LOCUS       sequence-315457-        5165 bp    DNA     circular SYN 15-APR-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0621:Dendra
FEATURES             Location/Qualifiers
     source          1..5165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1109..1132
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1389..2189
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2314..2902
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2803..2822
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgtcccagat catgtacaac tacccggcga tgctcgcgca
      121 cgccgcggag atgaacacct actccggtgc gctgcacgcc gtcggcgccg acatcgccgc
      181 cgagcagcac gcgctggcga gcgcctggca gggcgacacc ggtatgacct accaggcgtg
      241 gcaggcccag tggaaccagg ccatggagga actcgtgcgt gcctaccgcg cgatggccac
      301 cacgcacgag cagaacacca tggcgatgag cgcgcgcgac caggccgaag gcgccaagtg
      361 gggaaagctt ggtgcggccg cgggcaacac gcctggcatc aatctgatca aggaggacat
      421 gcgtgtcaag gtgcatatgg agggcaacgt gaacggtcac gccttcgtga tcgaggggga
      481 gggtaagggt aagccttacg aagggaccca gaccgccaac ctcaccgtca aggagggcgc
      541 ccctctccca ttctcgtacg atatcctcac taccgcggtg cattacggga accgcgtgtt
      601 caccaagtac cccgaggata tccctgacta cttcaagcag agcttcccgg agggatactc
      661 ctgggagcgg acgatgacct tcgaagacaa gggtatctgt acgatccgca gcgacatctc
      721 gctggaaggg gactgtttct tccagaacgt ccgtttcaag gggaccaact ttccgccgaa
      781 cggcccggtc atgcagaaga agaccctcaa gtgggagccg agcacggaga agctgcacgt
      841 tcgggacggc ctcctggtcg gcaacatcaa catggcgctg ctgctggaag gcggcggcca
      901 ctacctatgc gacttcaaga ccacgtacaa agcgaagaag gtcgtccagc tgcccgacgc
      961 gcacttcgtg gaccaccgca tcgaaatcct ggggaacgat tcggactaca acaaggtcaa
     1021 gttgtacgag cacgctgtgg cacgctatag cccgctcccg tcgcaggtct ggttcgaagg
     1081 cggcggcggg gagaacctgt atttccagga ctacaaggac gacgacgaca agtgatttaa
     1141 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgttaacta gcgtacgatc
     1201 gaattcctgc agctcacggt aactgatgcc gtatttgcag taccagcgta cggcccacag
     1261 aatgatgtca cgctgaaaat gccggccttt gaatgggttc atgtgcagct ccatcagcaa
     1321 aaggggatga taagtttatc accaccgact atttgcaaca gtgccgttga tcgtgctatg
     1381 atcgactgat gtcatcagcg gtggagtgca atgtcgtgca atacgaatgg cgaaaagccg
     1441 agctcatcgg tcagcttctc aaccttgggg ttacccccgg cggtgtgctg ctggtccaca
     1501 gctccttccg tagcgtccgg cccctcgaag atgggccact tggactgatc gaggccctgc
     1561 gtgctacgct gggtccggga gggacgctcg tcatgccctc gtggtcaggt ctggacgacg
     1621 agccgttcga tcctgccacg tcgcccgtta caccggacct tggagttgtc tctgacacat
     1681 tctggcgcct gccaaatgta aagcgcagcg cccatccatt tgcctttgcg gcagcggggc
     1741 cacaggcaga gcagatcatc tctgatccat tgcccctgcc accttactcg cctgcaagcc
     1801 cggtcgcccg tgtccatgaa ctcgatgggc aggtacttct cctcggcgtg ggacacgatg
     1861 ccaacacgac gctgcatctt gccgagttga tggcaaaggt tccctatggg gtgccgagac
     1921 actgcaccat tcttcaggat ggcaagttgg tacgcgtcga ttatctcgag aatgaccact
     1981 gctgtgagcg ctttgccttg gcggacaggt ggctcaagga gaagagcctt cagaaggaag
     2041 gtccagtcgg tcatgccttt gctcggttga tccgctcccg cgacattgtg gcgacagccc
     2101 tgggtcaact gggccgagat ccgttgatct tcctgcatcc gccagaggcg ggatgcgaag
     2161 aatgcgatgc cgctcgccag tcgattggct gagctcatga gcggagaacg agatgacgtt
     2221 ggaggggcaa ggtcgcgctg attgctgggg caacacgggg gatccactag ttccatgagc
