LOCUS       sequence-316227-        9378 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9378)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018271
FEATURES             Location/Qualifiers
     source          1..9378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2082..2102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2103..2126
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2152..2208
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2209..2226)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2209..2225)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2218..2240)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2434..2453)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2553..2575
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2613..2631)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3417..4616
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4847..5995)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6267..6638)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6671..6780
                     /note="incP origin of transfer"
     rep_origin      6933..7564
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8027..8615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8516..8535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8769..8786
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8903..8924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8939..8944,8945..8962,8963..8969)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8977..8993
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8982..9004
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9001..9017
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9001..9017
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgacgc
      361 ggaagcagtg gttcctttcc ggcttcgtcg ccttcacctg cctggcctcc actgcggccg
      421 gcgccgggcc gatcgagaac tatagccccg tgaccgcgca gcgtctcgaa aatcccgagc
      481 cctcgaactg gatgctgtac cgccgcacct atgatggcca gggctattcg ccgctcgatc
      541 agatcaacac ctcgaacgtc aagggcctca cccccgtgtg gaccttcgcc acgggcgtcg
      601 tcgagggcca tgaggccccg ccaatcgtca acaacggggt gatgttcgtt gcgacgccca
      661 tgggacaggt catcgccctg aacgccaaga ccggcgacga gtactggcgc tacaagcgac
      721 agctccccga tgacctcttc caactgcacc cgacctcgcg gggcgtgggc ctctgggagg
      781 acaagctgta cctggccacc accgatgatc acgtggttgc cctcgatgcc aagaccggca
      841 aggtggtttg ggacacgaag gtccaggact acaagaaggg ccagtacatg accctcatgc
      901 cgctgatcgt cgacggtaag gtgatcgtcg gtggctcggg cggcgagttc ggcgtgcgtg
      961 gatacgtcgc ggcgtacgat gccaaggacg gcaaggagct ctggcgcacc tacaccatcc
     1021 ccggcgaggg tgagccgggc cacgatacct ggcagggcga cgactggaag aacggcggcg
     1081 gctcggcctg gatgaccggc aactatgaca aggacaccaa gaccatctac tggggcgtcg
     1141 gcaacgcggc gccgtggccc ggcgaaaccc acccgggcga caatctctat accagctccg
     1201 tcctggcgct tgaccccaac aacgggaaga tcaaaaccta tcatcagtac catcaaaacg
     1261 acagctggga ctgggatgag gtcgaggccc ccatgctcat cgaccttcag cgtgatggcc
     1321 gcagcatcaa gtccctggtc caccctggcc gcgacgccat cttctgggtc ctggagcgca
     1381 ccccgacgaa gatcaattac gtcgcaggct ggcccttcgt gtccacggac gtctggaagg
     1441 gcatagacgc ggaaacgggc aagccgatcg tcgacccggc ccataagccc gtcatcggca
     1501 agcgcgtcga gttttgcccc tctttgtggg gcgggaagga ctggccgagc gccgcctaca
     1561 gccagaagac cgggctcgtc tatgtcccgg cgaacgagaa cttctgcggc ggcttcaccg
     1621 gcgaaaaggt ggccctgaag ccgggcgaac tgtggctggg gaccaagccg gaagacatcg
     1681 gactgaagac caagccggga gccgaccact tcggcgaact ccaggcgtgg gaccctgtca
     1741 ccggcaagaa agtgtggcag cataacttcc cgaaatcgca gcttttcggc agcgtgaccg
     1801 ccacagcggg cgatctgatc ttcgccgggg gcacgaacga ccgaaacttc cgggccttta
     1861 acgccaagac aggcgaactg ctctgggagc agaagaccaa cagcggcatc atgggcatgc
     1921 cggtgtccta tgagatcgat ggtactcagt acatcgccat ccagtccggc tggggcgtcg
     1981 atgcccaacg catccaggat gccctggtca ctaacaacat cggcatagag gccaacgtcc
     2041 ctcagggcgg tgtgatctgg gtcttcgccc tgaagaaggc cgaaaacctc tattttcagg
     2101 gacatcatca ccatcaccat caccactgac agctatgacc atgattacgc caagcttgca
     2161 tgcctgcagg tcgactctag aggatccccg ggtaccgagc tcgaattcac tggccgtcgt
