LOCUS       sequence-313735-        8913 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Bacterial expression of Tn5-APEX2 fusion protein with linker 
            (GGGGS)4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Lee JD, Paulo JA, Posey RR, Mugoni V, Kong NR, Cheloni G, Lee YR, 
            Slack FJ, Tenen DG, Clohessy JG, Gygi SP, Pandolfi PP
  TITLE     Dual DNA and protein tagging of open chromatin unveils dynamics of 
            epigenomic landscapes in leukemia.
  JOURNAL   Nat Methods. 2021 Mar;18(3):293-302. doi: 
            10.1038/s41592-021-01077-8. Epub 2021 Mar 1.
  PUBMED    33649590
REFERENCE   2  (bases 1 to 8913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-021-01077-8"; journalName: "Nat Methods"; date: 
            "2021-03"; volume: "18"; issue: "3"; pages: "293-302"
COMMENT     Sequence Label: pTXB1-TP4
FEATURES             Location/Qualifiers
     source          1..8913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..78)
                     /gene="lacI"
                     /label=lacI promoter
     terminator      231..317
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      414..500
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      597..683
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      780..866
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      963..1049
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1184..1203
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1184..1202
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1203..1227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1271..2698
                     /codon_start=1
                     /product="transposase from the bacterial Tn5 transposon 
                     (Reznikoff, 1993)"
                     /label=Tn5 transposase
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     CDS             2759..3505
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             3506..3529
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3530..4123
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             4154..4309
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     primer_bind     complement(4374..4392)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4388..4435
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4495..4599
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4600..4668,4669..5460)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4818..4837)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(5502..6015)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5697..5718)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5909..5928
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6126..6714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6615..6634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6868..6885
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6907..6926)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7067..7089
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7083..7274)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7831..8913)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8875..8894
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
       61 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga
      121 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgcc
      181 ggcatgccgc cctttcgtct tcaagaatta attcccaatt ccccaggcat caaataaaac
      241 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      301 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
      361 ggtggcgggc aggacgcccg ccataaactg ccaggaatta attccccagg catcaaataa
      421 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      481 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg
      541 gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa ttaattcccc aggcatcaaa
      601 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      661 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
      721 ccggagggtg gcgggcagga cgcccgccat aaactgccag gaattaattc cccaggcatc
      781 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      841 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac
      901 ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggaattaa ttccccaggc
      961 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1021 cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc
     1081 aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggaat tggggatcgg
     1141 aattaattcc cggtttaaac cggggatctc gatcccgcga aattaatacg actcactata
     1201 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgttaac tttaagaagg
     1261 agatatacat atgattacca gtgcactgca tcgtgcggcg gattgggcga aaagcgtgtt
     1321 ttctagtgct gcgctgggtg atccgcgtcg taccgcgcgt ctggtgaatg ttgcggcgca
     1381 actggccaaa tatagcggca aaagcattac cattagcagc gaaggcagca aagccatgca
     1441 ggaaggcgcg tatcgtttta ttcgtaatcc gaacgtgagc gcggaagcga ttcgtaaagc
     1501 gggtgccatg cagaccgtga aactggccca ggaatttccg gaactgctgg caattgaaga
     1561 taccacctct ctgagctatc gtcatcaggt ggcggaagaa ctgggcaaac tgggtagcat
     1621 tcaggataaa agccgtggtt ggtgggtgca tagcgtgctg ctgctggaag cgaccacctt
     1681 tcgtaccgtg ggcctgctgc atcaagaatg gtggatgcgt ccggatgatc cggcggatgc
     1741 ggatgaaaaa gaaagcggca aatggctggc cgctgctgca acttcgcgtc tgagaatggg
     1801 cagcatgatg agcaacgtga ttgcggtgtg cgatcgtgaa gcggatattc atgcgtatct
     1861 gcaagataaa ctggcccata acgaacgttt tgtggtgcgt agcaaacatc cgcgtaaaga
     1921 tgtggaaagc ggcctgtatc tgtatgatca cctgaaaaac cagccggaac tgggcggcta
     1981 tcagattagc attccgcaga aaggcgtggt ggataaacgt ggcaaacgta aaaaccgtcc
     2041 ggcgcgtaaa gcgagcctga gcctgcgtag cggccgtatt accctgaaac agggcaacat
     2101 taccctgaac gcggtgctgg ccgaagaaat taatccgccg aaaggcgaaa ccccgctgaa
     2161 atggctgctg ctgaccagcg agccggtgga aagtctggcc caagcgctgc gtgtgattga
     2221 tatttatacc catcgttggc gcattgaaga atttcacaaa gcgtggaaaa cgggtgcggg
     2281 tgcggaacgt cagcgtatgg aagaaccgga taacctggaa cgtatggtga gcattctgag
     2341 ctttgtggcg gtgcgtctgc tgcaactgcg tgaatctttt actccgccgc aagcactgcg
     2401 tgcgcagggc ctgctgaaag aagcggaaca cgttgaaagc cagagcgcgg aaaccgtgct
     2461 gaccccggat gaatgccaac tgctgggcta tctggataaa ggcaaacgca aacgcaaaga
     2521 aaaagcgggc agcctgcaat gggcgtatat ggcgattgcg cgtctgggcg gctttatgga
     2581 tagcaaacgt accggcattg cgagctgggg tgcgctgtgg gaaggttggg aagcgctgca
     2641 aagcaaactg gatggctttc tggccgcgaa agacctgatg gcgcagggca ttaaaatcgg
     2701 cggaggtggt tctggcggtg gaggttcagg cggtggtgga agtggcggag gtggttcagg
     2761 aaagtcttac ccaactgtga gtgctgatta ccaggacgcc gttgagaagg cgaagaagaa
     2821 gctcagaggc ttcatcgctg agaagagatg cgctcctcta atgctccgtt tggcattcca