     2281 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2341 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2401 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2461 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2521 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2581 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2641 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2701 tgagctatga gaaagcgcca cgcttcccga ggggagaaag gcggacaggt atccggtaag
     2761 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2821 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2881 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2941 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3001 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc
     3061 gtgcggccgc ggtacccggg gatcctctag agtcgaccac caagggcacc atctctgctt
     3121 gggccacccc gttggccgca gccagctcgc tgagagccgt gaacgacagg gcgaacgcca
     3181 gcccgccgac ggcgagggtt ccgaccgctg caactcccgg tgcaaccttg tcccggtcta
     3241 ttctcttcac tgcaccagct ccaatctggt gtgaatgccc ctcgtctgtt cgcgcaggcg
     3301 gggggctcta ttcgtttgtc agcatcgaaa gtagccagat cagggatgcg ttgcaaccgc
     3361 gtatgcccag gtcagaagag tcgcacaaga gttgcagacc cctggaaaga aaaatggcca
     3421 gagggcgaaa acaccctctg accagcggag cgggcgacgg gaatcgaacc cgcgtagcta
     3481 gtttggaaga atgggtgtct gccgaccaca tatgggccgg tcaagatagg tttttacccc
     3541 ctctcggctg catcctctaa gtggaaagaa attgcaggtc gtagaagcgc gttgaagcct
     3601 gagagttgca caggagttgc aacccggtag ccttgttcac gacgagagga gacctagttg
     3661 gcacgtcgcg gatggggatc gctgaagact cagcgcagcg ggaggatcca agcctcatac
     3721 gtcaacccgc aggacggtgt gaggtactac gcgctgcaga cctacgacaa caagatggac
     3781 gccgaagcct ggctcgcggg cgagaagcgg ctcatcgaga tggagacctg gacccctcca
     3841 caggaccggg cgaagaaggc agccgccagc gccatcacgc tggaggagta cacccggaag
     3901 tggctcgtgg agcgcgacct cgcagacggc accagggatc tgtacagcgg gcacgcggag
     3961 cgccgcatct acccggtgct aggtgaagtg gcggtcacag agatgacgcc agctctggtg
     4021 cgtgcgtggt gggccgggat gggtaggaag cacccgactg cccgccggca tgcctacaac
     4081 gtcctccggg cggtgatgaa cacagcggtc gaggacaagc tgatcgcaga gaacccgtgc
     4141 cggatcgagc agaaggcagc cgatgagcgc gacgtagagg cgctgacgcc tgaggagctg
     4201 gacatcgtcg ccgctgagat cttcgagcac taccggatcg cggcatacat cctggcgtgg
     4261 acgagcctcc ggttcggaga gctgatcgag cttcgccgca aggacatcgt ggacgacggc
     4321 atgacgatga agctccgggt gcgccgtggc gcttcccgcg tggggaacaa gatcgtcgtt
     4381 ggcaacgcca agaccgtccg gtcgaagcgt cctgtgacgg ttccgcctca cgtcgcggag
     4441 atgatccgag cgcacatgaa ggaccgtacg aagatgaaca agggccccga ggcattcctg
     4501 gtgaccacga cgcagggcaa ccggctgtcg aagtccgcgt tcaccaagtc gctgaagcgt
     4561 ggctacgcca agatcggtcg gccggaactc cgcatccacg acctccgcgc tgtcggcgct
     4621 acgttcgccg ctcaggcagg tgcgacgacc aaggagctga tggcccgtct cggtcacacg
     4681 actcctagga tggcgatgaa gtaccagatg gcgtctgagg cccgcgacga ggctatcgct
     4741 gaggcgatgt ccaagctggc caagacctcc tgaaacgcaa aaagcccccc tcccaaggac
     4801 actgagtcct aaagaggggg gtttcttgtc agtacgcgaa gaaccacgcc tggccgcgag
     4861 cgccagcacc gccgctctgt gcggagacct gggcaccagc cccgccgccg ccaggagcat
     4921 tgccgttccc gccagaaatc tagacggtga ccacaacgcg cccgctttga tcggggacgt
     4981 cgaaatattg gatcgtcggc accgtcacgg ccgtgggagg cggcacgatc cgcgacgtga
     5041 tgatcggccg catccccacg gtgctgcgca gtgagctcta cgccatcccg gcgttgatct
     5101 gtgcgttcgc acgcacaggc ccggtgtgag aagggtctct gacgagcggg agaactccct
     5161 atcag
//
