LOCUS       sequence-315463-        6050 bp    DNA     circular SYN 15-APR-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0711:Dendra
FEATURES             Location/Qualifiers
     source          1..6050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1994..2017
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2274..3074
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3199..3787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3688..3707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggcccaacg cgagtgggtc gagaaggatt tctacaaaga
      121 actcggcgtc tcctctgacg ccagcgccga cgagatcaag aaggcctatc ggaaactggc
      181 cgccgagctg caccctgacc ggaactccga tcctggagcc gcggagcggt tcaaggcggt
      241 gtccgaggcg aacagtgttc tgtcggaccc cgctaagcgc aaggagtatg acgagacccg
      301 gcggttgttc gcaggtggtt tcggtcgcag gttcaaccag ggcggcggcg gattcggagg
      361 cggtttcggt tccgacggag tcgaattcaa cctcggcgac ctgttcgacg ctgccgggca
      421 gaccggtggc gcgaacatcg gcgacctctt cggtggactg ttcggccgcg gcccccagca
      481 gcggccgagc cgtccgcgtc gcggcaacga cctggagacc gagaccgaac tgtcgttcct
      541 ggaggccacc aagggcgtgg ccatgccgct gcggctgacc agcccggcgc cgtgcaccaa
      601 ctgccacggc agcggtgcac ggccgggcac cagcccgaag gtgtgcccga gctgcaacgg
      661 ctccggtgtg atcagccgca accagggcgc tttcggtttc tccgaaccct gcaccgagtg
      721 tcgtggtagc ggctcgatca tcgagcatcc ctgccaggaa tgccggggca ccggcgtcac
      781 caccaggacc cgcaccatca acgtgcggat tccccccggt gtcgaggacg gtcagcgcat
      841 ccggctggcc gggcagggcg aggcgggcct gcgcggcgcg ccgtccggtg acctctacgt
      901 caccgtgcac gtgcgtcccg acaaggtgtt cgggcgcgac ggcgacgacc tgaccgtcac
      961 ggttcccgtg agtttccacg aactggcgct cgggacaacg ctttcggtcc ccacactcga
     1021 cggcaaggtc ggagttcggg tgcccaaggg cacctcggac ggccggatcc tccgcgtccg
     1081 cggacgcggt gtgcccaagc ggtcgggtgg ccacggggat ctactggtca ccgtgaaggt
     1141 cgcggtgcca ccgaacctgg aaggtgaagc ggcagaagcg ctggaggctt acgcgaaagc
     1201 tgagcgtgcc agcggattcg atccgcgtgc tggatgggcg gggaatgtaa agcttggtgc
     1261 ggccgcgggc aacacgcctg gcatcaatct gatcaaggag gacatgcgtg tcaaggtgca
     1321 tatggagggc aacgtgaacg gtcacgcctt cgtgatcgag ggggagggta agggtaagcc
     1381 ttacgaaggg acccagaccg ccaacctcac cgtcaaggag ggcgcccctc tcccattctc
     1441 gtacgatatc ctcactaccg cggtgcatta cgggaaccgc gtgttcacca agtaccccga
     1501 ggatatccct gactacttca agcagagctt cccggaggga tactcctggg agcggacgat
     1561 gaccttcgaa gacaagggta tctgtacgat ccgcagcgac atctcgctgg aaggggactg
     1621 tttcttccag aacgtccgtt tcaaggggac caactttccg ccgaacggcc cggtcatgca
     1681 gaagaagacc ctcaagtggg agccgagcac ggagaagctg cacgttcggg acggcctcct
     1741 ggtcggcaac atcaacatgg cgctgctgct ggaaggcggc ggccactacc tatgcgactt
     1801 caagaccacg tacaaagcga agaaggtcgt ccagctgccc gacgcgcact tcgtggacca
     1861 ccgcatcgaa atcctgggga acgattcgga ctacaacaag gtcaagttgt acgagcacgc
     1921 tgtggcacgc tatagcccgc tcccgtcgca ggtctggttc gaaggcggcg gcggggagaa
     1981 cctgtatttc caggactaca aggacgacga cgacaagtga tttaaataac cccttggggc
     2041 ctctaaacgg gtcttgaggg gttttttgtt aactagcgta cgatcgaatt cctgcagctc
     2101 acggtaactg atgccgtatt tgcagtacca gcgtacggcc cacagaatga tgtcacgctg
     2161 aaaatgccgg cctttgaatg ggttcatgtg cagctccatc agcaaaaggg gatgataagt
     2221 ttatcaccac cgactatttg caacagtgcc gttgatcgtg ctatgatcga ctgatgtcat
     2281 cagcggtgga gtgcaatgtc gtgcaatacg aatggcgaaa agccgagctc atcggtcagc
     2341 ttctcaacct tggggttacc cccggcggtg tgctgctggt ccacagctcc ttccgtagcg
     2401 tccggcccct cgaagatggg ccacttggac tgatcgaggc cctgcgtgct acgctgggtc
     2461 cgggagggac gctcgtcatg ccctcgtggt caggtctgga cgacgagccg ttcgatcctg
     2521 ccacgtcgcc cgttacaccg gaccttggag ttgtctctga cacattctgg cgcctgccaa
     2581 atgtaaagcg cagcgcccat ccatttgcct ttgcggcagc ggggccacag gcagagcaga
     2641 tcatctctga tccattgccc ctgccacctt actcgcctgc aagcccggtc gcccgtgtcc
     2701 atgaactcga tgggcaggta cttctcctcg gcgtgggaca cgatgccaac acgacgctgc
     2761 atcttgccga gttgatggca aaggttccct atggggtgcc gagacactgc accattcttc
