LOCUS       sequence-31554-e         858 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Pf-GUK1
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     276..295
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     379..398
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        379..397
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    398..422
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             437..459
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             478..495
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             505..522
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccttgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt
       61 cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc
      121 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg
      181 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
      241 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
      301 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag
      361 atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt
      421 cccctctaga aataattttg tttaacttta agaaggagat ataccatggg cagcagccat
      481 catcatcatc atcacagcag cggcctggtt ccgcgtggta gtatgaataa tatttatccc
      541 ctggttattt gtggtccttc gggtgttggt aaaggtacct tgataaaaaa gctgttgaat
      601 gaatttccaa attattttta tttttccgta agctgtacga caagaaaaaa gagagagaaa
      661 gaaaaagaag gtgtagatta ttatttcata gataagacta tatttgaaga taaattaaaa
      721 aatgaagatt ttttagaata tgataattat gctaataatt tttatggaac attaaaaagt
      781 gaatatgata aagcaaaaga acaaaataaa atctgcttat ttgaaatgaa tattaatggt
      841 gtcaaacaat tgaaaaaa
//
