LOCUS       sequence-315661-        5294 bp    DNA     circular SYN 15-APR-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0754:Dendra
FEATURES             Location/Qualifiers
     source          1..5294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1238..1261
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1518..2318
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2443..3031
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2932..2951
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgacacggat gggtgatctg ctcggaccag acccggttta
      121 ccttccaggc gatcctgcgg ccgaagagga actggcggca ggcgagaagg cagcggtggt
      181 tgctgcggct cacccgtcgg cctcggtcgc gtgggcgacg ctcgccgagc aggcgttgga
      241 cgacgacaag gcggtcaccg catacgcgta cgcgcggacc ggctaccacc gtgggctgga
      301 tcagttgcgc cgcaacgggt ggaagggctt cggccccgtg ccgttcagcc acgagccaaa
      361 ccagggtttc ctgcggtgcg tggcggcgct ggcgcgagcg gccgacgaca tcggcgagac
      421 cgacgagtac cagcgctgcc tggacctgct cgacgactgc gatccgacgg cgcgtcgcga
      481 actcggtctg ggcaagcttg gtgcggccgc gggcaacacg cctggcatca atctgatcaa
      541 ggaggacatg cgtgtcaagg tgcatatgga gggcaacgtg aacggtcacg ccttcgtgat
      601 cgagggggag ggtaagggta agccttacga agggacccag accgccaacc tcaccgtcaa
      661 ggagggcgcc cctctcccat tctcgtacga tatcctcact accgcggtgc attacgggaa
      721 ccgcgtgttc accaagtacc ccgaggatat ccctgactac ttcaagcaga gcttcccgga
      781 gggatactcc tgggagcgga cgatgacctt cgaagacaag ggtatctgta cgatccgcag
      841 cgacatctcg ctggaagggg actgtttctt ccagaacgtc cgtttcaagg ggaccaactt
      901 tccgccgaac ggcccggtca tgcagaagaa gaccctcaag tgggagccga gcacggagaa
      961 gctgcacgtt cgggacggcc tcctggtcgg caacatcaac atggcgctgc tgctggaagg
     1021 cggcggccac tacctatgcg acttcaagac cacgtacaaa gcgaagaagg tcgtccagct
     1081 gcccgacgcg cacttcgtgg accaccgcat cgaaatcctg gggaacgatt cggactacaa
     1141 caaggtcaag ttgtacgagc acgctgtggc acgctatagc ccgctcccgt cgcaggtctg
     1201 gttcgaaggc ggcggcgggg agaacctgta tttccaggac tacaaggacg acgacgacaa
     1261 gtgatttaaa taaccccttg gggcctctaa acgggtcttg aggggttttt tgttaactag
     1321 cgtacgatcg aattcctgca gctcacggta actgatgccg tatttgcagt accagcgtac
     1381 ggcccacaga atgatgtcac gctgaaaatg ccggcctttg aatgggttca tgtgcagctc
     1441 catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag tgccgttgat
     1501 cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc
     1561 gaaaagccga gctcatcggt cagcttctca accttggggt tacccccggc ggtgtgctgc
     1621 tggtccacag ctccttccgt agcgtccggc ccctcgaaga tgggccactt ggactgatcg
     1681 aggccctgcg tgctacgctg ggtccgggag ggacgctcgt catgccctcg tggtcaggtc
     1741 tggacgacga gccgttcgat cctgccacgt cgcccgttac accggacctt ggagttgtct
     1801 ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg
     1861 cagcggggcc acaggcagag cagatcatct ctgatccatt gcccctgcca ccttactcgc
     1921 ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg
     1981 gacacgatgc caacacgacg ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg
     2041 tgccgagaca ctgcaccatt cttcaggatg gcaagttggt acgcgtcgat tatctcgaga
     2101 atgaccactg ctgtgagcgc tttgccttgg cggacaggtg gctcaaggag aagagccttc
     2161 agaaggaagg tccagtcggt catgcctttg ctcggttgat ccgctcccgc gacattgtgg
     2221 cgacagccct gggtcaactg ggccgagatc cgttgatctt cctgcatccg ccagaggcgg
     2281 gatgcgaaga atgcgatgcc gctcgccagt cgattggctg agctcatgag cggagaacga
     2341 gatgacgttg gaggggcaag gtcgcgctga ttgctggggc aacacggggg atccactagt
