LOCUS       sequence-31479-e         861 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FTSJ2
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             705..722
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(775..793)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      789..836
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggttccgcg tggtagtagt taccggagcc gaagcgcctt caagctcctg
      121 gaggtgaacg agaggcacca gattctgcgg cccggccttc gggtgttaga ctgtggggca
      181 gctcctgggg cctggagtca ggtggcggtg cagaaggtca acgccgcagg cacagatccc
      241 agctctcctg ttggcttcgt gcttggggta gatcttcttc acatattccc cctggaagga
      301 gcaacttttc tgtgccctgc tgacgtgact gacccgagaa cctcacagag aatcctcgag
      361 gtgcttcctg gcaggagagc agatgtgatt ctgagcgaca tggcgcccaa tgccacaggg
      421 ttccgggacc tcgatcatga caggctcatc agcctgtgcc tgacccttct cagcgtgacc
      481 ccagacatcc tgcaacctgg ggggacattc ctttgtaaaa cctgggctgg aagtcaaagc
      541 cgtcggttac agaggagact gacagaggaa ttccagaatg taaggatcat caaacctgaa
      601 gccagcagga aagagtcatc agaagtgtac ttcttggcca cacagtacca cggaaggaag
      661 ggcactgtga agcagtgatg acgaagcttg cggccgcact cgagcaccac caccaccacc
      721 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      781 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      841 aaggaggaac tatatccgga t
//
