141 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4201 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     4261 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4321 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4381 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4441 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     4501 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     4561 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     4621 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     4681 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     4741 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     4801 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     4861 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     4921 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     4981 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     5041 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     5101 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     5161 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     5221 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     5281 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     5341 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5401 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5461 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5521 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5581 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5641 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5701 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5761 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5821 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5881 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5941 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6001 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6061 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6121 atgttctttc ctgcgttat
//