     2401 tccatgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2461 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2521 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2581 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2641 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2701 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2761 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2821 cctacagcgt gagctatgag aaagcgccac gcttcccgag gggagaaagg cggacaggta
     2881 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2941 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3001 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3061 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3121 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3181 gcgcaacgcg tgcggccgcg gtacccgggg atcctctaga gtcgaccacc aagggcacca
     3241 tctctgcttg ggccaccccg ttggccgcag ccagctcgct gagagccgtg aacgacaggg
     3301 cgaacgccag cccgccgacg gcgagggttc cgaccgctgc aactcccggt gcaaccttgt
     3361 cccggtctat tctcttcact gcaccagctc caatctggtg tgaatgcccc tcgtctgttc
     3421 gcgcaggcgg ggggctctat tcgtttgtca gcatcgaaag tagccagatc agggatgcgt
     3481 tgcaaccgcg tatgcccagg tcagaagagt cgcacaagag ttgcagaccc ctggaaagaa
     3541 aaatggccag agggcgaaaa caccctctga ccagcggagc gggcgacggg aatcgaaccc
     3601 gcgtagctag tttggaagaa tgggtgtctg ccgaccacat atgggccggt caagataggt
     3661 ttttaccccc tctcggctgc atcctctaag tggaaagaaa ttgcaggtcg tagaagcgcg
     3721 ttgaagcctg agagttgcac aggagttgca acccggtagc cttgttcacg acgagaggag
     3781 acctagttgg cacgtcgcgg atggggatcg ctgaagactc agcgcagcgg gaggatccaa
     3841 gcctcatacg tcaacccgca ggacggtgtg aggtactacg cgctgcagac ctacgacaac
     3901 aagatggacg ccgaagcctg gctcgcgggc gagaagcggc tcatcgagat ggagacctgg
     3961 acccctccac aggaccgggc gaagaaggca gccgccagcg ccatcacgct ggaggagtac
     4021 acccggaagt ggctcgtgga gcgcgacctc gcagacggca ccagggatct gtacagcggg
     4081 cacgcggagc gccgcatcta cccggtgcta ggtgaagtgg cggtcacaga gatgacgcca
     4141 gctctggtgc gtgcgtggtg ggccgggatg ggtaggaagc acccgactgc ccgccggcat
     4201 gcctacaacg tcctccgggc ggtgatgaac acagcggtcg aggacaagct gatcgcagag
     4261 aacccgtgcc ggatcgagca gaaggcagcc gatgagcgcg acgtagaggc gctgacgcct
     4321 gaggagctgg acatcgtcgc cgctgagatc ttcgagcact accggatcgc ggcatacatc
     4381 ctggcgtgga cgagcctccg gttcggagag ctgatcgagc ttcgccgcaa ggacatcgtg
     4441 gacgacggca tgacgatgaa gctccgggtg cgccgtggcg cttcccgcgt ggggaacaag
     4501 atcgtcgttg gcaacgccaa gaccgtccgg tcgaagcgtc ctgtgacggt tccgcctcac
     4561 gtcgcggaga tgatccgagc gcacatgaag gaccgtacga agatgaacaa gggccccgag
     4621 gcattcctgg tgaccacgac gcagggcaac cggctgtcga agtccgcgtt caccaagtcg
     4681 ctgaagcgtg gctacgccaa gatcggtcgg ccggaactcc gcatccacga cctccgcgct
     4741 gtcggcgcta cgttcgccgc tcaggcaggt gcgacgacca aggagctgat ggcccgtctc
     4801 ggtcacacga ctcctaggat ggcgatgaag taccagatgg cgtctgaggc ccgcgacgag
     4861 gctatcgctg aggcgatgtc caagctggcc aagacctcct gaaacgcaaa aagcccccct
     4921 cccaaggaca ctgagtccta aagagggggg tttcttgtca gtacgcgaag aaccacgcct
     4981 ggccgcgagc gccagcaccg ccgctctgtg cggagacctg ggcaccagcc ccgccgccgc
     5041 caggagcatt gccgttcccg ccagaaatct agacggtgac cacaacgcgc ccgctttgat
     5101 cggggacgtc gaaatattgg atcgtcggca ccgtcacggc cgtgggaggc ggcacgatcc
     5161 gcgacgtgat gatcggccgc atccccacgg tgctgcgcag tgagctctac gccatcccgg
     5221 cgttgatctg tgcgttcgca cgcacaggcc cggtgtgaga agggtctctg acgagcggga
     5281 gaactcccta tcag
//
