LOCUS       sequence-314979-       13214 bp    DNA     circular SYN 14-APR-2025
DEFINITION  For Flp-mediated cassette exchange. Expresses tdTomato in the 
            presence of (r)tTA, switching to connective tissue growth factor in 
            the presence of Cre and (r)tTA activity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13214)
  AUTHORS   Ahmadzadeh E, Bayin NS, Qu X, Singh A, Madisen L, Stephen D, Zeng H,
            Joyner AL, Rosello-Diez A
  TITLE     A collection of genetic mouse lines and related tools for inducible 
            and reversible intersectional mis-expression.
  JOURNAL   Development. 2020 May 28;147(10). pii: dev.186650. doi: 
            10.1242/dev.186650.
  PUBMED    32366677
REFERENCE   2  (bases 1 to 13214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2020-05-28"; volume: "147(10). pii: 
            dev.186650. doi"; pages: " 10.1242/dev.186650"
COMMENT     Sequence Label: pDragon-Ctgf
FEATURES             Location/Qualifiers
     source          1..13214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3009..3323
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    3017..3035
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3053..3071
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3088..3106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3124..3142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3160..3178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3195..3213
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3231..3249
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3296..3320
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(3333..3366)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3376..3385
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3515..3533)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3783..3802
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3977..4000
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    4813..4861
                     /note="synthetic polyadenylation signal"
     polyA_signal    4870..5346
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5069..5088)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     protein_bind    complement(5350..5383)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6462..7050
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6515..6535)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6933..6944)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7075..7092)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7081..7288
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    9786..9819
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        9846..10345
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9881..9904)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10278..10297
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(10993..11009)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10993..11009)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11006..11028)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11017..11033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11041..11047,11048..11065,11066..11071))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11086..11107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11224..11241)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11395..11983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11475..11494)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12154..12945,12946..13014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12777..12796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13015..13119)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13187..13205
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgaagttc
      421 ctattcttca aatagtatag gaacttcttt cggccggcca atcgattgga tccgttttat
      481 gccgcggtta ccgtgaagat atcttaatta agaattcgag ctcgctagag ccccatcctc
      541 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt
      601 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc
      661 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac
      721 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag
      781 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc
      841 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc
      901 tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt
      961 ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat
     1021 cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa
     1081 cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc
     1141 cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc
     1201 ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat
     1261 atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg
     1321 ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc
     1381 tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc
     1441 tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa
     1501 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     1561 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     1621 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     1681 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagcg
     1741 agctcggtac ccagagcccc atcctcactg actccgtcct ggagttggat gagagataat
     1801 ggccttacgt tgtgccaggg gagggtcggg ctggatttag caagatttac cttctccaaa
     1861 gagcggtgct gcagtggcac agctgcccac ggaggtgggg gggtcaccgt ccctggaggt
     1921 gatgaagaac tgtggggatg tggcactgag ggacatggcc agtgggcacg gtgggtgggt
     1981 tggggttggt cttggggatc ttggagggct tttccagcct tcatgatttg acgattgtat
     2041 gaacatctac atggcaattc tccagctgcc tgtcccagtc ctactgaccc agctgtatct
     2101 ctccaggcaa gctcttccac cccttctgct tgcatccaga caccatcaaa catgcaggct
     2161 cagacacagg gaccagcagt gtctgtggcc tttttgtgct cctctccatg ctgggtttta
     2221 acttgctctt tgtccttcta tcctatcttc ttatccttaa ggctgttctg aacgctgtga
     2281 cttggagagt gtcccagagc cctcaacacc tgcatgtccc acgtccatgc tgtcctgcac
     2341 ttccttatcc ccaagatctg cctctccgtg atgcactgaa ttggcaaaca tgtgtcaccc
     2401 cagaccaaca atgtcacagc aaactccccc ttgataggac aagggggaat ggctttacac
     2461 tgagacaggg gaggtttggg ttggatatga ggaggcagtt tttcccccag agggtggtga
