LOCUS       sequence-31400-e         912 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SGF29 (3MEU)
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(577..588)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             663..680
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(733..751)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      747..794
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attcctctga atattttgtt tactttaaga aggagatata ccatgcatca tcatcatcat
       61 cacagcagcg gcagagaaaa cttgtatttc cagggccgca gaggggtgct gatgaccctg
      121 ctgcagcagt cggccatgac cctgcccctg tggatcggga agcctggtga caagccccca
      181 cccctctgtg gggccatccc tgcctcagga gactacgtgg ccagacctgg agacaaggtg
      241 gctgcccggg tgaaggccgt ggatggggac gagcagtgga tcctggccga ggtggtcagt
      301 tacagccatg ccaccaacaa gtatgaggta gatgacatcg atgaagaagg caaagagaga
      361 cacaccctga gccggcgccg tgtcatcccg ctgccccagt ggaaggccaa cccggagacg
      421 gaccctgagg ccttgttcca gaaggagcag ctcgtgctgg ccctgtatcc ccagactacc
      481 tgcttctacc gcgccctgat ccatgcgccc ccacagcggc cccaggatga ctactcggtc
      541 ctgtttgaag acacctccta tgcagatggc tattcccctc ccctcaatgt ggctcagaga
      601 tacgtggtgg cttgtaagga acccaagaaa aagtgatgac gaagcttgcg gccgcactcg
      661 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
      721 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
      781 tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg
      841 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc acgtgacgga ccgctaactt
      901 gccagcgccc ta
//
