LOCUS       sequence-313499-       10306 bp    DNA     circular SYN 14-APR-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, PGK 
            promoter, Hygro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 10306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti PGK Hygro DEST (w530-1)
FEATURES             Location/Qualifiers
     source          1..10306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     203..222
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             288..1313
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             288..1313
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             1687..1920
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1992..2113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2029..2048)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2083..2102
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2153..2288
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2308..2327)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2309..2327)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2337..2354)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2337..2353)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2346..2368)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2495..2950
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2582..2601)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2792..2813
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2976..3080
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3081..3149,3150..3941)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3299..3318)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4112..4700
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4601..4620
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4854..4871
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4988..5009
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5024..5029,5030..5047,5048..5054)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5062..5078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5067..5089
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5086..5102
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5086..5102
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5121..5141
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5123..5141
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5169..5395
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5396..5576
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5623..5748
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6241..6474
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6659..6703
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6970..7087
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7133..7643
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7562..7580
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    7698..7822
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7847..7852,7853..7870,7871..7877)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7931..8590
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7997..8016)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8932..9237
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9116..9135
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(9278..9402)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    9433..10021
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9486..9506)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9904..9915)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(10077..10306,1..270)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10112..10135)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
       61 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
      121 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
      181 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
      241 ttctcctctt cctcatctcc gggcctttcg acctgcatcc cgccaccatg aaaaagcctg
      301 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
      361 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
      421 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
      481 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
      541 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
      601 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
      661 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
      721 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
      781 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
      841 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
      901 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
      961 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
     1021 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
     1081 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
     1141 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
     1201 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
     1261 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagagtagat
     1321 gccgaccgaa caagagctga tttcgagaac gcctcagcca gcaactcgcg cgagcctagc
     1381 aaggcaaatg cgagagaacg gccttacgct tggtggcaca gttctcgtcc acagttcgct
     1441 aagctcgctc ggctgggtcg cgggagggcc ggtcgcagtg attcaggccc ttctggattg
     1501 tgttggtccc cagggcacga ttgtcatgcc cacgcactcg ggtgatctga ctgatcccgc
     1561 agattggaga tcgccgcccg tgcctgccga ttgggtgcag atccgtcgag ggcccggtac
     1621 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     1681 ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg
     1741 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     1801 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     1861 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     1921 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     1981 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     2041 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     2101 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     2161 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2221 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     2281 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     2341 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     2401 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     2461 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     2521 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2581 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2641 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2701 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2761 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     2821 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     2881 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     2941 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3001 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3061 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3121 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3181 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3241 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     3301 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     3361 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     3421 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     3481 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     3541 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     3601 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     3661 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     3721 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3781 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     3841 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     3901 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     3961 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4021 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4081 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4141 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4201 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4261 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4321 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4381 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4441 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4501 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4561 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4621 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4681 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4741 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4801 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4861 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     4921 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     4981 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     5041 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     5101 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     5161 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     5221 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     5281 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     5341 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     5401 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5461 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5521 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     5581 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     5641 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     5701 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     5761 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     5821 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     5881 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     5941 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     6001 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     6061 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     6121 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     6181 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     6241 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     6301 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     6361 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     6421 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     6481 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     6541 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     6601 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     6661 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     6721 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     6781 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     6841 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     6901 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     6961 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     7021 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     7081 aaattttatc gatcacgaga ctagcctcga gaagcttgat atcgaattcc acggggttgg
     7141 ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
     7201 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
     7261 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
     7321 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
     7381 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
     7441 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
     7501 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
     7561 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc
     7621 gaatcaccga cctctctccc cagggggatc caccggttcg tcgactagtc cagtgtggtg
     7681 gaattctgca gatatcaaca agtttgtaca aaaaagctga acgagaaacg taaaatgata
     7741 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
     7801 cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc
     7861 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa
     7921 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     7981 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     8041 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     8101 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     8161 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     8221 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     8281 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     8341 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     8401 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     8461 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     8521 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     8581 cggggcgtaa agatctggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
     8641 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
     8701 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
     8761 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
     8821 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
     8881 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
     8941 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     9001 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
     9061 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     9121 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     9181 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
     9241 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt
     9301 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     9361 atcattttac gtttctcgtt cagctttctt gtacaaagtg gttgatatcc agcacagtgg
     9421 cggccgctcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     9481 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     9541 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     9601 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     9661 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     9721 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     9781 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     9841 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     9901 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     9961 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
    10021 cctggaattc tgcagatatc cggactagtg atctctcgag gttaacgaat tctaccgggt
    10081 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
    10141 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
    10201 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
    10261 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatg
//
