LOCUS       sequence-314767-        1696 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin 
            dependent fluorescent protein fused to the C-terminus of Aga2p 
            protein. This plasmid contains a long 2G linker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Zahradnik J, Dey D, Marciano S, Schreiber G
  TITLE     An enhanced yeast display platform demonstrates the binding 
            plasticity under various selection pressures
  JOURNAL   bioRxiv Preprint 2020
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            Preprint 2020"
COMMENT     Sequence Label: pJYDNgp
FEATURES             Location/Qualifiers
     source          1..1696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        181..622
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    181..298
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     593..616
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             1101..1112
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1113..1139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1206..1235
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cgccagcaac gcggcacttt gtaactgagc tgtcattttg ctggcccagc tggtcgaccg
       61 cggctaagca gtactggtac cgttgtggaa atgtaaagag ccccattatc ttagcctaaa
      121 aaaaccttct ctttggaact ttcagtaata cgcttaactg ctcattgcta tattgaagta
      181 cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc tccgtgcgtc
      241 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa
      301 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc
      361 tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat
      421 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt
      481 aacagatata taaatgcaaa aactgcataa ccactttaac taatactttc aacattttcg
      541 gtttgtatta cttcttattc aaatgtaata aaagtatcaa caaaaaattg ttaatatacc
      601 tctatacttt aacgtcaagg agaaaaaacc ccggatcgaa ttccctactt catacatttt
      661 caattaagat gaggttccca tctattttca ccgctgttgt ttttgctgct tcttctgctt
      721 tggctgctcc agctaatggt catatggaag catgcttaat taaggcgcct tatctcgagg
      781 ggggcggatc cttctcccaa aagttggaca tcaacttgtt ggataacgtt gtcaactctt
      841 catatcatgg tgaaggtgtt tctggaggtt ctgcacagga actgacaact atatgcgagc
      901 aaatcccctc accaacttta gaatcgacgc cgtactcttt gtcaacgact actattttgg
      961 ccaacgggaa ggcaatgcaa ggagtttttg aatattacaa atcagtaacg tttgtcagta
     1021 attgcggttc tcacccctca acaactagca aaggcagccc cataaacaca cagtatgttt
     1081 ttaaggacaa tagctcgacg attgaaggta gatacccata cgacgttcca gactacgctc
     1141 tgcaggctag tggtggagga ggctctggtg gaggtggtag cggaggcgga gggtcggcta
     1201 gccatgaaca gaagttgatc tctgaagaag atttgatgtt agaaaaattt gttggcacct
     1261 ggaagatcga atcctctgaa aattttggtg aatacttgaa ggctatcggt gccccaaaag
     1321 aattggctga tgctggtgat gctactactc cagtcttgta catttctcaa aaggatggtg
     1381 ataagatgac cgtcaagatt gaaaacggtc caccaacttt tttggatacc caagtttctt
     1441 tcaagttggg tgaagaattc gacgaatttc catccgatag aagaaagggt gttaagtccg
     1501 ttgttaactt gtctggtgag aagttggttt acgttcaaaa gtgggatggt aaagaaacca
     1561 cttacgtcag agaaatcaag gacggtaaat tggttgttac tttgaccatg ggtgatgttg
     1621 ttgctgttag atcttataga agggcctctg aataatagag atctgataac aacagtgtag
     1681 atgtaacaaa atcgac
//
