LOCUS       sequence-314835-        5141 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of c-luciferase from sp. Maristella sp. SVU (Belize).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Hensley NM, Ellis EA, Leung NY, Coupart J, Mikhailovsky A, Taketa 
            DA, Tessler M, Gruber DF, De Tomaso AW, Mitani Y, Rivers TJ, Gerrish
            GA, Torres E, Oakley TH
  TITLE     Selection, drift, and constraint in cypridinid luciferases and the 
            diversification of bioluminescent signals in sea fireflies.
  JOURNAL   Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.15673.
  PUBMED    33031624
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ecol. 
            2020 Oct 8. doi: 10.1111/mec.15673."
COMMENT     Sequence Label: pPICZ-aC-MSVU-BZ
FEATURES             Location/Qualifiers
     source          1..5141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             join(941..997,998..1195)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKR"
     CDS             2822..2851
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2867..2884
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2964..3210
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        3225..3638
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        3645..3692
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3711..4085
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      4151..4398
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4167..4185)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(4473..5061)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4553..4572)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg atgagatttc cttcaatttt
      961 tactgctgtt ttattcgcag catcctccgc attagctgct ccagtcaaca ctacaacaga
     1021 agatgaaacg gcacaaattc cggctgaagc tgtcatcggt tactcagatt tagaagggga
     1081 tttcgatgtt gctgttttgc cattttccaa cagcacaaat aacgggttat tgtttataaa
     1141 tactactatt gccagcattg ctgctaaaga agaaggggta tctctcgaga agagagaggc
     1201 tcaagattgt tatgaattca catgggcttc gaatgatttt ccatcttcat gtgaggctat
     1261 aaacggaaga tgtgtagact ctgcgtgcgg tagctgtgac gaagttctgt ttggcgatgg
     1321 attgtgtgaa aatgccggag gagcatctcc caagtgctgt agagattgcc cagaaatcgt
     1381 caggtgcaga gcttcagcag ctggattttt tcacacgttc tatgggcaga gattcaatct
     1441 ccaggtacca gggacctatc tgctgagtga agactgcgtg ggaggactat ggtctctata
     1501 tgttaacctg gcgaatatag aaggagagaa gggagccgtt ttagattccg ttaagatggt
     1561 tgttggagac gtaactctag atattaagca gaaagttgga tctattacgg taaatggagg
     1621 ttctgttgaa atcgacagca atccattctc tatcggtgac gtcaccatcg ccgtcgtata
     1681 cactccttac tttagcgtct ccgtcatcga gtttcttaag cttgttacat ttgatatctt
     1741 acaaggcaga gctttccgac ttgctccaga cttcttatac gctgatagga cctgtggctt
     1801 atgtggattg atgtccgatg aaccttctga ttttattgac gatccagatc aacttgcaat
     1861 ccaagacata atgaatcaag atgtagaagg ctgcccacta tctggaaatc cttctgacgc
     1921 cgagtattgc ttgaataaga tgcagccata taaggacggc tgtattaaca agaacgatgt
     1981 tcacttcggt acctacctgt acgcatgcgc actggcctac tgcatgggcg gcgacgatag
     2041 agtagaggat gttatttttg aatacgccga agcctgcgtt gaaccaatcg gtagagcaac
     2101 ctgcgttatg aatggacaca catactatga cacattcgac aaatcaagtt atcagtttca
     2161 ggcaccttgt aaggttctat tcgcgaaaga ctgtacagat gatgaatggg aagtaactat
     2221 tactcataaa gaagctggag aatatactga agtagaaaag gtcaccgtcc ggtatttcca
     2281 aaccttgatt gatctggtag cagagaataa gaaagttttt gttaatggaa ctgaagttag
     2341 cgtcccttac aactatggag atacatctat atacatgtat gataacttag taactacagc
     2401 agttctacca ggagcagtgg ttgtcaaata caacttcgag cagatgttgg ctctacacat
     2461 tagagatcca tatgaagcag acagctgtgg tttatgtggt atttgggatt tggataaatc
     2521 taatgatggt cctgatgctc agcatgcaga ttgtgaacca actcccaacc ctcctacatg
     2581 cactgctgac aaagaggctg aagctagaga actctgccag aatatgtttc ctgcttctat
     2641 cgacgacaaa tgtaacatct gctacaaggc tgaccgtgtt gagcgctgta tgtatgagta
     2701 ctgtcttggt ggtctagaag cattctgtca acatgctggt accgtcatcg atgaatgctt
     2761 cgtgaggcac ggagacgacc tacaatatcc ccctcagtgt aaagcggccg ccagctttct
     2821 agaacaaaaa ctcatctcag aagaggatct gaatagcgcc gtcgaccatc atcatcatca
     2881 tcattgagtt tgtagcctta gacatgactg ttcctcagtt caagttgggc acttacgaga
     2941 agaccggtct tgctagattc taatcaagag gatgtcagaa tgccatttgc ctgagagatg
     3001 caggcttcat ttttgatact tttttatttg taacctatat agtataggat tttttttgtc
     3061 attttgtttc ttctcgtacg agcttgctcc tgatcagcct atctcgcagc tgatgaatat
     3121 cttgtggtag gggtttggga aaatcattcg agtttgatgt ttttcttggt atttcccact
     3181 cctcttcaga gtacagaaga ttaagtgaga ccttcgtttg tgcggatccc ccacacacca
     3241 tagcttcaaa atgtttctac tcctttttta ctcttccaga ttttctcgga ctccgcgcat
     3301 cgccgtacca cttcaaaaca cccaagcaca gcatactaaa tttcccctct ttcttcctct
     3361 agggtgtcgt taattacccg tactaaaggt ttggaaaaga aaaaagagac cgcctcgttt
     3421 ctttttcttc gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt cttgaaaatt
     3481 tttttttttg atttttttct ctttcgatga cctcccattg atatttaagt taataaacgg
     3541 tcttcaattt ctcaagtttc agtttcattt ttcttgttct attacaactt tttttacttc
     3601 ttgctcatta gaaagaaagc atagcaatct aatctaaggg cggtgttgac aattaatcat
     3661 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     3721 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     3781 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     3841 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     3901 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     3961 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     4021 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     4081 actgacacgt ccgacgcggc ccgacgggtc cgaggcctcg gagatccgtc ccccttttcc
     4141 tttgtcgata tcatgtaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc
     4201 gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt
     4261 atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac
     4321 agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc
     4381 tcgaaggctt taatttgcaa gctggagacc aacatgtgag caaaaggcca gcaaaaggcc
     4441 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4501 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4561 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4621 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4681 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4741 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4801 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4861 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     4921 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4981 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5041 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5101 tggaacgaaa actcacgtta agggattttg gtcatgagat c
//