     2821 aggatggcaa gttggtacgc gtcgattatc tcgagaatga ccactgctgt gagcgctttg
     2881 ccttggcgga caggtggctc aaggagaaga gccttcagaa ggaaggtcca gtcggtcatg
     2941 cctttgctcg gttgatccgc tcccgcgaca ttgtggcgac agccctgggt caactgggcc
     3001 gagatccgtt gatcttcctg catccgccag aggcgggatg cgaagaatgc gatgccgctc
     3061 gccagtcgat tggctgagct catgagcgga gaacgagatg acgttggagg ggcaaggtcg
     3121 cgctgattgc tggggcaaca cgggggatcc actagttcca tgagcgtcag accccgtaga
     3181 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3241 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3301 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     3361 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3421 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3481 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3541 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3601 cgccacgctt cccgagggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3661 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3721 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3781 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3841 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3901 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc aacgcgtgcg gccgcggtac
     3961 ccggggatcc tctagagtcg accaccaagg gcaccatctc tgcttgggcc accccgttgg
     4021 ccgcagccag ctcgctgaga gccgtgaacg acagggcgaa cgccagcccg ccgacggcga
     4081 gggttccgac cgctgcaact cccggtgcaa ccttgtcccg gtctattctc ttcactgcac
     4141 cagctccaat ctggtgtgaa tgcccctcgt ctgttcgcgc aggcgggggg ctctattcgt
     4201 ttgtcagcat cgaaagtagc cagatcaggg atgcgttgca accgcgtatg cccaggtcag
     4261 aagagtcgca caagagttgc agacccctgg aaagaaaaat ggccagaggg cgaaaacacc
     4321 ctctgaccag cggagcgggc gacgggaatc gaacccgcgt agctagtttg gaagaatggg
     4381 tgtctgccga ccacatatgg gccggtcaag ataggttttt accccctctc ggctgcatcc
     4441 tctaagtgga aagaaattgc aggtcgtaga agcgcgttga agcctgagag ttgcacagga
     4501 gttgcaaccc ggtagccttg ttcacgacga gaggagacct agttggcacg tcgcggatgg
     4561 ggatcgctga agactcagcg cagcgggagg atccaagcct catacgtcaa cccgcaggac
     4621 ggtgtgaggt actacgcgct gcagacctac gacaacaaga tggacgccga agcctggctc
     4681 gcgggcgaga agcggctcat cgagatggag acctggaccc ctccacagga ccgggcgaag
     4741 aaggcagccg ccagcgccat cacgctggag gagtacaccc ggaagtggct cgtggagcgc
     4801 gacctcgcag acggcaccag ggatctgtac agcgggcacg cggagcgccg catctacccg
     4861 gtgctaggtg aagtggcggt cacagagatg acgccagctc tggtgcgtgc gtggtgggcc
     4921 gggatgggta ggaagcaccc gactgcccgc cggcatgcct acaacgtcct ccgggcggtg
     4981 atgaacacag cggtcgagga caagctgatc gcagagaacc cgtgccggat cgagcagaag
     5041 gcagccgatg agcgcgacgt agaggcgctg acgcctgagg agctggacat cgtcgccgct
     5101 gagatcttcg agcactaccg gatcgcggca tacatcctgg cgtggacgag cctccggttc
     5161 ggagagctga tcgagcttcg ccgcaaggac atcgtggacg acggcatgac gatgaagctc
     5221 cgggtgcgcc gtggcgcttc ccgcgtgggg aacaagatcg tcgttggcaa cgccaagacc
     5281 gtccggtcga agcgtcctgt gacggttccg cctcacgtcg cggagatgat ccgagcgcac
     5341 atgaaggacc gtacgaagat gaacaagggc cccgaggcat tcctggtgac cacgacgcag
     5401 ggcaaccggc tgtcgaagtc cgcgttcacc aagtcgctga agcgtggcta cgccaagatc
     5461 ggtcggccgg aactccgcat ccacgacctc cgcgctgtcg gcgctacgtt cgccgctcag
     5521 gcaggtgcga cgaccaagga gctgatggcc cgtctcggtc acacgactcc taggatggcg
     5581 atgaagtacc agatggcgtc tgaggcccgc gacgaggcta tcgctgaggc gatgtccaag
     5641 ctggccaaga cctcctgaaa cgcaaaaagc ccccctccca aggacactga gtcctaaaga
     5701 ggggggtttc ttgtcagtac gcgaagaacc acgcctggcc gcgagcgcca gcaccgccgc
     5761 tctgtgcgga gacctgggca ccagccccgc cgccgccagg agcattgccg ttcccgccag
     5821 aaatctagac ggtgaccaca acgcgcccgc tttgatcggg gacgtcgaaa tattggatcg
     5881 tcggcaccgt cacggccgtg ggaggcggca cgatccgcga cgtgatgatc ggccgcatcc
     5941 ccacggtgct gcgcagtgag ctctacgcca tcccggcgtt gatctgtgcg ttcgcacgca
     6001 caggcccggt gtgagaaggg tctctgacga gcgggagaac tccctatcag
//
