LOCUS       sequence-31423-e         882 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ECT2
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             398..415
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(468..486)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      482..529
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(653..672)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aatattttgt ttactttaga aggagatata ccatgcatca tcatcatcat cacagcagcg
       61 gcagagaaaa cttgtatttc catggcgttc ctccatttca agattgtatt ttaagtttcc
      121 tgggattttc agatgaagag aaaaccaata tggaagaaat gactgaaatg caaggaggta
      181 aatatttacc gcttggagat gaaagatgca ctcaccttgt agttgaagag aatatagtaa
      241 aagatcttcc ctttgaacct tcaaagaaac tttatgttgt caagcaagag tggttctggg
      301 gaagcattca aatggatgcc cgagctggag aaactatgta tttatatgaa aaggcaaata
      361 ctcctgagtg atgacgaagc ttgcggccgc actcgagcac caccaccacc accactgaga
      421 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      481 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      541 aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
      601 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      661 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
      721 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
      781 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
      841 ttggagtcca cgttctttaa tagtggactc ttgttcccaa ct
//
