LOCUS       sequence-31606-e         907 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UEV1
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             541..558
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(611..629)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      625..672
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(796..815)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggttccgc gtggtagtac aggagtaaaa gtccctcgca atttccgact
      121 gttggaagaa ctcgaagaag gccagaaagg agtaggagat ggcacagtta gctggggtct
      181 agaagatgac gaagacatga cacttacaag atggacaggg atgataattg ggcctccaag
      241 aacaatttat gaaaaccgaa tatacagcct taaaatagaa tgtggaccta aatacccaga
      301 agcacccccc tttgtaagat ttgtaacaaa aattaatatg aatggagtaa atagttctaa
      361 tggagtggtg gacccaagag ccatatcagt gctagcaaaa tggcagaatt catatagcat
      421 caaagttgtc ctgcaagagc ttcggcgcct aatgatgtct aaagaaaata tgaaactccc
      481 tcagccgccc gaaggacagt gttacagcaa ttgatgacga agcttgcggc cgcactcgag
      541 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg
      601 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
      661 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta
      721 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
      781 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
      841 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
      901 acctcga
//
