LOCUS       sequence-31496-e         910 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RAB4B
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     268..287
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     371..390
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        371..389
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    390..414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             429..451
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             470..487
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             497..514
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
       61 tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
      121 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa
      181 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
      241 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
      301 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat
      361 cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta
      421 gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca
      481 tcatcacagc agcggcctgg ttccgcgtgg tagtatttgg tcggattttc tgttcaaatt
      541 cctggtgatc ggcagcgcgg gaacgggtaa gtcttgctta ttgcaccagt ttattgagaa
      601 taaattcaaa caagactcca accacaccat cggggttgag tttggcagtc gggtcgtaaa
      661 tgtgggtgga aagactgtta aactgcagat atgggatacg gccggccaag aaagatttcg
      721 atcagtcaca cgttcgtact atcgcggtgc agctggggcg cttctcgtgt acgacattac
      781 tagccgtgaa acatataact ctttagccgc atggctgacc gatgctcgca cgttggcgag
      841 tcccaatatc gtagttattc tttgtggaaa caaaaaggat ctggacccgg aacgggaggt
      901 gaccttcctc
//