     2521 cgcactgaac aggttgccca aggaggctgt ggatgcccca tccctgcagg cattcaaggc
     2581 caggctggat gtggctctgg gcagcctggg ctgctggttg atgaccctgc acatagcagg
     2641 gggttggatc tggatgagca ctgtgctcct ttgcaaccca ggccgttcta tgattctgtc
     2701 attctaaatc tctctttcag cctaaagctt tttccccgta tccccccagg tgtctgcagg
     2761 ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc
     2821 gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag
     2881 ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc
     2941 tttggggggg ggctgtccct ctaggggtac ccggggatcc accggtatcg attacctatc
     3001 acaaattcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     3061 agtgatagag aacgatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
     3121 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     3181 gtatgtcgag tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
     3241 tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc
     3301 gtttagtgaa ccgtcagatc gcctatttaa atataacttc gtatagcata cattatacga
     3361 agttattcgc gagccgccac catggtgagc aagggcgagg aggtcatcaa agagttcatg
     3421 cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag
     3481 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
     3541 cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac
     3601 gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag
     3661 tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc
     3721 ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac
     3781 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc
     3841 cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac
     3901 tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac
     3961 tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     4021 cagtacgagc gctccgaggg ccgccaccac ctgttcctgg ggcatggtac cggcagcacc
     4081 ggcagcggca gctccggcac cgcctcctcc gaggacaaca acatggccgt gattaaggag
     4141 tttatgaggt ttaaagtcag gatggagggc agcatgaacg ggcacgagtt tgagattgaa
     4201 ggagaggggg aagggaggcc ctatgagggc acccagacag caaagctgaa agtgacaaag
     4261 ggcggccctc tgccattcgc atgggacatc ctgtctcctc agtttatgta tggaagtaaa
     4321 gcctacgtga aacaccctgc tgacattcca gattacaaga aactgagctt cccagagggg
     4381 tttaagtggg aaagagtgat gaacttcgaa gacgggggcc tggtgactgt cacccaggac
     4441 agctctctgc aggacggcac tctgatctat aaggtgaaaa tgcgcggcac caattttccc
     4501 cctgatggcc cagtcatgca gaagaagaca atgggctggg aggcttcaac agaaaggctg
     4561 taccccagag acggcgtgct gaagggagag atccaccagg cactgaagct gaaagatgga
     4621 gggcattatc tggtcgaatt caaaaccatc tacatggcca agaaacccgt gcagctgcct
     4681 ggctactatt acgtcgacac aaagctggat attactagcc ataacgaaga ctacaccatt
     4741 gtggagcagt atgagcggag cgaggggagg caccatctgt ttctgtacgg catggacgag
     4801 ctgtacaagt aaaataaaat atctttattt tcattccatc tgtgtgttgg ttttttgtgt
     4861 gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     4921 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     4981 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     5041 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     5101 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcaggct
     5161 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     5221 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     5281 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     5341 gtccttctga taacttcgta tagcatacat tatacgaagt tatcacgcgt gccaccatgc
     5401 tcgcctccgt cgcaggtccc atcagcctcg ccttggtgct cctcgccctc tgcacccggc
     5461 ctgctacggg ccaggactgc agcgcgcaat gtcagtgcgc agccgaagca gcgccgcact
     5521 gccccgccgg cgtgagcctg gtgctggacg gctgcggctg ctgccgcgtc tgcgccaagc
     5581 agctgggaga actgtgtacg gagcgtgacc cctgcgaccc acacaagggc ctcttctgcg
     5641 atttcggctc ccccgccaac cgcaagattg gagtgtgcac tgccaaagat ggtgcaccct
     5701 gtgtcttcgg tgggtcggtg taccgcagcg gtgagtcctt ccaaagcagc tgcaaatacc
     5761 aatgcacttg cctggatggg gccgtgggct gcgtgcccct atgcagcatg gacgtgcgcc
     5821 tgcccagccc tgactgcccc ttcccgagaa gggtcaagct gcctgggaaa tgctgcgagg
     5881 agtgggtgtg tgacgagccc aaggaccgca cagcagttgg ccctgcccta gctgcctacc
     5941 gactggaaga cacatttggc ccagacccaa ctatgatgcg agccaactgc ctggtccaga
     6001 ccacagagtg gagcgcctgt tctaagacct gtggaatggg catctccacc cgagttacca
     6061 atgacaatac cttctgcaga ctggagaagc agagccgcct ctgcatggtc aggccctgcg
     6121 aagctgacct ggaggaaaac attaagaagg gcaaaaagtg catccggaca cctaaaatcg
     6181 ccaagcctgt caagtttgag ctttctggct gcaccagtgt gaagacatac agggctaagt
     6241 tctgcggggt gtgcacagac ggccgctgct gcacaccgca cagaaccacc actctgccag
     6301 tggagttcaa atgccccgat ggcgagatca tgaaaaagaa tatgatgttc atcaagacct
     6361 gtgcctgcca ttacaactgt cctggggaca atgacatctt tgagtccctg tactacagga
     6421 agatgtacgg agacatggcg taatacgtaa agcttgtcga taatcaacct ctggattaca
     6481 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6541 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6601 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6661 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     6721 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     6781 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6841 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctatgtt gccacctgga
     6901 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6961 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7021 gtcggatctc cctttgggcc gcctccccgc atcgacaccg agcgctgctc gaggcctcga
     7081 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     7141 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     7201 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     7261 gggaagacaa tagcaggcat gctggggaca cgtgggatcc tctagagccc catcctcact
     7321 gactccgtcc tggagttgga tgagagataa tggccttacg ttgtgccagg ggagggtcgg
     7381 gctggattta gcaagattta ccttctccaa agagcggtgc tgcagtggca cagctgccca
     7441 cggaggtggg ggggtcaccg tccctggagg tgatgaagaa ctgtggggat gtggcactga
     7501 gggacatggc cagtgggcac ggtgggtggg ttggggttgg tcttggggat cttggagggc
     7561 ttttccagcc ttcatgattt gacgattgta tgaacatcta catggcaatt ctccagctgc
     7621 ctgtcccagt cctactgacc cagctgtatc tctccaggca agctcttcca ccccttctgc
     7681 ttgcatccag acaccatcaa acatgcaggc tcagacacag ggaccagcag tgtctgtggc
