LOCUS       sequence-314846-        1721 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Positive control plasmid for pJYDN3n plasmid expression attempts. It
            contains both eUnaG2 bilirubin dependent fluorescent protein and 
            ALFA-tag for DnbALFA based detection on the cell surface..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Zahradnik J, Dey D, Marciano S, Schreiber G
  TITLE     An enhanced yeast display platform demonstrates the binding 
            plasticity under various selection pressures
  JOURNAL   bioRxiv Preprint 2020
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            Preprint 2020"
COMMENT     Sequence Label: pJYDN3p
FEATURES             Location/Qualifiers
     source          1..1721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        182..623
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    182..299
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     594..617
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             745..783
                     /codon_start=1
                     /product="rationally designed epitope tag that forms a 
                     stable alpha-helix (Gotzke et al., 2019)"
                     /label=ALFA-tag
                     /note="It may be beneficial to flank the ALFA-tag sequence 
                     with proline residues to preserve the helical structure."
                     /translation="SRLEEELRRRLTE"
     CDS             1126..1137
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1138..1164
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1231..1260
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 acgccagcaa cgcggcactt tgtaactgag ctgtcatttt gctggcccag ctggtcgacc
       61 gcggctaagc agtactggta ccgttgtgga aatgtaaaga gccccattat cttagcctaa
      121 aaaaaccttc tctttggaac tttcagtaat acgcttaact gctcattgct atattgaagt
      181 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
      241 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      301 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      361 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      421 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      481 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      541 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      601 ctctatactt taacgtcaag gagaaaaaac cccggatcga attccctact tcatacattt
      661 tcaattaaga tgaggttccc atctattttc accgctgttg tttttgctgc ttcttctgct
      721 ttggctgctc cagctaatgg tccatccaga ttggaagaag aattgagaag aagattaacc
      781 gaaccacata tggaagcatg cttaattaag gcgccttatc tcgagggggg cggatcctct
      841 ggtggaggtg gttcgggcgg aggtggtaac ggatctaatg gttctggcgg tagctctgca
      901 caggaactga caactatatg cgagcaaatc ccctcaccaa ctttagaatc gacgccgtac
      961 tctttgtcaa cgactactat tttggccaac gggaaggcaa tgcaaggagt ttttgaatat
     1021 tacaaatcag taacgtttgt cagtaattgc ggttctcacc cctcaacaac tagcaaaggc
     1081 agccccataa acacacagta tgtttttaag gacaatagct cgacgattga aggtagatac
     1141 ccatacgacg ttccagacta cgctctgcag gctagtggtg gaggaggctc tggtggaggt
     1201 ggtagcggag gcggagggtc ggctagccat gaacagaagt tgatctctga agaagatttg
     1261 atgttagaaa aatttgttgg cacctggaag atcgaatcct ctgaaaattt tggtgaatac
     1321 ttgaaggcta tcggtgcccc aaaagaattg gctgatgctg gtgatgctac tactccagtc
     1381 ttgtacattt ctcaaaagga tggtgataag atgaccgtca agattgaaaa cggtccacca
     1441 acttttttgg atacccaagt ttctttcaag ttgggtgaag aattcgacga atttccatcc
     1501 gatagaagaa agggtgttaa gtccgttgtt aacttgtctg gtgagaagtt ggtttacgtt
     1561 caaaagtggg atggtaaaga aaccacttac gtcagagaaa tcaaggacgg taaattggtt
     1621 gttactttga ccatgggtga tgttgttgct gttagatctt atagaagggc ctctgaataa
     1681 tagagatctg ataacaacag tgtagatgta acaaaatcga c
//
