LOCUS       sequence-31491-e         889 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CDYL
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     269..288
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     372..391
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        372..390
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    391..415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             430..452
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             471..488
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             498..515
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
       61 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
      121 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
      181 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
      241 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
      301 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga
      361 tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
      421 agaaataatt ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc
      481 atcatcacag cagcggcctg gttccgcgtg gtagtgccta cagatacaga gatattgtgg
      541 tcaggaagca ggatggcttc acccacatct tgttatccac aaagtcctca gagaataact
      601 cactaaatcc agaggtaatg agagaagtcc agagtgctct gagcacggcc gctgccgatg
      661 acagcaagct ggtactgctc agcgccgttg gcagcgtctt ctgttgtgga cttgacttta
      721 tttattttat acgacgtctg acagatgaca ggaaaagaga aagcactaaa atggcagaag
      781 ctatcagaaa cttcgtgaat actttcattc aatttaagaa gcccattatt gtagcagtca
      841 atggcccagc cattggtcta ggagcatcta tattgcctct ttgcgatgt
//
