LOCUS       sequence-313906-        9473 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP43
FEATURES             Location/Qualifiers
     source          1..9473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..417
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     347..369
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     385..401
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      430..439
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             439..462
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             475..501
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    517..541
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3587..3611)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3624..4191
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3641..4193
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3785..3802)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4012..4031
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4205..4804
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4205..4224)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4701..4721
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4880..5394
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5556..5572
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5580..5586,5587..5604,5605..5610))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5625..5646
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5763..5780)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5934..6522)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6014..6033)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6693..7484,7485..7553))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7316..7335
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7554..7658)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7726..7744
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7782..7804)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7904..7923
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8117..8139
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8267..8286
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             8486..9002
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    9066..9407
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
ORIGIN
        1 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
       61 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
      121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
      181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
      241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
      301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
      361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccgga
      421 attagatctg ccaccatgga ctacaaggat gacgatgaca agctcgatgg aggataccca
      481 tacgatgttc cagattacgc tgctcgaggt tatcaaacaa gtttgtacaa aaaagttggc
      541 atgtgtttca tgaacgcggt ggtgcagtgt ctcagcaaca ccgacctgct ggccgagttc
      601 ctggcgctgg ggcgctaccg ggcggctccg ggccgcgccg aggtcaccga gcagctggcg
      661 gcgctggtgc gcgcgctctg gactcgcgaa tacacgcccc aactttctgc ggagttcaag
      721 aatgcagttt ccaagtacgg ctctcagttc caaggcaatt cccagcacga cgccctggaa
      781 ttcctgctct ggttgctgga tcgtgtacat gaggacctgg agggttcatc ccgagggccg
      841 gtgtcggaga agcttccgcc tgaagccact aaaacctctg agaactgcct gtcaccatca
      901 gctcagcttc ctctaggtca aagctttgtg caaagccact ttcaagcaca atatagatct
      961 tccttgactt gtccccactg cctgaaacag agcaacacct ttgatccttt cctgtgtgtg
     1021 tccctaccta tccccttgcg ccagacgagg ttcttgagtg tcaccttggt cttcccctct
     1081 aagagccagc ggttcctgcg ggttggcctg gccgtgccga tcctcagcac agtggcagcc
     1141 ctgaggaaga tggttgcaga ggaaggaggc gtccctgcag atgaggtgat cttggttgaa
     1201 ctgtatccca gtggattcca gcggtctttc tttgatgaag aggacctgaa taccatcgca
     1261 gagggagata atgtgtatgc ctttcaagtt cctccctcac ccagccaggg gactctctca
     1321 gctcatccac tgggtctgtc ggcctctcca cgcctggcag cccgtgaggg ccagcgattc
     1381 tccctctctc tccacagtga gagcaaggtg ctaatcctct tctgtaactt ggtggggtca
     1441 gggcagcagg ctagcaggtt tgggccaccc ttcctgataa gggaagacag agctgtttcc
     1501 tgggcccagc tccagcagtc tatcctcagc aaggtccgcc atcttatgaa gagtgaggcc
     1561 cctgtacaga acctggggtc tctgttctcc atccgtgttg tgggactctc tgtggcctgc
     1621 agctatttgt ctccgaagga cagtcggccc ctctgtcact gggcagttga cagggttttg
     1681 catctcagga ggccaggagg ccctccacat gtcaagctgg cggtggagtg ggatagctct
     1741 gtcaaggagc gcctgttcgg gagcctccag gaggagcgag cgcaggatgc cgacagtgtg
     1801 tggcagcagc agcaggcgca tcagcagcac agctgtacct tggatgaatg ttttcagttc
     1861 tacaccaagg aggagcagct ggcccaggat gacgcctgga agtgtcctca ctgccaagtc
     1921 ctgcagcagg ggatggtgaa gctgagtttg tggacgctgc ctgacatcct catcatccac
     1981 ctcaaaaggt tctgccaggt gggcgagaga agaaacaagc tctccacgct ggtgaagttt
     2041 ccgctctctg gactcaacat ggctccccat gtggcccaga gaagcaccag ccctgaggca
     2101 ggactgggcc cctggccttc ctggaagcag ccggactgcc tgcccaccag ttacccgctg
     2161 gacttcctgt acgacctgta tgccgtctgc aaccaccatg gcaacctgca aggtgggcat
     2221 tacacagcct actgccggaa ctctctggat ggccagtggt acagttatga tgacagcacg
     2281 gtggaaccgc ttcgagaaga tgaggtcaac accagagggg cttatatcct gttctatcag
     2341 aagcggaaca gcatccctcc ctggtcagcc agcagctcca tgagaggctc taccagctcc
     2401 tccctgtctg atcactggct cttacggctc gggagccacg ctggcagcac aaggggaagc
     2461 ctgctgtcct ggagctctgc cccctgcccc tccctgcccc aggttcctga ctctcccatc
     2521 ttcaccaaca gcctctgcaa tcaggaaaag ggagggttgg agcccaggcg tttggtacgg
     2581 ggcgtgaaag gcagaagcat tagcatgaag gcacccacca cttcccgagc caagcaggga
     2641 ccattcaaga ccatgcctct gcggtggtcc tttggatcca aggagaaacc accaggtgcc
     2701 tccgtcgagt tggtggagta cttggaatcc agacgaagac ctcggtccac gagccagtcc
     2761 attgtgtcgc tgttgacggg cactgcgggt gaggatgaga agtcagcatc gccgaggtcc
     2821 aacgtcgccc ttcctgctaa cagcgaagat ggtgggcggg ccattgaaag aggtccagcc
     2881 ggggtgccct gtccctcggc tcaacccaac cactgtctgg cccctggaaa ctcagatggt
     2941 ccaaacacag caaggaaact caaggaaaat gcagggcagg acatcaagct tcccagaaag
     3001 tttgacctgc ctctcactgt gatgccttca gtggagcatg agaaaccagc tcgaccggag
     3061 ggccagaagg ccatgaactg gaaggagagc ttccagatgg gaagcaaaag cagcccaccc
     3121 tccccctata tgggattctc tggaaacagc aaagacagtc gccgaggcac ctctgagcta
     3181 gacagacccc tgcaggggac actcaccctt ctgaggtccg tgtttcggaa gaaggagaac
     3241 aggaggaatg agagggcaga ggtctctcca caggtgcccc ccgtctccct ggtgagtggc
     3301 gggctgagcc ctgccatgga cgggcaggct ccaggctcac ctcctgccct caggatccca
     3361 gagggcctgg ccaggggcct gggcagccgg ctcgagaggg atgtctggtc agcccccagc
