LOCUS       sequence-313965-        9753 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Prime editing in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Kweon J, Yoon JK, Jang AH, Shin HR, See JE, Jang G, Kim JI, Kim Y
  TITLE     Engineered Prime Editors with PAM flexibility.
  JOURNAL   Mol Ther. 2021 Feb 23. pii: S1525-0016(21)00089-7. doi: 
            10.1016/j.ymthe.2021.02.022.
  PUBMED    33636398
REFERENCE   2  (bases 1 to 9753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2021 Feb 23. pii: S1525-0016(21)00089-7. doi: 
            10.1016/j.ymthe.2021.02.022."
COMMENT     Sequence Label: pCMV-PE2-SpG
FEATURES             Location/Qualifiers
     source          1..9753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             432..452
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             461..478
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(501..518)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    507..731
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(802..818)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(802..818)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(815..837)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    826..842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(850..856,857..874,875..880))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    895..916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1033..1050)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1204..1792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1284..1303)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1963..2754,2755..2823))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2586..2605
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2824..2928)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3007..3026)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3198..3577
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3578..3781
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3732..3752
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3823..3842
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3823..3841
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3891..3911
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3912..8012
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gggctaaagc cctgccagcc gggacatccg ctcagcgggc tgaactgata gcactcaccc
       61 aggccctaaa gatggcagaa ggtaagaagc taaatgttta tactgatagc cgttatgctt
      121 ttgctactgc ccatatccat ggagaaatat acagaaggcg tgggtggctc acatcagaag
      181 gcaaagagat caaaaataaa gacgagatct tggccctact aaaagccctc tttctgccca
      241 aaagacttag cataatccat tgtccaggac atcaaaaggg acacagcgcc gaggctagag
      301 gcaaccggat ggctgaccaa gcggcccgaa aggcagccat cacagagact ccagacacct
      361 ctaccctcct catagaaaat tcatcaccct ctggcggctc aaaaagaacc gccgacggca
      421 gcgaattcga gcccaagaag aagaggaaag tctaaccggt catcatcacc atcaccattg
      481 agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
      541 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
      601 aaatgagaaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
      661 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
      721 gggctctatg gcttctgagg cggaaagaac cagctggggc tcgataccgt cgacctctag
      781 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
      841 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctagggtg cctaatgagt
      901 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
      961 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1021 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1081 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1141 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1201 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1261 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1321 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1381 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1441 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1501 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1561 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1621 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1681 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1741 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1801 tttgatcttt tctacggggt ctgacactca gtggaacgaa aactcacgtt aagggatttt
     1861 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1921 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     1981 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2041 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2101 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2161 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2221 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2281 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2341 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2401 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2461 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2521 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2581 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2641 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2701 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2761 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2821 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     2881 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     2941 aaaagtgcca cctgacgtcg acggatcggg agatcgatct cccgatcccc tagggtctac
     3001 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     3061 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     3121 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     3181 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
     3241 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     3301 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     3361 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     3421 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     3481 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     3541 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     3601 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     3661 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     3721 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     3781 tggtttagtg aaccgtcaga tccgctagag atccgcggcc gctaatacga ctcactatag
     3841 ggagagccgc caccatgaaa cggacagccg acggaagcga gttcgagtca ccaaagaaga
     3901 agcggaaagt cgacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct
     3961 gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca
     4021 ccgaccggca cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa
     4081 cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc
     4141 ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct
     4201 tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca
