LOCUS       sequence-315818-        9106 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express Dox repressible NFS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9106)
  AUTHORS   Sviderskiy VO, Blumenberg L, Gorodetsky E, Karakousi TR, Hirsh N, 
            Alvarez SW, Terzi EM, Kaparos E, Whiten GC, Ssebyala S, Tonzi P, Mir
            H, Neel BG, Huang TT, Adams S, Ruggles KV, Possemato R
  TITLE     Hyperactive CDK2 Activity in Basal-like Breast Cancer Imposes a 
            Genome Integrity Liability that Can Be Exploited by Targeting DNA 
            Polymerase epsilon.
  JOURNAL   Mol Cell. 2020 Oct 28. pii: S1097-2765(20)30723-1. doi: 
            10.1016/j.molcel.2020.10.016.
  PUBMED    33152268
REFERENCE   2  (bases 1 to 9106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2020 Oct 28. pii: S1097-2765(20)30723-1. doi: 
            10.1016/j.molcel.2020.10.016."
COMMENT     Sequence Label: pCW57.1 DOX off blast NFS1
FEATURES             Location/Qualifiers
     source          1..9106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..315
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2032..2542
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2461..2479
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2552..2944
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             2954..3007
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3008..3625,3626..3631,3632..3667,3668..3670,3671..3706,
                     3707..3709,3710..3745,3746..3754)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3794..3814)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4212..4223)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4428..4661
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4733..4854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4770..4789)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4824..4843
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4894..5029
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5029..5100
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5101..5169,5170..5961)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5319..5338)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6132..6720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6621..6640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6874..6891
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7008..7029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7044..7049,7050..7067,7068..7074)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7082..7098
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7087..7109
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7106..7122
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7106..7122
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7141..7161
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7143..7161
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7189..7415
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7416..7596
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7643..7768
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8261..8494
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8679..8723
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8936..9053
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcctggag aattggctag catgctgctc cgagccgctt ggaggcgggc
      361 ggcagtggcg gtgacagcgg ctccagggcc gaagcccgcg gcgcccactc gggggctgcg
      421 cctgcgcgtt ggagaccgtg ctcctcagtc tgcggttccc gcagatacag ccgctgcccc
      481 ggaggtgggg ccagtgctgc gacctctcta tatggatgtg caagctacaa ctcctctgga
      541 cccccgggtg cttgatgcca tgctccctta cctaattaac tactatggga acccacactc
      601 ccggacacat gcttatggct gggagagtga ggcagccatg gaacgtgctc gtcagcaagt
      661 agcatctctg attggagctg atcctcgtga gatcattttt actagtggtg ctactgaatc
      721 caacaacata gcaattaagg gggtggcccg attctacagg tcacggaaaa agcacttgat
      781 caccacccag acagaacaca aatgtgtctt ggactcctgc cgttcactgg aagctgaggg
      841 ctttcaggtc acctacctcc cagtgcagaa gagtgggatc attgacctaa aggaactaga
      901 ggctgctatc cagccagata ctagcctggt gtcagtcatg actgtgaaca atgagattgg
      961 agtgaagcag cctattgcag aaatagggcg gatttgttca agtcgtaaag tttacttcca
     1021 tactgatgca gcccaggctg ttggaaaaat cccacttgat gtcaatgaca tgaaaattga
     1081 tctcatgagc attagtggtc acaaaatcta cggtcccaaa ggggttggtg ccatctacat
     1141 ccgtcgccgg ccccgtgtgc gtgtggaggc cctgcagagt ggaggggggc aggagcgggg
     1201 tatgcggtct gggacagtgc ccacaccctt agtggtgggg ctgggggctg cgtgtgaggt
     1261 ggcacagcaa gagatggagt atgaccacaa gcgaatctca aagttgtcag agcggctgat
     1321 acagaatata atgaagagcc ttccagatgt ggtgatgaat ggggacccta agcaccatta
     1381 tcccggctgt atcaacctct cctttgcata tgtggaaggg gaaagtctgc tgatggcact
     1441 gaaggacgtt gccttatcct cagggagtgc ctgcacctct gcatccctgg agccctctta
     1501 tgtgcttaga gcaattggca ctgatgagga tttagcgcac tcttctatca ggtttggaat
     1561 tggccgcttc actacagagg aggaagtgga ctacacagtg gagaaatgca ttcagcatgt
     1621 gaagcgtctt cgagaaatga gccctctctg ggagatggtt caggatggca ttgacctcaa
     1681 gagcatcaag tggacccaac actaggtcga ccgttcagct gcttatgctg ctcgttgggt
     1741 ggcaaaatcc cttgttaaag gaggtctgtg ccggagagtt cttgttcagg tctcttatgc
     1801 tattggagtt tctcatccat tatctatctc cattttccat tatggtacct ctcagaagag
     1861 tgagagagag ctattagaga ttgtgaagaa gaatttcgat ctccgccctg gggtcattgt
     1921 cagggatctg gatctgaaga agccaattta tcagaggact gcagcctatg gccactttgg
     1981 tagggacagc ttcccatggg aagtgcccaa aaagcttaaa tattgaggtc cggggttggg
     2041 gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg
     2101 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag
     2161 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc
     2221 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc
     2281 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc
     2341 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga
     2401 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg
     2461 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg
     2521 aatcaccgac ctctctcccc agcaattcac catggttcct ttgtctcaag aagaatccac
     2581 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     2641 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     2701 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     2761 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     2821 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     2881 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     2941 gggccccggt gccgaaggta gaggttctct cctcacttgt ggtgatgttg aagaaaaccc
     3001 tggtccaatg tctagactgg acaagagcaa agtcataaac tctgctctgg aattactcaa
     3061 tgaagtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca
     3121 gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tggcaatcga
     3181 gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt
     3241 tctgcggaac aacgccaagt cattccgctg tgctctcctc tcacatcgcg acggggctaa
     3301 agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc
     3361 gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg
     3421 ccactttaca ctgggctgcg tattggagga tcaggagcat caagtagcaa aagaggaaag
     3481 agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga
     3541 ccatcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga
     3601 gaaacagcta aagtgcgaaa gcggcgggcc ggccgacgcc cttgacgatt ttgacttaga
     3661 catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct
     3721 tgacgatttt gaccttgaca tgctccccgg gtaactaagt aaggatcgat ccaagatatc
     3781 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     3841 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     3901 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     3961 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4021 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4081 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     4141 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4201 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4261 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4321 cctccccgcc tgtttcgcct cggcgtccgg actagaggta cctttaagac caatgactta
     4381 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     4441 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     4501 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4561 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4621 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     4681 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     4741 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     4801 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     4861 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4921 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4981 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct ttcaaatatg
     5041 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5101 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5161 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5221 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5281 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5341 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5401 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5461 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5521 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5581 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5641 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5701 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5761 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5821 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5881 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5941 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6001 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6061 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6121 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6181 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6241 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6301 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6361 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6421 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6481 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6541 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6601 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6661 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6721 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6781 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6841 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6901 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     6961 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     7021 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     7081 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     7141 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     7201 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     7261 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     7321 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     7381 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     7441 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7501 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7561 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     7621 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     7681 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     7741 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     7801 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     7861 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     7921 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     7981 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     8041 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     8101 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     8161 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     8221 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     8281 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     8341 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     8401 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     8461 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     8521 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     8581 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     8641 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     8701 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     8761 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     8821 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     8881 caaccccgag gggacaattc tcgacctcga gacaaatggc agtattcatc cacaatttta
     8941 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     9001 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     9061 attacaggga cagcagagat ccactttggc cgcgaatcga tatgtc
//
