LOCUS       sequence-313569-        9189 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9189)
  AUTHORS   Rosenbluh J, Nijhawan D, Cox AG, Li X, Neal JT, Schafer EJ, Zack TI,
            Wang X, Tsherniak A, Schinzel AC, Shao DD, Schumacher SE, Weir BA, 
            Vazquez F, Cowley GS, Root DE, Mesirov JP, Beroukhim R, Kuo CJ, 
            Goessling W, Hahn WC
  TITLE     beta-Catenin-Driven Cancers Require a YAP1 Transcriptional Complex 
            for Survival and Tumorigenesis.
  JOURNAL   Cell. 2012 Dec 21;151(7):1457-73. doi: 10.1016/j.cell.2012.11.026. 
            Epub 2012 Dec 13.
  PUBMED    23245941
REFERENCE   2  (bases 1 to 9189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.11.026"; journalName: "Cell"; date: 
            "2012-12-21- 21"; volume: "151"; issue: "7"; pages: "1457-73"
COMMENT     Sequence Label: YAP1 (S6A) - V5 in pLX304
FEATURES             Location/Qualifiers
     source          1..9189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2023..2047)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2049..2090
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2132..2720
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2185..2205)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2603..2614)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2792..3025
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3103..3224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3140..3159)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3194..3213
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3264..3399
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3419..3438)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3420..3438)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3448..3465)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3448..3464)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3457..3479)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3606..4061
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3693..3712)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3903..3924
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4087..4191
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4192..4260,4261..5052)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4410..4429)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5223..5811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5712..5731
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5965..5982
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6099..6120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6135..6140,6141..6158,6159..6165)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6173..6189
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6178..6200
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6197..6213
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6197..6213
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6232..6252
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6234..6252
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6280..6506
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6507..6687
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6734..6859
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7352..7585
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7770..7814
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7989..8489
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8408..8426
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8501..8896
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8956..9073
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagt tggcatggat cccgggcagc agccgccgcc tcaaccggcc ccccagggcc
      601 aagggcagcc gccttcgcag cccccgcagg ggcagggccc gccgtccgga cccgggcaac
      661 cggcacccgc ggcgacccag gcggcgccgc aggcaccccc cgccgggcat cagatcgtgc
      721 acgtccgcgg ggacgcggag accgacctgg aggcgctctt caacgccgtc atgaacccca
      781 agacggccaa cgtgccccag accgtgccca tgaggctccg gaagctgccc gactccttct
      841 tcaagccgcc ggagcccaaa tcccactccc gacaggccgc tactgatgca ggcactgcag
      901 gagccctgac tccacagcat gttcgagctc atgccgctcc agctgctctg cagttgggag
      961 ctgtttctcc tgggacactg acccccactg gagtagtctc tggcccagca gctacaccca
     1021 cagctcagca tcttcgacag gctgcttttg agatacctga tgatgtacct ctgccagcag
     1081 gttgggagat ggcaaagaca tcttctggtc agagatactt cttaaatcac atcgatcaga
     1141 caacaacatg gcaggacccc aggaaggcca tgctgtccca gatgaacgtc acagccccca
     1201 ccagtccacc agtgcagcag aatatgatga actcggcttc aggtcctctt cctgatggat
     1261 gggaacaagc catgactcag gatggagaaa tttactatat aaaccataag aacaagacca
     1321 cctcttggct agacccaagg cttgaccctc gttttgccat gaaccagaga atcagtcaga
     1381 gtgctccagt gaaacagcca ccacccctgg ctccccagag cccacaggga ggcgtcatgg
     1441 gtggcagcaa ctccaaccag cagcaacaga tgcgactgca gcaactgcag atggagaagg
     1501 agaggctgcg gctgaaacag caagaactgc ttcggcagga gttagccctg cgtagccagt
     1561 taccaacact ggagcaggat ggtgggactc aaaatccagt gtcttctccc gggatgtctc
     1621 aggaattgag aacaatgacg accaatagct cagatccttt ccttaacagt ggcacctatc
     1681 actctcgaga tgaggctaca gacagtggac taagcatgag cagctacagt gtccctcgaa
     1741 ccccagatga cttcctgaac agtgtggatg agatggatac aggtgatact atcaaccaaa
     1801 gcaccctgcc ctcacagcag aaccgtttcc cagactacct tgaagccatt cctgggacaa
     1861 atgtggacct tggaacactg gaaggagatg gaatgaacat agaaggagag gagctgatgc
     1921 caagtctgca ggaagctttg agttctgaca tccttaatga catggagtct gttttggctg
     1981 ccaccaagct agataaagaa agctttctta catggttacc acccccaact ttcttgtaca
     2041 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
     2101 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggattaca aaatttgtga
     2161 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     2221 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     2281 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     2341 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     2401 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     2461 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     2521 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     2581 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     2641 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     2701 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat
     2761 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     2821 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     2881 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     2941 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     3001 tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag
     3061 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     3121 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3181 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     3241 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3301 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     3361 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     3421 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     3481 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     3541 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     3601 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3661 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3721 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3781 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     3841 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     3901 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     3961 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4021 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     4081 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4141 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4201 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4261 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4321 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4381 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4441 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4501 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4561 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4621 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4681 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4741 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4801 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4861 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4921 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4981 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5041 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5101 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5161 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5221 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5281 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5341 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5401 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5461 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5521 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5581 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5641 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5701 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5761 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5821 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5881 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     5941 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     6001 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     6061 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     6121 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     6181 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     6241 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     6301 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     6361 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     6421 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     6481 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     6541 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6601 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6661 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     6721 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     6781 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     6841 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     6901 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     6961 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     7021 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     7081 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     7141 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     7201 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     7261 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     7321 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     7381 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     7441 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     7501 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     7561 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     7621 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     7681 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     7741 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     7801 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     7861 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     7921 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     7981 ggggaccctt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg
     8041 ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg
     8101 ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg
     8161 ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg
     8221 aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc
     8281 gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg
     8341 gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc
     8401 ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg
     8461 ttgaccgaat caccgacctc tctccccagg gggtaccacc atggccaagc ctttgtctca
     8521 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
     8581 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
     8641 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
     8701 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
     8761 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
     8821 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
     8881 ttatgtgtgg gagggcctgc agctgcagta gtaagaattc tagatcttga gacaaatggc
     8941 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     9001 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     9061 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga
     9121 gggggcccgg ggttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     9181 ggagttccg
//
