LOCUS       sequence-315032-       10345 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10345)
  AUTHORS   Luo JH, Fu ZY, Losi G, Kim BG, Prybylowski K, Vissel B, Vicini S
  TITLE     Functional expression of distinct NMDA channel subunits tagged with 
            green fluorescent protein in hippocampal neurons in culture.
  JOURNAL   Neuropharmacology. 2002 Mar . 42(3):306-18.
  PUBMED    11897109
REFERENCE   2  (bases 1 to 10345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuropharmacology. 2002 Mar . 42(3):306-18."
COMMENT     Sequence Label: pEGFP-NR1a
FEATURES             Location/Qualifiers
     source          1..10345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1037..1039,1040..1042,1043..1753)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1037..1039,1040..1042,1043..1753)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1082..1103)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1343..1362)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1690..1711
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5702..5719)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5708..5932
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5978..6406
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6065..6084)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6275..6296
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6415..6435)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6420..6749
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6600..6735
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6662..6681
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6816..7610
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6870..6889)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7480..7499
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7784..7905
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7821..7840)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7875..7894
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7954..7970)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7954..7970)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7967..7989)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7978..7994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8002..8008,8009..8026,8027..8032))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8047..8068
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8185..8202)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8356..8944)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8436..8455)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9115..9906,9907..9975))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9738..9757
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9976..10080)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10155..10174)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttgatatcga attccgttgc tgtcgaatat tggtttgtta
      721 aggcagtttc tgtagaggtt tctaagagac cagtcgcgca gtccgcgctg ctgtcctttc
      781 cgccttttcc gcgcgggtgt tcgagcagcg ccaaacacgc ttcagcacct cggacagcat
      841 ccgccgcgct cgcccggggc tcctagagaa cccgggggcg cttggccgcg cgcgggcggc
      901 ccgcgggtcg tacatcgcga ggtcgtcgca ctcgcgcaac ccagagccag gcccgctgtg
      961 cccggagctc atgagcacca tgcacctgct gacattcgcc ctgctttttt cctgctcctt
     1021 cgcccgcgcg gatcccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     1081 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     1141 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     1201 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     1261 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     1321 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     1381 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     1441 caacatcttg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     1501 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     1561 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     1621 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     1681 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     1741 cgagctgtac aaggatcccg accccaagat cgtcaacatc ggcgcggtgc tgagcacgcg
     1801 taagcatgaa cagatgttcc gcgaggcagt aaaccaggcc aataagcgac acggctcttg
     1861 gaagatacag ctcaacgcca cttctgtcac ccacaagccc aacgccatac agatggccct
     1921 gtcagtgtgt gaggacctca tctctagcca ggtctacgct atcctagtta gccacccgcc
     1981 tactcccaac gaccacttca ctcccacccc tgtctcctac acagctggct tctacagaat
     2041 ccctgtcctg ggactgacta cccgaatgtc catctactct gacaagagta tccacctgag
     2101 tttccttcgc acggtgccgc cctactccca ccagtccagc gtctggtttg agatgatgcg
     2161 agtctacaac tggaaccaca tcatcctgct ggtcagcgac gaccacgagg gacgggcagc
     2221 gcagaagcgc ttggagacgt tgctggagga acgggagtcc aaggcagaga aggtgctgca
     2281 gtttgaccca ggaaccaaga atgtgacggc tctgctgatg gaggcccggg aactggaggc
     2341 ccgggtcatc atcctttctg caagcgagga cgacgctgcc acagtgtacc gcgcagccgc
     2401 aatgctgaac atgacgggct ctgggtacgt gtggctggtc ggggaacgcg agatctctgg
     2461 gaacgccctg cgctacgctc ctgatggcat catcggactt cagctcatca atggcaagaa
     2521 tgagtcagcc cacatcagtg acgccgtggg cgtggtggca caggcagttc acgaactcct
     2581 agagaaggag aatatcactg acccaccgcg gggttgcgtg ggcaacacca acatctggaa
     2641 gacaggacca ttgttcaaga gggtgctgat gtcttctaag tatgcggacg gagtgactgg
     2701 ccgtgtggaa ttcaatgagg atggggaccg gaagtttgcc aactatagta tcatgaacct
     2761 gcagaaccgc aagctggtgc aagtgggcat ctacaatggt acccatgtca tcccaaatga
     2821 caggaagatc atctggccag gaggagagac agagaaacct cgaggatacc agatgtccac
     2881 cagactaaag atagtgacaa tccaccaaga gcccttcgtg tacgtcaagc ccacaatgag
     2941 tgatgggaca tgcaaagagg agttcacagt caatggtgac ccagtgaaga aggtgatctg
     3001 tacggggcct aatgacacgt ccccaggcag cccacgccac acagtgcccc agtgctgcta
     3061 tggcttctgc atagacctgc tcatcaagct ggcgcggacc atgaatttta cctatgaggt
     3121 gcacctggtg gcagatggca agtttggcac acaggagcgg gtaaacaaca gcaacaaaaa
     3181 ggagtggaac ggaatgatgg gcgagctact cagtggccaa gcggacatga ttgtggcacc
     3241 actgaccatc aacaatgagc gtgcgcagta catagagttc tccaagccct tcaagtacca
     3301 gggcctgacc attttggtca agaaggagat tcccaggagc acactggact catttatgca
     3361 gccttttcag agcacactgt ggttgctagt aggactgtca gttcatgtgg tggctgtgat
     3421 gctgtacctg ctggaccgct tcagtccctt tggccgattc aaggtgaaca gtgaggagga
     3481 ggaggaagat gcactgaccc tgtcctctgc catgtggttt tcctggggcg tcctgctcaa
     3541 ctccggcatt ggggaaggtg ccccccggag tttctctgca cgtatcctag gcatggtgtg
     3601 ggctggtttc gccatgatca tagtggcttc ctacactgcc aacttggcag ctttcctggt
     3661 gctggatcgg cctgaggagc gcatcacggg catcaatgac cccaggctca gaaacccctc
     3721 agacaagttc atctacgcaa ctgtaaagca gagctccgtg gacatctact tccggaggca
     3781 ggtggagttg agtaccatgt accggcacat ggaaaaacac aattacgaga gcgcagctga
     3841 ggccatccag gctgtgcggg acaacaagct gcacgccttt atctgggact cggccgtgct
     3901 ggagtttgag gcttcacaga agtgcgatct ggtgaccacg ggtgagctgt tcttccgctc
