LOCUS       sequence-315969-       12378 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gene targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12378)
  AUTHORS   Yoshimatsu S, Ohtsu K, Sato T, Yamamoto M, Sasaki E, Shiozawa S, 
            Okano H
  TITLE     Generation and validation of a common marmoset embryonic stem cell 
            line ActiCre-B1 that ubiquitously expresses a tamoxifen-inducible 
            Cre-driver.
  JOURNAL   Stem Cell Res. 2021 Jan 8;51:102164. doi: 10.1016/j.scr.2021.102164.
  PUBMED    33453576
REFERENCE   2  (bases 1 to 12378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res."; date: "2021-01-8"; volume: "51:102164. doi"; pages: " 
            10.1016/j.scr.2021.102164"
COMMENT     Sequence Label: pKI-cjACTB-IRES-ERT2CreERT2-IRES-Hyg
FEATURES             Location/Qualifiers
     source          1..12378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     239..256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    1860..2433
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     regulatory      2443..2452
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2452..3387
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             3397..4419
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             4432..5367
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4480..5373
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     misc_feature    join(5413..5987,5988..5990,5991..5999)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6025..7044
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             6025..7044
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     primer_bind     complement(9266..9285)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9267..9285)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9292..9309)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9292..9308)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9301..9323)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             9446..9748
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9627..9646
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             10097..10891
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10097..10891
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10151..10170)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10761..10780
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      11091..11100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11097..11471
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      11609..12197
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12098..12117
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12351..12368
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcagatctg
      301 gcaccacact ttttacaacg agctgcgtgt ggctcctgag gagcaccccg tgctgcttac
      361 tgaggccccc ctgaacccca aggccaaccg tgagaagatg acccaggtga gtgatctgcc
      421 acctcccctg gtggccgcct ccctccttcc tggcctcccg gagctgcgcc ctttctcact
      481 ggttctctct tctgccgttc tccgtaggac tctcttctct gacctgagtc tcctttggaa
      541 ctctgcaggt tctatttgct ttttcccaga tgaggtcttt ctggtgtttg cctttctgac
      601 taggtgtcta aaacagacag tgctgtgggt gtaggtacta acactggctc gtgtgacaag
      661 cccatgaggc tggtgtaaag cagtcttgga gtgtgtatac agtaggcgca cagtaggtct
      721 gaacagaatc cccatcccaa gaccccagca caactagcgg tgttcttgca cttactgcat
      781 gtcccctgtc tgctcttggc tgtccctagt gacttcctca gtgtgacacg gtgcatctct
      841 ttctacagat catgtttgag accttcaaca ccccagccat gtatgtggcc atccaggctg
      901 tgctttcctt gtacgcctct ggccgtacca ctggcatcgt gatggactcc ggtgacgggg
      961 tcacccacac tgtgcccatc tatgaggggt atgccctccc ccacgccatc ctacgtctgg
     1021 acctggctgg ccgggacctg actgactacc tcatgaagat cctcaccgag cgcggctaca
     1081 gcttcaccac cacagctgag cgggaaatcg tgcgtgacat caaggagaag ctgtgctacg
     1141 tcgccctgga cttcgagcag gagatggcca cggcggcctc cagctcctcc ctggagaaga
     1201 gctatgagct gcccgatggt caggtcatca ccatcggcaa cgagcggttc cgctgccctg
     1261 aggcactctt ccagccttct ttcctgggta agtggagacc actgcctagc cctgcctgac
     1321 atgaggttac cttggggctg tgctgtggaa gcttaagtct gggtctcatt tccccctcag
     1381 gcatggaatc ttgtggcatc cacgaaacta cctttaactc catcatgaaa tgtgacgtgg
     1441 acatccgcaa agacctgtat gccaacacag tgctgtctgg tggtaccacc atgtaccctg
     1501 gcatcgctga caggatgcag aaggagatca ccgccctggc acccagcaca atgaagatca
     1561 aggtgggtgt ccttccagcc tgagctgacc tgggcagggg gcatggggcc ctgtggtttg
     1621 tggggagctg tcatatcaag ggtcctcact gcctgttccc tccactcctc agatcattgc
     1681 tcctcccgag cgcaagtact cagtgtggat tggtggctcc atcctggcct cgctgtccac
     1741 cttccagcag atgtggatca gcaagcagga gtatgatgag tccggcccct ccatcgtcca
     1801 ccgcaaatgc ttctaggcgg actgtgactt agtgaattcc cttttttttc cacagtccgc
     1861 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     1921 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     1981 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     2041 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     2101 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     2161 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     2221 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     2281 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac
     2341 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     2401 gacgtggttt tcctttgaaa aacacgatga taagggtgag ccgccaccat ggctggagac
     2461 atgagagctg ccaacctttg gccaagcccg ctcatgatca aacgctctaa gaagaacagc
     2521 ctggccttgt ccctgacggc cgaccagatg gtcagtgcct tgttggatgc tgagcccccc
     2581 atactctatt ccgagtatga tcctaccaga cccttcagtg aagcttcgat gatgggctta
     2641 ctgaccaacc tggcagacag ggagctggtt cacatgatca actgggcgaa gagggtgcca