     2221 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     2281 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     2341 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     2401 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     2461 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtatgctccc
     2521 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     2581 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     2641 taatgtcatg ataataatgg tttcttagca ccctttctcg gtccttcaac gttcctgaca
     2701 acgagcctcc ttttcgccaa tccatcgaca atcaccgcga gtccctgctc gaacgctgcg
     2761 tccggaccgg cttcgtcgaa ggcgtctatc gcggcccgca acagcggcga gagcggagcc
     2821 tgttcaacgg tgccgccgcg ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg
     2881 gccccaacag tgaagtagct gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa
     2941 cccgcctcgc agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt
     3001 cgcgtgccgg catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg
     3061 cgggcattcc cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta
     3121 tgattctccg ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag
     3181 tgccagtaaa gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga
     3241 ccgtctacgc cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc
     3301 aactttgtca tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag
     3361 tgataaagaa tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga
     3421 aacccaacat acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg
     3481 gcctgattat gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca
     3541 ccgcccacta tggcattctg ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg
     3601 tgctgggcgc gctgtcggat cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg
     3661 gcgccactgt cgactacgcc atcatggcga cagcgccttt cctttgggtt ctctatatcg
     3721 ggcggatcgt ggccggcatc accggggcga ctggggcggt agccggcgct tatattgccg
     3781 atatcactga tggcgatgag cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt
     3841 tcgggatggt cgcgggacct gtgctcggtg ggctgatggg cggtttctcc ccccacgctc
     3901 cgttcttcgc cgcggcagcc ttgaacggcc tcaatttcct gacgggctgt ttccttttgc
     3961 cggagtcgca caaaggcgaa cgccggccgt tacgccggga ggctctcaac ccgctcgctt
     4021 cgttccggtg ggcccggggc atgaccgtcg tcgccgccct gatggcggtc ttcttcatca
     4081 tgcaacttgt cggacaggtg ccggccgcgc tttgggtcat tttcggcgag gatcgctttc
     4141 actgggacgc gaccacgatc ggcatttcgc ttgccgcatt tggcattctg cattcactcg
     4201 cccaggcaat gatcaccggc cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc
     4261 tcggaatgat tgccgacggc acaggctaca tcctgcttgc cttcgcgaca cggggatgga
     4321 tggcgttccc gatcatggtc ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag
     4381 caatgttgtc caggcaggtg gatgaggaac gtcaggggca gctgcaaggc tcactggcgg
     4441 cgctcaccag cctgacctcg atcgtcggac ccctcctctt cacggcgatc tatgcggctt
     4501 ctataacaac gtggaacggg tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc
     4561 tgccggcgct gcgtcgcggg ctttggagcg gcgcagggca acgagccgat cgctgatcgt
     4621 ggaaacgata ggcctatgcc atgcgggtca aggcgacttc cggcaagcta tacgcgccct
     4681 agaattgtca attttaatcc tctgtttatc ggcagttcgt agagcgcgcc gtgcgtcccg
     4741 agcgatactg agcgaagcaa gtgcgtcgag cagtgcccgc ttgttcctga aatgccagta
     4801 aagcgctggc tgctgaaccc ccagccggaa ctgaccccac aaggccctag cgtttgcaat
     4861 gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc
     4921 aggcttcgcc gacctgctcg cgccacttct tcacgcgggt ggaatccgat ccgcacatga
     4981 ggcggaaggt ttccagcttg agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca
     5041 tccgtcgggc cgtcggcgac agcttgcggt acttctccca tatgaatttc gtgtagtggt
     5101 cgccagcaaa cagcacgacg atttcctcgt cgatcaggac ctggcaacgg gacgttttct
     5161 tgccacggtc caggacgcgg aagcggtgca gcagcgacac cgattccagg tgcccaacgc
     5221 ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga caggcattcc tcggccttcg
     5281 tgtaataccg gccattgatc gaccagccca ggtcctggca aagctcgtag aacgtgaagg
     5341 tgatcggctc gccgataggg gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt
     5401 cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt
     5461 ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca
     5521 gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat
     5581 cgaacaagga aagctgcatt tccttgatct gctgcttcgt gtgtttcagc aacgcggcct
     5641 gcttggcctc gctgacctgt tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg
