LOCUS       sequence-313900-        9343 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Allows for constituitive mammalian expression of the IBV 3CL 
            protease via transfection or lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Resnick SJ, Iketani S, Hong SJ, Zask A, Liu H, Kim S, Melore S, Lin 
            FY, Nair MS, Huang Y, Lee S, Tay NES, Rovis T, Yang HW, Xing L, 
            Stockwell BR, Ho DD, Chavez A
  TITLE     Inhibitors of Coronavirus 3CL Proteases Protect Cells from 
            Protease-Mediated Cytotoxicity.
  JOURNAL   J Virol. 2021 Jun 24;95(14):e0237420. doi: 10.1128/JVI.02374-20. 
            Epub 2021 Jun 24.
  PUBMED    33910954
REFERENCE   2  (bases 1 to 9343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2021-06-24"; volume: "95(14):e0237420. doi"; pages: " 
            10.1128/JVI.02374-20. Epub 2021 Jun 24"
COMMENT     Sequence Label: pLEX307-IBV-3CL
FEATURES             Location/Qualifiers
     source          1..9343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2252..2276)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2284..2325
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2376..2964
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2429..2449)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2847..2858)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3036..3269
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3347..3468
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3384..3403)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3438..3457
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3508..3643
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3663..3682)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3664..3682)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3692..3709)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3692..3708)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3701..3723)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3850..4305
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3937..3956)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4147..4168
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4331..4435
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4436..4504,4505..5296)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4654..4673)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5467..6055
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5956..5975
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6209..6226
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6343..6364
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6379..6384,6385..6402,6403..6409)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6417..6433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6422..6444
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6441..6457
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6441..6457
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6476..6496
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6478..6496
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6524..6750
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6751..6931
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6978..7103
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7596..7829
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8014..8058
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8289..8499
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8311..8507
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8358..8493
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8539..9141
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8539..8558)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9035..9055
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9184..9301
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac
     1321 aaaatggctg gttttaagaa actagttgct cctagtagtg ctgttgagaa gtgcattgtt
     1381 agtgtctctt atagaggcag taatcttaat ggattgtggt tgggtgattc catctactgt
     1441 ccacgacatg tattaggtaa gtttagtggt gaccaatgga gtgatgtact tagccttgct
     1501 aataatcatg aatttgaggt tgtaactcat aatggtgtta ctttgaatgt tgtcagcagg
     1561 cgtttaaagg gtgcattact gattttacag actgcagtag ccaatgctga tacaccgaag
     1621 tataaatttt tgaaagcaaa ttgtggtgat agtttcacaa tagcttgctc ttatggtggt
     1681 acagttatag gactctaccc cgttactatg cgttctaatg gaactattag agcatcgttt
     1741 cttgctggag catgtggttc agtaggtttt aatatagaaa agggtgtagt aaatttttac
     1801 tacatgcacc atctagagtt acctaatgca ttacacacag gaactgacct aatgggtgag
     1861 ttttatggtg gttatataga tgaagaggtt gctcagaaag ttcaacccga taaattagtt
     1921 actaataata ttttggcatg gctttatgca gcaattatta gtgttaaaga gagtagtttt
     1981 tcaacaccaa aatggcttga aagtactact atcagtattg atgattataa taagtgggca
     2041 ggtgataatg gttttacatc atttgtaagc tgcactgcta ttactaaatt aagtgctata
     2101 acaggagtag atgtttgtaa actccttcgt actattatgg taaaaagtgc acaatggggt
     2161 agtgacccta ttttgggaca atataatttt gaggatgaaa tgacaccgga atctgttttt
     2221 aatcaggtag gtggtgttag gttacaataa aacccagctt tcttgtacaa agtggttgat
     2281 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     2341 accggttaag tcgacaatca acgcgttaag tcgacaatca acctctggat tacaaaattt
     2401 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     2461 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     2521 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     2581 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     2641 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     2701 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     2761 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     2821 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     2881 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     2941 tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt
     3001 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     3061 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     3121 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     3181 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     3241 cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat
     3301 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     3361 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3421 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     3481 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3541 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     3601 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     3661 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     3721 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     3781 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     3841 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3901 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     3961 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4021 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4081 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4141 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4201 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4261 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     4321 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4381 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4441 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4501 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     4561 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     4621 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     4681 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     4741 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     4801 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     4861 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     4921 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     4981 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5041 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5101 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5161 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5221 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5281 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5341 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5401 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5461 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5521 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5581 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     5641 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5701 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5761 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5821 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     5881 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     5941 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6001 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6061 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6121 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     6181 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     6241 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     6301 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     6361 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     6421 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     6481 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     6541 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     6601 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     6661 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     6721 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     6781 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6841 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6901 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     6961 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     7021 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     7081 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     7141 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     7201 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     7261 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     7321 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     7381 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     7441 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     7501 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     7561 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     7621 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     7681 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     7741 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     7801 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     7861 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     7921 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     7981 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     8041 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     8101 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     8161 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     8221 ccgaggggac ccatgcattg catctcaatt agtcagcaac caggtgtgga aagtccccag
     8281 gctccccagc aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca accatagtcc
     8341 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     8401 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     8461 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctttctagag
     8521 gtaccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     8581 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     8641 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     8701 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg
     8761 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     8821 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     8881 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     8941 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     9001 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     9061 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     9121 cgcaagcccg gtgccagtta acgaattcta gatcttgaga caaatggcag tattcatcca
     9181 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     9241 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     9301 tcgggtttat tacagggaca gcagagatcc actttggcgc cgg
//