     2701 ggctttgtgg atttgaccct ccatgatcag gtccaccttc tagaatgtgc ctggctagag
     2761 atcctgatga ttggtctcgt ctggcgctcc atggagcacc cagtgaagct actgtttgct
     2821 cctaacttgc tcttggacag gaaccaggga aaatgtgtag agggcatggt ggagatcttc
     2881 gacatgctgc tggctacatc atctcggttc cgcatgatga atctgcaggg agaggagttt
     2941 gtgtgcctca aatctattat tttgcttaat tctggagtgt acacatttct gtccagcacc
     3001 ctgaagtctc tggaagagaa ggaccatatc caccgagtcc tggacaagat cacagacact
     3061 ttgatccacc tgatggccaa ggcaggcctg accctgcagc agcagcacca gcggctggcc
     3121 cagctcctcc tcatcctctc ccacatcagg cacatgagta acaaaggcat ggagcatctg
     3181 tacagcatga agtgcaagaa cgtggtgccc ctctatgacc tgctgctgga ggcggcggac
     3241 gcccaccgcc tacatgcgcc cactagccgt ggaggggcat ccgtggagga gacggaccaa
     3301 agccacttgg ccactgcggg ctctacttca tcgcattcct tgcaaaagta ttacatcacg
     3361 ggggaggcag agggtttccc tgccacagct gtcgacaatt tactgaccgt acaccaaaat
     3421 ttgcctgcat taccggtcga tgcaacgagt gatgaggttc gcaagaacct gatggacatg
     3481 ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg
     3541 tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga acctgaagat
     3601 gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa
     3661 catttgggcc agctaaacat gcttcatcgt cggtccgggc tgccacgacc aagtgacagc
     3721 aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt
     3781 gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc actcatggaa
     3841 aatagcgatc gctgccagga tatacgtaat ctggcatttc tggggattgc ttataacacc
     3901 ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg tactgacggt
     3961 gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag
     4021 aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt ctctggtgta
     4081 gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca
     4141 tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc aactcatcga
     4201 ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc tggacacagt
     4261 gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc ggagatcatg
     4321 caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa cctggatagt
     4381 gaaacagggg caatggtgcg cctgctggaa gatggcgatc tcgagccatc tgctggagac
     4441 atgagagctg ccaacctttg gccaagcccg ctcatgatca aacgctctaa gaagaacagc
     4501 ctggccttgt ccctgacggc cgaccagatg gtcagtgcct tgttggatgc tgagcccccc
     4561 atactctatt ccgagtatga tcctaccaga cccttcagtg aagcttcgat gatgggctta
     4621 ctgaccaacc tggcagacag ggagctggtt cacatgatca actgggcgaa gagggtgcca
     4681 ggctttgtgg atttgaccct ccatgatcag gtccaccttc tagaatgtgc ctggctagag
     4741 atcctgatga ttggtctcgt ctggcgctcc atggagcacc cagtgaagct actgtttgct
     4801 cctaacttgc tcttggacag gaaccaggga aaatgtgtag agggcatggt ggagatcttc
     4861 gacatgctgc tggctacatc atctcggttc cgcatgatga atctgcaggg agaggagttt
     4921 gtgtgcctca aatctattat tttgcttaat tctggagtgt acacatttct gtccagcacc
     4981 ctgaagtctc tggaagagaa ggaccatatc caccgagtcc tggacaagat cacagacact
     5041 ttgatccacc tgatggccaa ggcaggcctg accctgcagc agcagcacca gcggctggcc
     5101 cagctcctcc tcatcctctc ccacatcagg cacatgagta acaaaggcat ggagcatctg
     5161 tacagcatga agtgcaagaa cgtggtgccc ctctatgacc tgctgctgga ggcggcggac
     5221 gcccaccgcc tacatgcgcc cactagccgt ggaggggcat ccgtggagga gacggaccaa
     5281 agccacttgg ccactgcggg ctctacttca tcgcattcct tgcaaaagta ttacatcacg
     5341 ggggaggcag agggtttccc tgccacagct tgatagcggc cgcactagag gaattccgcc
     5401 cctctccccc cccccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
     5461 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     5521 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     5581 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     5641 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     5701 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     5761 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     5821 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     5881 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     5941 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc
     6001 gtaccaaaga tggatagatc cggaaagcct gaactcaccg cgacgtctgt cgagaagttt
     6061 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     6121 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     6181 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     6241 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
     6301 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     6361 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     6421 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     6481 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     6541 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     6601 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     6661 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     6721 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     6781 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     6841 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     6901 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     6961 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     7021 actcgtccga gggcaaagga atagagtaga tgccgaccga acaagagctg atttcgagaa
     7081 cgcctcagcc agcaactcgc gcgagcctag caaggcaaat gcgagagaac ggccttacgc
     7141 ttggtggcac agttctcgtc cacagttcgc taagctcgct cggctgggtc gcgggagggc
     7201 cggtcgcagt gattcaggcc cttctggatt gtgttggtcc ccagggcacg attgtcatgc
     7261 ccacgcactc gggtgatctg actgatcccg cagattggag atcgccgccc gtgcctgccg
     7321 attgggtgca gatctctaga taactgatca taatcagcca taccacattt gtagaggttt
     7381 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     7441 ttgttgttgt tgggcttacc tgtacactga cttgagacca gttgaataaa gtgcacacct
