LOCUS       sequence-313977-        2178 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0391
FEATURES             Location/Qualifiers
     source          1..2178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     39..56
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(80..137)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      291..334
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(338..356)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             518..1177
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(584..603)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1198..1292
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1475..2063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1964..1983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
       61 cctgcataac gcgaagtaat cttttcggtt ttaaagaaaa agggcagggt ggtgacacct
      121 tgcccttttt tgccggactg cagggtctcg agtacattat atacaaatca gtcactactg
      181 ctttcaatga atagcaaaac cttatatagc gcaataatgg ttcgcaataa tggttcgcaa
      241 taatggttcc tcactaaaac gacacgcctc cctcctgaga ccgaattcca gaaatcatcc
      301 ttagcgaaag ctaaggattt tttttatctg aaattctgcc tcgtgatacg cctattttta
      361 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcactcg agttgatcgg
      421 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
      481 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
      541 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
      601 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
      661 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
      721 tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
      781 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
      841 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
      901 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
      961 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     1021 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     1081 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     1141 acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac
     1201 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg
     1261 gttgccgccg ggcgtttttt attggtgaga atccaagcct cgagctgtca gaccaagttt
     1321 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1381 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1441 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1501 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1561 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1621 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1681 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1741 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1801 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1861 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1921 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1981 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2041 caggggggcg gagcctgtgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2101 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2161 gtattaccgc ctttgagt
//
