LOCUS       sequence-313845-        9340 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Allows for constituitive mammalian expression of the SARS-CoV 3CL 
            protease with C145A mutation via transfection or lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Resnick SJ, Iketani S, Hong SJ, Zask A, Liu H, Kim S, Melore S, Lin 
            FY, Nair MS, Huang Y, Lee S, Tay NES, Rovis T, Yang HW, Xing L, 
            Stockwell BR, Ho DD, Chavez A
  TITLE     Inhibitors of Coronavirus 3CL Proteases Protect Cells from 
            Protease-Mediated Cytotoxicity.
  JOURNAL   J Virol. 2021 Jun 24;95(14):e0237420. doi: 10.1128/JVI.02374-20. 
            Epub 2021 Jun 24.
  PUBMED    33910954
REFERENCE   2  (bases 1 to 9340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2021-06-24"; volume: "95(14):e0237420. doi"; pages: " 
            10.1128/JVI.02374-20. Epub 2021 Jun 24"
COMMENT     Sequence Label: pLEX307-SARS-CoV-3CL-C145A
FEATURES             Location/Qualifiers
     source          1..9340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2249..2273)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2281..2322
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2373..2961
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2426..2446)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2844..2855)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3033..3266
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3344..3465
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3381..3400)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3435..3454
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3505..3640
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3660..3679)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3661..3679)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3689..3706)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3689..3705)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3698..3720)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3847..4302
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3934..3953)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4144..4165
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4328..4432
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4433..4501,4502..5293)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4651..4670)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5464..6052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5953..5972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6206..6223
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6340..6361
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6376..6381,6382..6399,6400..6406)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6414..6430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6419..6441
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6438..6454
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6438..6454
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6473..6493
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6475..6493
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6521..6747
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6748..6928
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6975..7100
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7593..7826
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8011..8055
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8286..8496
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8308..8504
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8355..8490
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8536..9138
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8536..8555)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9032..9052
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9181..9298
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac
     1321 aaaatgagtg gttttaggaa aatggcattc ccgtcaggca aagttgaagg gtgcatggta
     1381 caagtaacct gtggaactac aactcttaat ggattgtggt tggatgacac agtatactgt
     1441 ccaagacatg tcatttgcac agcagaagac atgcttaatc ctaactatga agatctgctc
     1501 attcgcaaat ccaaccatag ctttcttgtt caggctggca atgttcaact tcgtgttatt
     1561 ggccattcta tgcaaaattg tctgcttagg cttaaagttg atacttctaa ccctaagaca
     1621 cccaagtata aatttgtccg tatccaacct ggtcaaacat tttcagttct agcatgctac
     1681 aatggttcac catctggtgt ttatcagtgt gccatgagac ctaatcatac cattaaaggt
     1741 tctttcctta atggatcagc cggtagtgtt ggttttaaca ttgattatga ttgcgtgtct
     1801 ttctgctata tgcatcatat ggagcttcca acaggagtac acgctggtac tgacttagaa
     1861 ggtaaattct atggtccatt tgttgacaga caaactgcac aggctgcagg tacagacaca
     1921 accataacat taaatgtttt ggcatggctg tatgctgctg ttatcaatgg tgataggtgg
     1981 tttcttaata gattcaccac tactttgaat gactttaacc ttgtggcaat gaagtacaac
     2041 tatgaacctt tgacacaaga tcatgttgac atattgggac ctctttctgc tcaaacagga
     2101 attgccgtct tagatatgtg tgctgctttg aaagagctgc tgcagaatgg tatgaatggt
     2161 cgtactatcc ttggtagcac tattttagaa gatgagttta caccatttga tgttgttaga
     2221 caatgctctg gtgttacctt ccaataaaac ccagctttct tgtacaaagt ggttgatatc
     2281 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtacc
     2341 ggttaagtcg acaatcaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     2401 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     2461 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     2521 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     2581 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     2641 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     2701 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     2761 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     2821 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     2881 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     2941 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
     3001 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     3061 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     3121 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     3181 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     3241 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
     3301 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     3361 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3421 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     3481 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     3541 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     3601 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     3661 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     3721 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     3781 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     3841 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3901 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3961 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4021 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4081 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4141 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4201 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4261 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     4321 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4381 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4441 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4501 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4561 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4621 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4681 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4741 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4801 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4861 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4921 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4981 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5041 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5101 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5161 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5221 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5281 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5341 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5401 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5461 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5521 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5581 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5641 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5701 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5761 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5821 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5881 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5941 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6001 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6061 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6121 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6181 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6241 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     6301 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     6361 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     6421 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     6481 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     6541 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     6601 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     6661 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     6721 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     6781 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6841 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6901 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     6961 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     7021 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     7081 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     7141 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     7201 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     7261 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     7321 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     7381 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     7441 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     7501 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     7561 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     7621 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     7681 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     7741 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     7801 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     7861 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     7921 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     7981 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     8041 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     8101 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     8161 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     8221 aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     8281 ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc atagtcccgc
     8341 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     8401 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     8461 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt tctagaggta
     8521 ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     8581 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     8641 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     8701 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc
     8761 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     8821 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     8881 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     8941 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     9001 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     9061 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     9121 aagcccggtg ccagttaacg aattctagat cttgagacaa atggcagtat tcatccacaa
     9181 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     9241 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     9301 ggtttattac agggacagca gagatccact ttggcgccgg
//
