LOCUS       sequence-313805-        1143 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses AGSC domain from Acetobacter type I SMODS/CD-NTase enzyme.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1143)
  TITLE     Bacterial expression plasmids for production of recombinant AGSC 
            domains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGEX4-4T-1 Acetobacter AGSC
FEATURES             Location/Qualifiers
     source          1..1143
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..22
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             23..43
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             458..475
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(538..560)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(705..723)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        791..895
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggntctggtt ccgcgtggat ccgagaatct ttacttccaa ggtaataaga cgagttacat
       61 ggaagtcccg cgctggcaca gagctccatc aacacagcaa ctgtccgtga gcatccgggc
      121 ggagttgtac caacacaagt ttagcacgac accgcttggg ttcgtaaact ccttggaacc
      181 gctgcaatct gggttttttc tgcagtttaa ggctataatg aacagtggca cgcccttctc
      241 atcagccttg tacaaagttt attggaggat cacgaacacc gacgaagcag ccgcagccga
      301 aaactgcctc aggggcaaca tcgaggagcc agagtccgac aactcccgat gggagcaact
      361 gcgctataga ggggttcata tagtggaggc tttcgtcgta aataagcgga ctaaccaaat
      421 tgtgggacag tctgaagcat tccgcgtgat gattgaacat caccaccacc atcactagcg
      481 gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
      541 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
      601 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
      661 gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg tgatacgcct
      721 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
      781 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      841 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      901 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      961 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgann
     1021 nnntacatcg aactggntct cacagcggta agatccttga nanttttcgc cccganangt
     1081 tttcnngatg ancantttna ngtcngctat gngngcnnnt tatccnngnt gacncngnan
     1141 anc
//
