LOCUS       sequence-316205-        3054 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses LightR-iCre-miRFP670 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Shaaya M, Fauser J, Zhurikhina A, Conage-Pough JE, Huyot V, Brennan 
            M, Flower CT, Matsche J, Khan S, Natarajan V, Rehman J, Kota P, 
            White FM, Tsygankov D, Karginov AV
  TITLE     Light-Regulated allosteric switch enables temporal and subcellular 
            control of enzyme activity.
  JOURNAL   Elife. 2020 Sep 23;9. pii: 60647. doi: 10.7554/eLife.60647.
  PUBMED    32965214
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-09-23"; volume: "9. pii: 60647. doi"; pages: " 
            10.7554/eLife.60647"
COMMENT     Sequence Label: LightR-iCre-miRFP670
FEATURES             Location/Qualifiers
     source          1..3054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..27
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1018..1467
                     /codon_start=1
                     /product="""positive Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=pMag
                     /note="rapidly heterodimerizes with nMagHigh1 upon blue 
                     light irradiation to form a complex with slow switch-off 
                     kinetics"
                     /translation="HTLYAPGGYDIMGYLIQIMNRPNPQVELGPVDTSCALILCDLKQK
                     DTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE"
     regulatory      2104..2113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2110..3054
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
ORIGIN
        1 atggtgccca agaagaagag gaaagtctcc aacctgctga ctgtgcacca aaacctgcct
       61 gccctccctg tggatgccac ctctgatgaa gtcaggaaga acctgatgga catgttcagg
      121 gacaggcagg ccttctctga acacacctgg aagatgctcc tgtctgtgtg cagatcctgg
      181 gctgcctggt gcaagctgaa caacaggaaa tggttccctg ctgaacctga ggatgtgagg
      241 gactacctcc tgtacctgca agccagaggc ctggctgtga agaccatcca acagcacctg
      301 ggccagctca acatgctgca caggagatct ggcctgcctc gcccttctga ctccaatgct
      361 gtgtccctgg tgatgaggag aatcagaaag gagaatgtgg atgctgggga gagagccaag
      421 caggccctgg cctttgaacg cactgacttt gaccaagtca gatccctgat ggagaactct
      481 ggaccaggtg gcagcggagg tcataccttg tatgcgccgg ggggttatga catcatgggt
      541 tacctcatac agatcatgaa taggccgaac ccacaagtgg agctcggacc cgtcgatacc
      601 tcctgcgctc tcattctgtg tgaccttaag cagaaggata cccctatcgt gtacgcctcc
      661 gaggcatttc tgtacatgac agggtactcg aacgccgaag tgctgggacg gaactgccgc
      721 ttcctgcaaa gcccggatgg aatggtgaag cctaagtcaa cccgcaaata cgtggactcc
      781 aacactatca acaccatgcg caaggccatt gaccgcaatg ctgaggtgca agtggaagtg
      841 gtgaacttca agaagaatgg acagcgcttc gtcaacttcc tgactatgat ccccgtgcgc
      901 gacgagaccg gcgaataccg gtacagcatg gggtttcagt gtgagacaga gggcggctcc
      961 ggaggcagcg gcggttctgg aggttccggt ggcggctccg gaggtagcgg agggtctcac
     1021 actctttacg cccctggagg atacgacatt atgggatatt tgattcagat tatgaaccgc
     1081 ccaaaccctc aggtcgaact ggggcctgtg gacacgtcat gtgccctgat cctgtgcgat
     1141 ctgaagcaaa aggacactcc gattgtctac gcctcggaag ccttcttgta tatgaccgga
     1201 tacagcaatg cagaggtgct cggtaggaac tgcagattcc tgcagtcccc cgacgggatg
     1261 gtgaaaccaa agtcgactcg caaatatgtg gactcgaaca cgatcaatac aatgcggaag
     1321 gccatcgacc ggaacgccga ggtccaggtg gaggtggtca actttaagaa gaacggccag
     1381 cggttcgtga actttctcac catgattccg gtccgggatg aaaccggaga gtacagatac
     1441 tccatgggat tccagtgcga aaccgaaggg tccggaggtc ccggaggtag atgccaggac
     1501 atcaggaacc tggccttcct gggcattgcc tacaacaccc tgctgcgcat tgccgaaatt
     1561 gccagaatca gagtgaagga catctcccgc accgatggtg ggagaatgct gatccacatt
     1621 ggcaggacca agaccctggt gtccacagct ggtgtggaga aggccctgtc cctgggggtt
     1681 accaagctgg tggagagatg gatctctgtg tctggtgtgg ctgatgaccc caacaactac
     1741 ctgttctgcc gggtcagaaa gaatggtgtg gctgcccctt ctgccacctc ccaactgtcc
     1801 acccgggccc tggaagggat ctttgaggcc acccaccgcc tgatctatgg tgccaaggat
     1861 gactctgggc agagatacct ggcctggtct ggccactctg ccagagtggg tgctgccagg
     1921 gacatggcca gggctggtgt gtccatccct gaaatcatgc aggctggtgg ctggaccaat
     1981 gtgaacattg tgatgaacta catcagaaac ctggactctg agactggggc catggtgagg
     2041 ctgctcgagg atggggacag aattctgcag tcgacggtac cgcgggcccg ggatccaccg
     2101 gtcgccacca tggtagcagg tcatgcctct ggcagccccg cattcgggac cgcctctcat
     2161 tcgaattgcg aacatgaaga gatccacctc gccggctcga tccagccgca tggcgcgctt
     2221 ctggtcgtca gcgaacatga tcatcgcgtc atccaggcca gcgccaacgc cgcggaattt
     2281 ctgaatctcg gaagcgtact cggcgttccg ctcgccgaga tcgacggcga tctgttgatc
     2341 aagatcctgc cgcatctcga tcccaccgcc gaaggcatgc cggtcgcggt gcgctgccgg
     2401 atcggcaatc cctctacgga gtactgcggt ctgatgcatc ggcctccgga aggcgggctg
     2461 atcatcgaac tcgaacgtgc cggcccgtcg atcgatctgt caggcacgct ggcgccggcg
     2521 ctggagcgga tccgcacggc gggttcactg cgcgcgctgt gcgatgacac cgtgctgctg
     2581 tttcagcagt gcaccggcta cgaccgggtg atggtgtatc gtttcgatga gcaaggccac
     2641 ggcctggtat tctccgagtg ccatgtgcct gggctcgaat cctatttcgg caaccgctat
     2701 ccgtcgtcga ctgtcccgca gatggcgcgg cagctgtacg tgcggcagcg cgtccgcgtg
     2761 ctggtcgacg tcacctatca gccggtgccg ctggagccgc ggctgtcgcc gctgaccggg
     2821 cgcgatctcg acatgtcggg ctgcttcctg cgctcgatgt cgccgtgcca tctgcagttc
     2881 ctgaaggaca tgggcgtgcg cgccaccctg gcggtgtcgc tggtggtcgg cggcaagctg
     2941 tggggcctgg ttgtctgtca ccattatctg ccgcgcttca tccgtttcga gctgcgggcg
     3001 atctgcaaac ggctcgccga aaggatcgcg acgcggatca ccgcgcttga gagc
//
