LOCUS       sequence-313908-        3128 bp    DNA     circular SYN 14-APR-2025
DEFINITION  This plasmid carries Yn situ preamplifier A1-20B1 sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Wu Y, Xu W, Ma L, Yu Z, Wang Y, Yu CR
  TITLE     Robust and sensitive in situ RNA detection using Yn-situ.
  JOURNAL   Cell Rep Methods. 2022 Apr 18;2(4):100201. doi: 
            10.1016/j.crmeth.2022.100201. eCollection 2022 Apr 25.
  PUBMED    35497500
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2022-04-18"; volume: "2(4):100201. doi"; pages: " 
            10.1016/j.crmeth.2022.100201. eCollection 2022 Apr 25"
COMMENT     Sequence Label: A1-20B1 (P001)
FEATURES             Location/Qualifiers
     source          1..3128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1334..1351)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1444..2032)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1524..1543)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2206..3000)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(3001..3105)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatg gagcatgcga cgtcgggccc aattcgcccg cttgtcgttg acgtgacgta
      421 tgattctcta agaggagggc agcaaacggg aagagtcttc ctttacgaag aggagggcag
      481 caaacgggaa gagtcttcct ttacgaagag gagggcagca aacgggaaga gtcttccttt
      541 acgaagagga gggcagcaaa cgggaagagt cttcctttac gaagaggagg gcagcaaacg
      601 ggaagagtct tcctttacga agaggagggc agcaaacggg aagagtcttc ctttacgaag
      661 aggagggcag caaacgggaa gagtcttcct ttacgaagag gagggcagca aacgggaaga
      721 gtcttccttt acgaagagga gggcagcaaa cgggaagagt cttcctttac gaagaggagg
      781 gcagcaaacg ggaagagtct tcctttacga agaggagggc agcaaacggg aagagtcttc
      841 ctttacgaag aggagggcag caaacgggaa gagtcttcct ttacgaagag gagggcagca
      901 aacgggaaga gtcttccttt acgaagagga gggcagcaaa cgggaagagt cttcctttac
      961 gaagaggagg gcagcaaacg ggaagagtct tcctttacga agaggagggc agcaaacggg
     1021 aagagtcttc ctttacgaag aggagggcag caaacgggaa gagtcttcct ttacgaagag
     1081 gagggcagca aacgggaaga gtcttccttt acgaagagga gggcagcaaa cgggaagagt
     1141 cttcctttac gaagaggagg gcagcaaacg ggaagagtct tcctttacgt atagtgagtc
     1201 gtattacaat tcactggccc tgggcctcat gggccttcct ttcactgccc gctttccagt
     1261 cgggaaacct gtcgtgccag ctgcattaac atggtcatag ctgtttcctt gcgtattggg
     1321 cgctctccgc ttcctcgctc actgactcgc tgcgctcggt cgttcgggta aagcctgggg
     1381 tgcctaatga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1441 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1501 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1561 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1621 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1681 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1741 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1801 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1861 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1921 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1981 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2041 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2101 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2161 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttattaga aaaattcatc
     2221 cagcagacga taaaacgcaa tacgctggct atccggtgcc gcaatgccat acagcaccag
     2281 aaaacgatcc gcccattcgc cgcccagttc ttccgcaata tcacgggtgg ccagcgcaat
     2341 atcctgataa cgatccgcca cgcccagacg gccgcaatca ataaagccgc taaaacggcc
     2401 attttccacc ataatgttcg gcaggcacgc atcaccatgg gtcaccacca gatcttcgcc
     2461 atccggcatg ctcgctttca gacgcgcaaa cagctctgcc ggtgccaggc cctgatgttc
     2521 ttcatccaga tcatcctgat ccaccaggcc cgcttccata cgggtacgcg cacgttcaat
     2581 acgatgtttc gcctgatgat caaacggaca ggtcgccggg tccagggtat gcagacgacg
     2641 catggcatcc gccataatgc tcactttttc tgccggcgcc agatggctag acagcagatc
     2701 ctgacccggc acttcgccca gcagcagcca atcacggccc gcttcggtca ccacatccag
     2761 caccgccgca cacggaacac cggtggtggc cagccagctc agacgcgccg cttcatcctg
     2821 cagctcgttc agcgcaccgc tcagatcggt tttcacaaac agcaccggac gaccctgcgc
     2881 gctcagacga aacaccgccg catcagagca gccaatggtc tgctgcgccc aatcatagcc
     2941 aaacagacgt tccacccacg ctgccgggct acccgcatgc aggccatcct gttcaatcat
     3001 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3061 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3121 agtgccac
//
