LOCUS       sequence-313978-        2356 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0211
FEATURES             Location/Qualifiers
     source          1..2356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(59..153)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(174..833)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     748..767
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     995..1013
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1017..1060)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1392..1449
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1473..1490)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1644..2232)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1724..1743)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat
       61 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
      121 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
      181 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
      241 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
      301 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
      361 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
      421 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
      481 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
      541 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
      601 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
      661 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
      721 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
      781 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
      841 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
      901 ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta
      961 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt
     1021 cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgg tctcaggagg
     1081 gaggcgtgtc gttttagtga ggtatcgtcc tttgaagata agataataat gttgaagata
     1141 agagtgggag ccaccactaa aacattgctt tgtcaaaagc taaaaaagat gatgcccgac
     1201 agccagacga aggaaatgac gtaagccatg acgtctaatc ccacaagaaa gcccaggtcg
     1261 tgttgattcg gattttgtaa aatgggttct ggcgtcttta tggaggttct ccatctgttt
     1321 tactatataa ggttttgcta ttcattgaaa gcagtagtga ctgatttgta tatatactcg
     1381 agaccctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
     1441 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
     1501 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1561 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1621 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
     1681 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1741 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1801 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1861 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1921 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1981 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2041 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2101 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2161 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2221 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2281 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2341 ttaaattaaa aatgaa
//