     4261 tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc
     4321 tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc
     4381 tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca
     4441 acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg
     4501 aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca
     4561 agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt
     4621 tcggaaacct gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc
     4681 tggccgagga tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc
     4741 tgctggccca gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg
     4801 acgccatcct gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga
     4861 gcgcctctat gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc
     4921 tcgtgcggca gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg
     4981 gctacgccgg ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc
     5041 ccatcctgga aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc
     5101 tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag
     5161 agctgcacgc cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg
     5221 aaaagatcga gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg
     5281 gaaacagcag attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact
     5341 tcgaggaagt ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact
     5401 tcgataagaa cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact
     5461 tcaccgtgta taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg
     5521 ccttcctgag cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga
     5581 aagtgaccgt gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg
     5641 tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc
     5701 tgaaaattat caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag
     5761 atatcgtgct gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa
     5821 cctatgccca cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg
     5881 gctggggcag gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga
     5941 caatcctgga tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc
     6001 acgacgacag cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg
     6061 atagcctgca cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc
     6121 tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga
     6181 acatcgtgat cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc
     6241 gcgagagaat gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag
     6301 aacaccccgt ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga
     6361 atgggcggga tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg
     6421 tggacgctat cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga
     6481 ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga
     6541 agatgaagaa ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg
     6601 acaatctgac caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca
     6661 agagacagct ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc
     6721 ggatgaacac taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc
     6781 tgaagtccaa gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga
     6841 tcaacaacta ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga
     6901 tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg
     6961 tgcggaagat gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct
     7021 tctacagcaa catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc
     7081 ggaagcggcc tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc
     7141 gggattttgc caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga
     7201 ccgaggtgca gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata
     7261 agctgatcgc cagaaagaag gactgggacc ctaagaagta cggcggcttc ctgtggccca
     7321 ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga
     7381 agagtgtgaa agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc
     7441 ccatcgactt tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc
     7501 tgcctaagta ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgcca
     7561 agcagctgca gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc
     7621 tggccagcca ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt
     7681 ttgtggaaca gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca
     7741 agagagtgat cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc
     7801 gggataagcc catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc
     7861 tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag cagtacagaa
     7921 gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga
     7981 cacggatcga cctgtctcag ctgggaggtg actctggagg atctagcgga ggatcctctg
     8041 gcagcgagac accaggaaca agcgagtcag caacaccaga gagcagtggc ggcagcagcg
     8101 gcggcagcag caccctaaat atagaagatg agtatcggct acatgagacc tcaaaagagc
     8161 cagatgtttc tctagggtcc acatggctgt ctgattttcc tcaggcctgg gcggaaaccg
     8221 ggggcatggg actggcagtt cgccaagctc ctctgatcat acctctgaaa gcaacctcta
     8281 cccccgtgtc cataaaacaa taccccatgt cacaagaagc cagactgggg atcaagcccc
     8341 acatacagag actgttggac cagggaatac tggtaccctg ccagtccccc tggaacacgc
     8401 ccctgctacc cgttaagaaa ccagggacta atgattatag gcctgtccag gatctgagag
     8461 aagtcaacaa gcgggtggaa gacatccacc ccaccgtgcc caacccttac aacctcttga
     8521 gcgggctccc accgtcccac cagtggtaca ctgtgcttga tttaaaggat gcctttttct
     8581 gcctgagact ccaccccacc agtcagcctc tcttcgcctt tgagtggaga gatccagaga
     8641 tgggaatctc aggacaattg acctggacca gactcccaca gggtttcaaa aacagtccca
     8701 ccctgtttaa tgaggcactg cacagagacc tagcagactt ccggatccag cacccagact
     8761 tgatcctgct acagtacgtg gatgacttac tgctggccgc cacttctgag ctagactgcc
     8821 aacaaggtac tcgggccctg ttacaaaccc tagggaacct cgggtatcgg gcctcggcca
     8881 agaaagccca aatttgccag aaacaggtca agtatctggg gtatcttcta aaagagggtc
     8941 agagatggct gactgaggcc agaaaagaga ctgtgatggg gcagcctact ccgaagaccc
     9001 ctcgacaact aagggagttc ctagggaagg caggcttctg tcgcctcttc atccctgggt
     9061 ttgcagaaat ggcagccccc ctgtaccctc tcaccaaacc ggggactctg tttaattggg
     9121 gcccagacca acaaaaggcc tatcaagaaa tcaagcaagc tcttctaact gccccagccc
     9181 tggggttgcc agatttgact aagccctttg aactctttgt cgacgagaag cagggctacg
     9241 ccaaaggtgt cctaacgcaa aaactgggac cttggcgtcg gccggtggcc tacctgtcca
     9301 aaaagctaga cccagtagca gctgggtggc ccccttgcct acggatggta gcagccattg
     9361 ccgtactgac aaaggatgca ggcaagctaa ccatgggaca gccactagtc attctggccc
     9421 cccatgcagt agaggcacta gtcaaacaac cccccgaccg ctggctttcc aacgcccgga
     9481 tgactcacta tcaggccttg cttttggaca cggaccgggt ccagttcgga ccggtggtag
     9541 ccctgaaccc ggctacgctg ctcccactgc ctgaggaagg gctgcaacac aactgccttg
     9601 atatcctggc cgaagcccac ggaacccgac ccgacctaac ggaccagccg ctcccagacg
     9661 ccgaccacac ctggtacacg gatggaagca gtctcttaca agagggacag cgtaaggcgg
     9721 gagctgcggt gaccaccgag accgaggtaa tct
//