     2881 ctctgctgga acctttgaca agggcacgaa gaccggtgga cccttcggaa ccatcaagca
     2941 ccctgccgaa ctggctcaca gcgctaacaa cggtcttgac atcgctgtta ggcttttgga
     3001 gccactcaag gcggagttcc ctattttgag ctacgccgat ttctaccagt tggctggcgt
     3061 tgttgccgtt gaggtcacgg gtggacctaa ggttccattc caccctggaa gagaggacaa
     3121 gcctgagcca ccaccagagg gtcgcttgcc cgatcccact aagggttctg accatttgag
     3181 agatgtgttt ggcaaagcta tggggcttac tgaccaagat atcgttgctc tatctggggg
     3241 tcacactatt ggagctgcac acaaggagcg ttctggattt gagggtccct ggacctctaa
     3301 tcctcttatt ttcgacaact catacttcac ggagttgttg agtggtgaga aggaaggtct
     3361 ccttcagcta ccttctgaca aggctctttt gtctgaccct gtattccgcc ctctcgttga
     3421 caaatatgca gcggacgaag atgccttctt tgctgattac gctgaggctc accaaaagct
     3481 ttccgagctt gggtttgctg atgccgatta caaggatgac gacgataagt gcatcacggg
     3541 agatgcacta gttgccctac ccgagggcga gtcggtacgc atcgccgaca tcgtgccggg
     3601 tgcgcggccc aacagtgaca acgccatcga cctgaaagtc cttgaccggc atggcaatcc
     3661 cgtgctcgcc gaccggctgt tccactccgg cgagcatccg gtgtacacgg tgcgtacggt
     3721 cgaaggtctg cgtgtgacgg gcaccgcgaa ccacccgttg ttgtgtttgg tcgacgtcgc
     3781 cggggtgccg accctgctgt ggaagctgat cgacgaaatc aagccgggcg attacgcggt
     3841 gattcaacgc agcgcattca gcgtcgactg tgcaggtttt gcccgcggga aacccgaatt
     3901 tgcgcccaca acctacacag tcggcgtccc tggactggtg cgtttcttgg aagcacacca
     3961 ccgagacccg gacgcccaag ctatcgccga cgagctgacc gacgggcggt tctactacgc
     4021 gaaagtcgcc agtgtcaccg acgccggcgt gcagccggtg tatagccttc gtgtcgacac
     4081 ggcagaccac gcgtttatca cgaacgggtt cgtcagccac gctactggcc tcaccggtct
     4141 gaactcaggc ctcacgacaa atcctggtgt atccgcttgg caggtcaaca cagcttatac
     4201 tgcgggacaa ttggtcacat ataacggcaa gacgtataaa tgtttgcagc cccacacctc
     4261 cttggcagga tgggaaccat ccaacgttcc tgccttgtgg cagcttcaat gactgcagga
     4321 aggggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     4381 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
     4441 aggaggaact atatccggat aactacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     4501 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4561 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4621 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4681 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4741 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt cccaatgatg
     4801 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     4861 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4921 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4981 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5041 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5101 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5161 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5221 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5281 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5341 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5401 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5461 ctgtcagacc aagtttactc atatatactt tagattgatt taccccggtt gataatcaga
     5521 aaagccccaa aaacaggaag attgtataag caaatattta aattgtaaac gttaatattt
     5581 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     5641 tcggcaaaat cccttataaa tcaaaagaat agcccgagat agggttgagt gttgttccag
     5701 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     5761 tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt ttggggtcga
     5821 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     5881 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     5941 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     6001 cgctataggg cgcgtaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6061 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6121 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6181 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6241 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     6301 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6361 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6421 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6481 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6541 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6601 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6661 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6721 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6781 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6841 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc tatggtgcac
     6901 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     6961 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     7021 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     7081 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     7141 gcgtggtcgt gcagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     7201 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     7261 tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata
     7321 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta
     7381 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc
     7441 actcagggtc aatgccagcc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg
     7501 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct
     7561 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc
     7621 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt
     7681 tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
     7741 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
     7801 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7861 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7921 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
     7981 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
     8041 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
     8101 cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
     8161 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
     8221 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
     8281 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
     8341 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
     8401 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
     8461 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
     8521 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
     8581 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
     8641 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
     8701 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
     8761 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     8821 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     8881 ctctgcgaca tcgtataacg ttactggttt cac
//