     3421 tctctccgcc tccctcgtaa agccagcagg gccccgagag gcagtgcact gggcatgtca
     3481 caaaggactg ttccagggga gcaggcttct tatggcacct ttcagagggt caaatatcac
     3541 actctttctt taggtcgaaa gaaaacctta ccggagtcca gcttttgacc aactttcttg
     3601 tacaaagtgg ttcgataacg aattccgccc ccccccccta acgttactgg ccgaagccgc
     3661 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     3721 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     3781 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     3841 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     3901 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     3961 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     4021 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     4081 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     4141 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac
     4201 aaccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     4261 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     4321 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     4381 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     4441 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     4501 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     4561 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     4621 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     4681 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     4741 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     4801 ctgatgcatc gatgtcgacc tgcagccaag cttatcgata aaataaaaga ttttatttag
     4861 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     4921 aacgccattt tgcaaggcat ggaaaataca taactgagaa tagagaagtt cagatcaagg
     4981 ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta agcagttcct
     5041 gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc
     5101 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat
     5161 ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc
     5221 aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc
     5281 gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt
     5341 ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatgggtaa
     5401 cagtttcttg aagttggaga acaacattct gagggtagga gtcgaatatt aagtaatcct
     5461 gactcaatta gccactgttt tgaatccaca tactccaata ctcctgaaat agttcattat
     5521 ggacagcgca gaaagagctg gggagaattg tgaaattgtt atccgctcac aattccacac
     5581 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     5641 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     5701 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     5761 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     5821 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     5881 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5941 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6001 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6061 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6121 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6181 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6241 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6301 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6361 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6421 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6481 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6541 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6601 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6661 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6721 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6781 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6841 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6901 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6961 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7021 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7081 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7141 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7201 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7261 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7321 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7381 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7441 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7501 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7561 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7621 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7681 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     7741 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     7801 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     7861 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     7921 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     7981 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     8041 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     8101 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat
     8161 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     8221 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     8281 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     8341 agaggcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga
     8401 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt
     8461 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     8521 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag
     8581 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
     8641 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag
     8701 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     8761 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     8821 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc
     8881 gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg aatcgtggac
     8941 tcgctgatcc ttgggagggt ctcctcagat tgattgactg cccacctcgg gggtctttca
     9001 tttggaggtt ccaccgagat ttggagaccc ctgcccaggg accaccgacc cccccgccgg
     9061 gaggtaagct ggccagcggt cgtttcgtgt ctgtctctgt ctttgtgcgt gtttgtgccg
     9121 gcatctaatg tttgcgcctg cgtctgtact agttagctaa ctagctctgt atctggcgga
     9181 cccgtggtgg aactgacgag ttctgaacac ccggccgcaa ccctgggaga cgtcccaggg
     9241 actttggggg ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg aatccgaccc
     9301 cgtcaggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
     9361 aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag cgctgcagca
     9421 tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat tag
//