     5701 tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc tcctgttcga
     5761 gacgacgcga acgctccacg gcggccgatg gcgcgggcag ggcaggggga gccagttgca
     5821 cgctgtcgcg ctcgatcttg gccgtagctt gctggaccat cgagccgacg gactggaagg
     5881 tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa
     5941 accccgcgtc gatcagttct tgcctgtatg ccttccggtc aaacgtccga ttcattcacc
     6001 ctccttgcgg gattgccccg actcacgccg gggcaatgtg cccttattcc tgatttgacc
     6061 cgcctggtgc cttggtgtcc agataatcca ccttatcggc aatgaagtcg gtcccgtaga
     6121 ccgtctggcc gtccttctcg tacttggtat tccgaatctt gccctgcacg aataccagct
     6181 ccgcgaagtc gctcttcttg atggagcgca tggggacgtg cttggcaatc acgcgcaccc
     6241 cccggccgtt ttagcggcta aaaaagtcat ggctctgccc tcgggcggac cacgcccatc
     6301 atgaccttgc caagctcgtc ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg
     6361 gcatcaccga accgcgccgt gcgcgggtcg tcggtgagcc agagtttcag caggccgccc
     6421 aggcggccca ggtcgccatt gatgcgggcc agctcgcgga cgtgctcata gtccacgacg
     6481 cccgtgattt tgtagccctg gccgacggcc agcaggtagg cctacaggct catgccggcc
     6541 gccgccgcct tttcctcaat cgctcttcgt tcgtctggaa ggcagtacac cttgataggt
     6601 gggctgccct tcctggttgg cttggtttca tcagccatcc gcttgccctc atctgttacg
     6661 ccggcggtag ccggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa
     6721 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc
     6781 ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta
     6841 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt
     6901 tatgcagcgg aaaagatccg tcgacccttt ccgacgctca ccgggctggt tgccctcgcc
     6961 gctgggctgg cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt
     7021 gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg aaaacttggc cctcactgac
     7081 agatgagggg cggacgttga cacttgaggg gccgactcac ccggcgcggc gttgacagat
     7141 gaggggcagg ctcgatttcg gccggcgacg tggagctggc cagcctcgca aatcggcgaa
     7201 aacgcctgat tttacgcgag tttcccacag atgatgtgga caagcctggg gataagtgcc
     7261 ctgcggtatt gacacttgag gggcgcgact actgacagat gaggggcgcg atccttgaca
     7321 cttgaggggc agagtgctga cagatgaggg gcgcacctat tgacatttga ggggctgtcc
     7381 acaggcagaa aatccagcat ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct
     7441 gtcttttaac ctgcttttaa accaatattt ataaaccttg tttttaacca gggctgcgcc
     7501 ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggcc
     7561 cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc tcggccgcga acggcctcac
     7621 cccaaaaatg gcagccaagc tgaccacttc tgcgctcggc ccttccggct ggctggttta
     7681 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7741 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7801 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7861 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7921 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7981 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8041 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8101 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8161 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8221 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8281 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8341 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8401 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8461 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8521 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8581 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8641 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8701 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8761 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8821 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     8881 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     8941 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     9001 caggaaacag ctatgaccat gattacgcca agctagcttc ccgcttggtc gggccgcttc
     9061 gcgagggccc gttgacgaca acggtgcgat gggtcccggc cccggtcaag acgatgccaa
     9121 tacgttgcga cactacgcct tggcactttt agaattgcct tatcgtcctg ataagaaatg
     9181 tccgaccagc taaagacatc gcgtccaatc aaagcctaga aaatataggc gaagggacgc
     9241 taataagtct ttcataagac cgcgcaaatc taaaaatatc cttagattca cgatgcggca
     9301 cttcggatga cttccgagcg agcctggaac ctcagaaaaa cgtctgagag ataccgcgga
     9361 tctcacacag gaaatttc
//
