LOCUS       sequence-314041-        8204 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   Facchini LM, Chen S, Marhin WW, Lear JN, Penn LZ
  TITLE     The Myc negative autoregulation mechanism requires Myc-Max 
            association and involves the c-myc P2 minimal promoter.
  JOURNAL   Mol Cell Biol. 1997 Jan;17(1):100-14.
  PUBMED    8972190
REFERENCE   2  (bases 1 to 8204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "1997-01"; volume: "17(1)"; pages: "100-14"
COMMENT     Sequence Label: HBM-Luc
FEATURES             Location/Qualifiers
     source          1..8204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2561..2577)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2561..2577)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             2658..4310
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2724..2742)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          4551..4616
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4746..4766
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5191..5325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5467..5484)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5638..6226)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5718..5737)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6397..7188,7189..7257))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7020..7039
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7258..7362)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7389..7844
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7476..7495)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7686..7707
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    8034..8155
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     join(8204,1..16)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cgaggtcgac ggtatcgata agcttgtttg gccgttttag ggtttgttgg aatttttttt
       61 tcgtctatgt acttgtgaat tatttcacgt ttgccattac cggttctcca tagggtgatg
      121 ttcattagca gtggtgatag gttaattttc accatctctt atgcggttga atagtcacct
      181 ctgaaccact ttttcctcca gtaactcctc tttcttcgga ccttctgcag ccaacctgaa
      241 agaataacaa ggaggtggct ggaaacttgt tttaaggaac cgcctgtcct tcccccgctg
      301 gaaaccttgc acctcggacg ctcctgctcc tgcccccacc tgacccccgc cctcgttgac
      361 atccaggcgc gatgatctct gctgccagta gagggcacac ttactttact ttcgcaaacc
      421 tgaacgcggg tgctgcccag agagggggcg gagggaaaga cgctttgcag caaaatccag
      481 catagcgatt ggttgctccc cgcgtttgcg gcaaaggcct ggaggcagga gtaatttgca
      541 atccttaaag ctgaattgtg cagtgcatcg gatttggaag ctactatatt cacttaacac
      601 ttgaacgctg agctgcaaac tcaacgggta ataacccatc ttgaacagcg tacatgctat
      661 acacgcaccc ctttcccccg aattgttttc tcttttggag gtggtggagg gagagaaaag
      721 tttacttaaa atgcctttgg gtgagggacc aaggatgaga agaatgtttt ttgtttttca
      781 tgccgtggaa taacacaaaa taaaaaatcc cgagggaata tacattatat attaaatata
      841 gatcatttca gggagcaaac aaatcatgtg tggggctggg caactagcta agtcgaagcg
      901 taaataaaat gtgaatacac gtttgcgggt tacatacagt gcactttcac tagtattcag
      961 aaaaaattgt gagtcagtga actaggaaat taatgcctgg aaggcagcca aattttaatt
     1021 agctcaagac tccccccccc cccaaaaaaa ggcacggaag taatactcct ctcctcttct
     1081 ttgatcagaa tcgatgcatt ttttgtgcat gaccgcattt ccaataataa aaggggaaag
     1141 aggacctgga aaggaattaa acgtccggtt tgtccgggga ggaaagagtt aacggttttt
     1201 ttcacaaggg tctctgctga ctcccccggc tcggtccaca agctctccac ttgccccttt
     1261 taggaagtcc ggtcccgcgg ttcgggtacc ccctgcccct cccatattct cccgtctagc
     1321 acctttgatt tctcccaaac ccggcagccc gagactgttg caaaccggcg ccacagggcg
     1381 caaaggggat ttgtctcttc tgaaacctgg ctgagaaatt gggaactccg tgtgggaggc
     1441 gtgggggtgg gacggtgggg tacagactgg cagagagcag gcaacctccc tctcgcccta
     1501 gcccagctct ggaacaggca gacacatctc agggctaaac agacgcctcc cgcacggggc
     1561 cccacggaag cctgagcagg cggggcagga ggggcggtat ctgctgcttt ggcagcaaat
     1621 tgggggactc agtctgggtg gaaggtatcc aatccagata gctgtgcata cataatgcat
     1681 aatacatgac tccccccaac aaatgcaatg ggagtttatt cataacgcgc tctccaagta
     1741 tacgtggcaa tgcgttgctg ggttatttta atcattctag gcatcgtttt cctccttatg
     1801 cctctatcat tcctccctat ctacactaac atcccacgct ctgaacgcgc gcccattaat
     1861 acccttcttt cctccactct ccctgggact cttgatcaaa gcgcggccct ttccccagcc
     1921 ttagcgaggc gccctgcagc ctggtacgcg cgtggcgtgg cggtgggcgc gcagtgcgtt
     1981 ctcggtgtgg agggcagctg ttccgcctgc gatgatttat actcacagga caaggatgcg
     2041 gtttgtcaaa cagtactgct acggaggagc agcagagaaa gggagagggt ttgagaggga
     2101 gcaaaagaaa atggtaggcg cgcgtagtta attcatgcgg ctctcttact ctgtttacat
     2161 cctagagcta gagtgctcgg ctgcccggct gagtctcctc cccaccttcc ccaccctccc
     2221 caccctcccc ataagcgccc ctcccgggtt cccaaagcag agggcgtggg ggaaaagaaa
     2281 aaagatcctc tctcgctaat ctccgcccac cggcccttta taatgcgagg gtctggacgg
     2341 ctgaggaccc ccgagctgtg ctgctcgcgg ccgccaccgc cgggccccgg ccgtccctgg
     2401 ctcccctcct gcctcgagaa gggcagggct tctcagaggc ttggcgggaa aaagaacgga
     2461 gggagggatc gcgctgagta taaaagccgg ttttcggggc tttatctaac tcgctgtagt
     2521 aattccagcg agaggcagag ggagcgagcg ggcggccccg gatccactag ttctagagcg
     2581 gccgccaccg cggtggagct cttacgcgtg ctagctcgag atctaagtaa gcttggcatt
     2641 ccggtactgt tggtaaaatg gaagacgcca aaaacataaa gaaaggcccg gcgccattct
     2701 atcctctaga ggatggaacc gctggagagc aactgcataa ggctatgaag agatacgccc
     2761 tggttcctgg aacaattgct tttacagatg cacatatcga ggtgaacatc acgtacgcgg
     2821 aatacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg ctgaatacaa
     2881 atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg
     2941 cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc
     3001 tcaacagtat gaacatttcg cagcctaccg tagtgtttgt ttccaaaaag gggttgcaaa