     7741 ctttttgtgc tcctctccat gctgggtttt aacttgctct ttgtccttct atcctatctt
     7801 cttatcctta aggctgttct gaacgctgtg acttggagag tgtcccagag ccctcaacac
     7861 ctgcatgtcc cacgtccatg ctgtcctgca cttccttatc cccaagatct gcctctccgt
     7921 gatgcactga attggcaaac atgtgtcacc ccagaccaac aatgtcacag caaactcccc
     7981 cttgatagga caagggggaa tggctttaca ctgagacagg ggaggtttgg gttggatatg
     8041 aggaggcagt ttttccccca gagggtggtg acgcactgaa caggttgccc aaggaggctg
     8101 tggatgcccc atccctgcag gcattcaagg ccaggctgga tgtggctctg ggcagcctgg
     8161 gctgctggtt gatgaccctg cacatagcag ggggttggat ctggatgagc actgtgctcc
     8221 tttgcaaccc aggccgttct atgattctgt cattctaaat ctctctttca gcctaaagct
     8281 ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg
     8341 aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg
     8401 gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc
     8461 gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc tctagagccc
     8521 catcctcact gactccgtcc tggagttgga tgagagataa tggccttacg ttgtgccagg
     8581 ggagggtcgg gctggattta gcaagattta ccttctccaa agagcggtgc tgcagtggca
     8641 cagctgccca cggaggtggg ggggtcaccg tccctggagg tgatgaagaa ctgtggggat
     8701 gtggcactga gggacatggc cagtgggcac ggtgggtggg ttggggttgg tcttggggat
     8761 cttggagggc ttttccagcc ttcatgattt gacgattgta tgaacatcta catggcaatt
     8821 ctccagctgc ctgtcccagt cctactgacc cagctgtatc tctccaggca agctcttcca
     8881 ccccttctgc ttgcatccag acaccatcaa acatgcaggc tcagacacag ggaccagcag
     8941 tgtctgtggc ctttttgtgc tcctctccat gctgggtttt aacttgctct ttgtccttct
     9001 atcctatctt cttatcctta aggctgttct gaacgctgtg acttggagag tgtcccagag
     9061 ccctcaacac ctgcatgtcc cacgtccatg ctgtcctgca cttccttatc cccaagatct
     9121 gcctctccgt gatgcactga attggcaaac atgtgtcacc ccagaccaac aatgtcacag
     9181 caaactcccc cttgatagga caagggggaa tggctttaca ctgagacagg ggaggtttgg
     9241 gttggatatg aggaggcagt ttttccccca gagggtggtg acgcactgaa caggttgccc
     9301 aaggaggctg tggatgcccc atccctgcag gcattcaagg ccaggctgga tgtggctctg
     9361 ggcagcctgg gctgctggtt gatgaccctg cacatagcag ggggttggat ctggatgagc
     9421 actgtgctcc tttgcaaccc aggccgttct atgattctgt cattctaaat ctctctttca
     9481 gcctaaagct ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag
     9541 cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg
     9601 ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct
     9661 gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc
     9721 tctagagtcg acggcgcgcc gtaaaagctt ggcatagatc ttattgcaga agccatatgt
     9781 tttaggtgcc agggcgtgcc cttgggctcc ccgggcgcgt ttgggcggcc gcatcgaatt
     9841 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     9901 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     9961 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
    10021 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
    10081 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
    10141 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
    10201 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
    10261 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
    10321 ctcttcctca tctccgggcc tttcgacctg caatcgccgc tagccgccac catgaaaaag
    10381 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
    10441 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
    10501 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
    10561 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
    10621 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
    10681 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
    10741 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
    10801 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
    10861 actgtgatgg acgacaccgt cagtgtaagt gctagcccct aggcctgaat cgaagttcct
    10921 attcttcaaa aggtatagga acttcgagct cgtcgactgc agaggcctgc atgcaagctt
    10981 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    11041 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    11101 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    11161 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    11221 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    11281 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    11341 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    11401 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    11461 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    11521 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    11581 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    11641 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    11701 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    11761 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    11821 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    11881 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    11941 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    12001 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    12061 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    12121 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    12181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    12241 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    12301 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    12361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    12421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    12481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    12541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    12601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    12661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    12721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    12781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    12841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    12901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    12961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    13021 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    13081 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    13141 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    13201 gaggcccttt cgtc
//
