LOCUS       sequence-31505-e         720 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ARL10B
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..93
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             630..647
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(700..718)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 agaataattt tgtttacttt tagaaggaga tataccatgg gcagcagcca tcatcatcat
       61 catcacagca gcggcctggt tccgcgtggt agtaaggagg agatggagct cacgctggtc
      121 gggcttcagt actcgggcaa gaccaccttc gtcaacgtga tcgcgtcagg acagttcaac
      181 gaggacatga tccccaccgt gggtttcaac atgcgcaaaa tcaccaaagg gaatgtgact
      241 atcaagctct gggacattgg gggacagccg cgtttccgca gcatgtggga gcgctactgc
      301 cgaggagtga gcgccatcgt gtacatggtg gatgctgctg accaggagaa gattgaggcc
      361 tctaagaacg agctccacaa cctactggac aaacctcagc tgcagggcat cccggtctta
      421 gtcctgggta acaagcgaga ccttccggga gcattggatg agaaggagct gattgagaaa
      481 atgaatctgt ctgccatcca ggaccgagag atctgctgct actccatctc ttgcaaagaa
      541 aaggacaaca ttgacatcac cctacagtgg cttattcaac actcgaagtc acggagaagc
      601 tgatgacgaa gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg
      661 ctaacaaagc ccgaaaggaa nctgagttgg ctgctgccac cgctgagcaa taactagcat
//