     3061 aaattttgaa cgtgcaaaaa aaattaccaa taatccagaa aattattatc atggattcta
     3121 aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat ctacctcccg
     3181 gttttaatga atacgatttt gtaccagagt cctttgatcg tgacaaaaca attgcactga
     3241 taatgaattc ctctggatct actgggttac ctaagggtgt ggcccttccg catagaactg
     3301 cctgcgtcag attctcgcat gccagagatc ctatttttgg caatcaaatc attccggata
     3361 ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact acactcggat
     3421 atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag ctgtttttac
     3481 gatcccttca ggattacaaa attcaaagtg cgttgctagt accaacccta ttttcattct
     3541 tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa attgcttctg
     3601 ggggcgcacc tctttcgaaa gaagtcgggg aagcggttgc aaaacgcttc catcttccag
     3661 ggatacgaca aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg
     3721 gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg
     3781 atctggatac cgggaaaacg ctgggcgtta atcagagagg cgaattatgt gtcagaggac
     3841 ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg
     3901 atggatggct acattctgga gacatagctt actgggacga agacgaacac ttcttcatag
     3961 ttgaccgctt gaagtcttta attaaataca aaggatatca ggtggccccc gctgaattgg
     4021 aatcgatatt gttacaacac cccaacatct tcgacgcggg cgtggcaggt cttcccgacg
     4081 atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg
     4141 aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag
     4201 gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa
     4261 tcagagagat cctcataaag gccaagaagg gcggaaagtc caaattgtaa aatgtaactg
     4321 tattcagcga tgacgaaatt cttagctatt gtaatactgc gatgagtggc agggcggggc
     4381 gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggttgctacg cctgaataag
     4441 tgataataag cggatgaatg gcagaaattc gccggatctt tgtgaaggaa ccttacttct
     4501 gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag gtaaatataa
     4561 aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc
     4621 caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt
     4681 tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta
     4741 ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa
     4801 gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca
     4861 caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta
     4921 taagtaggca taacagttat aatcataaca tactgttttt tcttactcca cacaggcata
     4981 gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta
     5041 aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat
     5101 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     5161 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     5221 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5281 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgtcg accgatgccc
     5341 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     5401 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc
     5461 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5521 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5581 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5641 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5701 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5761 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5821 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5881 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5941 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6001 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6061 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6121 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6181 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6241 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6301 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6361 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     6421 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6481 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6541 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6601 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6661 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6721 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6781 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6841 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6901 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6961 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7021 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7081 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7141 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7201 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7261 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     7321 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     7381 gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     7441 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     7501 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     7561 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     7621 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     7681 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     7741 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     7801 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttgccatt cgccattcag
     7861 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagcccaa
     7921 gctaccatga taagtaagta atattaaggt acgtggaggt tttacttgct ttaaaaaacc
     7981 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     8041 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8101 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatggta
     8161 ctgtaactga gctaacataa cccgggaggt accgggcccc ccct
//