     7501 taaaaatgag gccaagcatg actttgtgtc gggctggggt gtgggcagca ggtgaaccca
     7561 ggggaagggt ggggcagtgg gggccagcct gtccctactg agtggaggtg ggctgaggtt
     7621 ctctctcctt gacattgagc agccttagaa tgtgagggaa gtttaagatt caggcctctg
     7681 ctcattgggg agttcctggc ttggccctgc cacctgtccc taggtacccc agtttctctg
     7741 ggttcatcca gaaggtctag atgctcagga agtgctggtt ggcacctgtg tttcttagga
     7801 gtggagatcc cttagggtca ctccgggcag ggggttcttg agtgggagtg tagtgggtgt
     7861 tcctagctgc catgcctttg tcctgctcac tgtattggag tggagtcgtc cataggctga
     7921 ggagaagcca tgactcctgc ttctctaccc agacagggaa atctgggatc acagtcgaat
     7981 tgtggtgggg gatccatggg gccatgtggt tggcaggcag ctctggttag ctctggactg
     8041 agctggattc tgccttgtga cgagtaggcg ccatcctgtg gcctctcagg gcccagtttg
     8101 tgacccatgc tgcagaggga gatggccaga ctcctggaca gcagggcatg cctggcattg
     8161 caggtcagat tctttttttt ttgagatggg gtctggcttt gttgctcagg ctttagttca
     8221 gtggtacaac cacagctcac tgcagcctca gccttctggg caagcagttc tgcctcagcc
     8281 tcccaaatag ctggaactac acaggcacac ccagctagtt gtgggtcaga ttctcacttg
     8341 tgcagagctg agtcactttt gaaagaaaca aaacctggct tcccttccgc tgctagaagc
     8401 tttgattgat agtagcggag aatgaaggaa ggagacgagg ctcccttgcg gggataccct
     8461 ttaccaaaaa caccacctgg tgtatcacag ttgttctgga aatgatctgg tccccaggag
     8521 gcctggactg cctctcactt gtgcacagtg gctgctggca gagaggaagg ttgtcccttt
     8581 ctgtgttatc ttggaggttc cccgaagggt ctgccaagag cagttctagc tcttttggtc
     8641 tttactgagt gaccactaga tggtgggcct gaagctactg agatgacctg gacaagaacc
     8701 ctgccttgga ggagctgtca atctggaaat gaggacttca ttcatttgtt cattccttga
     8761 tccatttctc cattgaatca ctatttgtgc tatgtactgt gggtatggag gagaaccaaa
     8821 cagatgtgtt cctgtcctca ccaggcaccc aatttaaagg ggaagacatc ataaacacaa
     8881 aggtacacaa atgagtgtcc aatgacacgg tgtcatcatg ggaagaaaaa gcagaggctt
     8941 tgatgaacac gatcattagg tgatattcat tatgttgatt gtggtggtct agacataaat
     9001 taacacttat ctaggctggg cgtggtggct catgcctgta atcccagcac tttgggaggc
     9061 tgaggtgggt ggatcaagag atcgagacca tcctggccaa catagtgaaa ccccatcttt
     9121 actaaaaata caaaaattag ctgggcgtgg tggtgcacac ttgggaggct gaggtaggag
     9181 aactgcttga actggggagg cgaacgttgc agtgagctaa gattgtgcta ctgtactcca
     9241 gcctggtgct agagggccca attcgcccta tagtgagtcg tattacaatt cactggccgt
     9301 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     9361 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     9421 acagttgcgc agcctatacg tacggcagtt taaggtttac acctataaaa gagagagccg
     9481 ttatcgtctg tttgtggatg tacagagtga tattattgac acgccggggc gacggatggt
     9541 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     9601 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     9661 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     9721 cattaacctg atgttctggg gaatataaat gtcaggcatg agattatcaa aaaggatctt
     9781 cacctagatc cttttcacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa
     9841 tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc
     9901 ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc
     9961 ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat
    10021 ggctttcttg ccgccaagga tctgatggcg caggggatca agctctgatc aagagacagg
    10081 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
    10141 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
    10201 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
    10261 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
    10321 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
    10381 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
    10441 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
    10501 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
    10561 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
    10621 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
    10681 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
    10741 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
    10801 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
    10861 cttctatcgc cttcttgacg agttcttctg aattattaac gcttacaatt tcctgatgcg
    10921 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atcaggtggc acttttcggg
    10981 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    11041 tcatgagaca ataaccctga taaatgcttc aataatagca cgtgaggagg gccaccatgg
    11101 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
    11161 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
    11221 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
    11281 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
    11341 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
    11401 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
    11461 agcaggactg acacgtgcta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    11521 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    11581 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    11641 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    11701 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    11761 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    11821 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    11881 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    11941 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    12001 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    12061 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    12121 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    12181 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    12241 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    12301 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    12361 tgagcgagga agcggaag
//
