LOCUS       sequence-314933-        3510 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Estradiol inducible system trans activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: lvl0 sXVE
FEATURES             Location/Qualifiers
     source          1..3510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      4..61
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(85..102)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(256..844)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(336..355)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1027..1121)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1142..1801)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1716..1735
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1963..1981
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1985..2028)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
       61 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      121 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      181 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      241 cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg
      301 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      361 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      421 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      481 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      541 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      601 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      661 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      721 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      781 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      841 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      901 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      961 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag
     1021 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     1081 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     1141 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     1201 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     1261 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     1321 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     1381 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     1441 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     1501 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     1561 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     1621 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     1681 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     1741 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     1801 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     1861 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct
     1921 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     1981 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcggtc
     2041 tctaatgaag gctcttactg ctagacaaca agaagttttt gatcttatta gagatcatat
     2101 ttctcaaact ggaatgcctc ctactagagc tgaaattgct caaagacttg gatttagatc
     2161 tcctaatgct gctgaagaac atcttaaggc tcttgctaga aagggagtta ttgaaattgt
     2221 ttctggagct tcaagaggaa ttagacttct tcaagaagaa gaagaaggac ttcctcttgt
     2281 tggaagagtt gctgctggag aaccttcttc tgctcctcct actgatgttt ctcttggaga
     2341 tgaacttcat cttgatggag aagatgttgc tatggctcat gctgatgctc ttgatgattt
     2401 tgatcttgat atgcttggag atggagattc tcctggacct ggatttactc ctcatgattc
     2461 tgctccttat ggagcacttg atatggctga ttttgaattt gaacaaatgt ttactgatgc
     2521 tcttggaatt gatgaatatg gaggagatcc ttctgctgga gatatgagag ctgctaatct
     2581 ttggccttct cctcttatga ttaagaggtc taagaagaac tctcttgctc tttctcttac
     2641 tgctgaccaa atggtttctg ctcttcttga tgctgaacct cctattcttt attctgaata
     2701 tgatcctact agaccttttt ctgaggcttc tatgatggga cttcttacta atcttgctga
     2761 tagagaactt gttcatatga ttaattgggc taagagagtt cctggatttg ttgatcttac
     2821 tcttcatgac caagttcatc ttcttgaatg tgcttggctt gaaattctta tgattggact
     2881 tgtttggagg tctatggaac atcctgttaa acttcttttt gctcctaatc ttcttcttga
     2941 tagaaatcaa ggaaagtgtg ttgaaggaat ggttgaaatt tttgatatgc ttcttgctac
     3001 ttcttcaaga tttagaatga tgaatcttca aggagaagaa tttgtttgtc ttaagtctat
     3061 tattcttctt aattctggag tttatacttt tctttcttct actcttaagt ctcttgaaga
     3121 aaaggatcat attcatagag ttcttgataa gattactgat actcttattc atcttatggc
     3181 taaggctgga cttactcttc aacaacaaca tcaaagactt gctcaacttc ttcttattct
     3241 ttctcatatt agacatatgt ctaataaggg aatggaacat ctttattcta tgaagtgtaa
     3301 gaatgttgtt cctctttatg atcttcttct tgaaatgctt gatgctcata gacttcatgc
     3361 tcctacttca agaggaggag cttctgttga agaaactgac caatctcatc ttgctactgc
     3421 tggatctact tcttctcatt ctcttcaaaa gtattatatt actggagaag ctgaaggatt
     3481 tcctgctact gtttgagctt tgagaccctg
//