     3961 aggctttggc atcggcatgc gcaaggacag cccctggaag cagaacgttt ccctgtccat
     4021 actcaagtcc catgagaatg gcttcatgga agatctggat aagacatggg ttcggtatca
     4081 ggaatgcgac tcccgcagca atgctcctgc aaccctcact tttgagaaca tggcaggggt
     4141 cttcatgctg gtggctggag gcatcgtagc tgggattttc ctcattttca ttgagatcgc
     4201 ctacaagcga cacaaggatg cccgtaggaa gcagatgcag ctggcttttg cagccgtgaa
     4261 cgtgtggagg aagaacctgc aggatagaaa gagtggtaga gcagagcccg accctaaaaa
     4321 gaaagccaca tttagggcta tcacctccac cctggcctcc agcttcaaga gacgtaggtc
     4381 ctccaaagac acgagcaccg ggggtggacg cggcgctttg caaaaccaaa aagacacagt
     4441 gctgccgcga cgcgctattg agagggagga gggccagctg cagctgtgtt cccgtcatag
     4501 ggagagctga gacgccccgc ccgccctcct ctgcccctcc cccgcagaca gacgcacggg
     4561 acagcggcct ggcccacgca gagccccgga gcacgacggg gtcgggggag gagcactccc
     4621 agcctccccc aggccgtgcc cgcctgccca ccggtcggcc ggctggccgg tccaccctgt
     4681 cccggccccg cgcgtgcccc cgacgtcgga gctaacgggc cgccttgtct gtgtatttct
     4741 attttacagc agtaccatcc cactgatatc acgggcccgc tcaacctctc agatccctcg
     4801 gtcagcaccg tggtgtgagg ccccccggag gcgcccacct gcccagttag cccggccaag
     4861 gacactgatg agtcctgctg ctcgggaagg cctgagggaa gcccacccgc cccagagact
     4921 gcccaccctg ggcctcccgt ccgcctgctc tgctgcctgg cgggcagccc ctgcaggacc
     4981 aaggtgcgga ccagagcggc tgaggatggg ccagagctga gccggctggg cagggccaca
     5041 gggcgctccg gcagaggcag ggccctgagg tctctgagca gtggggtgag gggcctaagt
     5101 ggccccggtc ggaggagtct ggagcagaaa tggcagcccc atccttcctc cagccactac
     5161 cccaagctac agtgggggcc tatggcccca gcttgctagg tcacccccga cccttcctcc
     5221 agcgcctgct ctctgcaact tgatttccac ctctctcctg ctgcaccacc ctcccacgac
     5281 atttccccac cccattcact gggttgtctc tgacctttcc cagggctagc cttcactgcc
     5341 ctagtggcag tgcttcaggg gtgctttctg gctcccagac atctagggct ccagactcca
     5401 agagggctga gccttctctt ctgtccgcag ccacaatagg cttcctcaga cgctggctcg
     5461 tgatgagtcc cgcaccttgg gcaccaggga gcgccatctg cctcccagtc cggtgtcact
     5521 caccccacta ccttgtacat gaccagctct cccagtgtcc cagtgtctgc cccagggaca
     5581 ccgggcgcgc acagccaccc ctaatcccgg tattcagtgg tgatgcctaa aggaatgtca
     5641 gnaaaaaaaa aaaaaaagcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca
     5701 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5761 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5821 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5881 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     5941 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6001 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6061 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6121 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6181 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6241 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6301 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6361 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     6421 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6481 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6541 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     6601 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6661 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     6721 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     6781 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     6841 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     6901 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     6961 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     7021 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     7081 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     7141 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     7201 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     7261 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     7321 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     7381 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     7441 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     7501 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     7561 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     7621 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     7681 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     7741 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     7801 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7861 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     7921 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7981 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8041 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8101 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8161 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8221 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8281 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8341 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8401 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8461 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8521 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8581 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8641 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8701 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8761 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8821 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8881 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8941 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9001 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9061 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9121 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9181 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9241 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9301 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9361 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9421 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9481 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9541 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9601 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9661 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9721 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9781 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9841 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9901 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9961 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10021 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10081 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
    10141 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
    10201 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
    10261 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
    10321 gatgtacggg ccagatatac gcgtt
//
