LOCUS       sequence-316581-        9483 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018343
FEATURES             Location/Qualifiers
     source          1..9483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2187..2207
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2208..2231
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2257..2313
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2314..2331)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2314..2330)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2323..2345)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2539..2558)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2658..2680
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2718..2736)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3522..4721
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4952..6100)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6372..6743)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6776..6885
                     /note="incP origin of transfer"
     rep_origin      7038..7669
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8132..8720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8621..8640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8874..8891
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9008..9029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9044..9049,9050..9067,9068..9074)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9082..9098
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9087..9109
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9106..9122
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9106..9122
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgcggg
      361 tctaccatgc gctcgtcctc gggctggtcc ttgccgggtg caagaaggtg ggcccgtcct
      421 ctgacagcac ggcggcgcgc gtgcagggtg gcgcgccgat cgtggccgac gcgcgcatcg
      481 gcacgcctaa ccccccgggc ggcgaagggg agtgggtcat gcccgcgcgc gactacgcga
      541 gcagccgcta ttccaccctc gcgcagatca cgtcggccaa tgtcaagaac ctcaaggccg
      601 cttggacgtt ctccacaggc gtgctccgcg gccatgaggg acagccgctg gtggtcaaca
      661 aaaccatgta tctcgtgacc ccgtacccca acgtcgccta tgccatcgac ctcacgcagc
      721 ccggctaccc gctgaagtgg aagttccgcc ccgagaacgc acaggccgcc gccggtacgg
      781 cctgctgcga cgtggtcaac cggggcgccg cgtacgccga tggaaagatc gtgtataacc
      841 tcctggatgg ccacacggtc gcggtggacg ccgagtccgg gaagcagctg tggcgcgtcc
      901 agatggccga cctcaaccgc ggggagacga tcacgatggc gcccatcgtc gtcaagaacc
      961 gggtgatcgt cggcagctcg ggaggggaga tgggcgtgcg agggtggatc gcggcgctcg
     1021 atctggcgac gggcagggag ctgtggcggg cgtacaacac cggctcggac gcggacgtca
     1081 agatcggcgc gcggttcaag cccttctatg ccaccgagcg cggcaaggac ctcggcctta
     1141 cgtcctggcc gggcgactcg tggaaggtcg gcggcggcgc cgtctggggc tggctgagct
     1201 acgacccgac cctgaacctg gtctaccatg gcacctcgaa cccgggcccc tggaaccagg
     1261 acgtccggcc gggcgacaac aagtggacct gcgcgatctt ggcgcgggac gccgacaccg
     1321 gcgagctggt ctgggccttc caggtcacgc ctcacgacct gtgggattac gacgcagtga
     1381 acgagaacat cctcgtggac atgccggtca agggacagcc ccgcaaggcc ctcgtgcact
     1441 ttgatcgcaa cgggttcgcg tacacccttg accgccagac gggcgaggtc ctgctggccc
     1501 agccgtacgt ccccatgaac tgggccaagt acattaacct gcagaccggc cgacccgaga
     1561 tcgactcgtc caaggccacc aagcagggga agctcgtcaa gaacatctgc ccgtcgctgg
     1621 agggcggaaa gaaccaacag cccgcggcct tctcgcccca gaccggcctg ttctacgtcc
     1681 cgacgaacaa tctgtgcatg gacttcgagt cccggccagt cagctacatt gccgggaccc
     1741 cgtacatcgg cgcgaacgcc ccggagaccg gcggaccggg tgactataag ggcgagttca
     1801 tggcctggga cgccgccaat ggccggaagg tgtggggcat ccgggagccc ttccccgtct
     1861 ggggcggggc gctggtcacc gccggcaacg tggtcttcta tggcacgctg gatggctggt
     1921 ttaaggcggc caacgcccgt accggcgagg tgctgtggca gttcaaggtc gggtcgggcg
     1981 tggtcggcaa cccgatcacg tacaccgccc ccgacggcaa gcagtatatc gcggtctatt
     2041 cgggcatcgg gggcgatatg gggctcctga tcgcaggcga cgtggcggcg aacctcccgt
     2101 tcgatgtgcg cgagcgtggt accaccctcc ccgacctggc caagtacacc tcctggggcg
     2161 gcatgctgtt cgtcttcgcg ctcgccgaaa acctctattt tcagggacat catcaccatc
     2221 accatcacca ctgacagcta tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac
     2281 tctagaggat ccccgggtac cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga
     2341 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     2401 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     2461 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     2521 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     2581 cccgccaaca cccgctgacg cgccctgacg ggcttgtatg ctcccggcat ccgcttacag
     2641 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     2701 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     2761 aatggtttct tagcaccctt tctcggtcct tcaacgttcc tgacaacgag cctccttttc
     2821 gccaatccat cgacaatcac cgcgagtccc tgctcgaacg ctgcgtccgg accggcttcg
     2881 tcgaaggcgt ctatcgcggc ccgcaacagc ggcgagagcg gagcctgttc aacggtgccg
     2941 ccgcgctcgc cggcatcgct gtcgccggcc tgctcctcaa gcacggcccc aacagtgaag
     3001 tagctgattg tcatcagcgc attgacggcg tccccggccg aaaaacccgc ctcgcagagg
     3061 aagcgaagct gcgcgtcggc cgtttccatc tgcggtgcgc ccggtcgcgt gccggcatgg
     3121 atgcgcgcgc catcgcggta ggcgagcagc gcctgcctga agctgcgggc attcccgatc
     3181 agaaatgagc gccagtcgtc gtcggctctc ggcaccgaat gcgtatgatt ctccgccagc
     3241 atggcttcgg ccagtgcgtc gagcagcgcc cgcttgttcc tgaagtgcca gtaaagcgcc
     3301 ggctgctgaa cccccaaccg ttccgccagt ttgcgtgtcg tcagaccgtc tacgccgacc
     3361 tcgttcaaca ggtccagggc ggcacggatc actgtattcg gctgcaactt tgtcatgctt
     3421 gacactttat cactgataaa cataatatgt ccaccaactt atcagtgata aagaatccgc
     3481 gcgttcaatc ggaccagcgg aggctggtcc ggaggccaga cgtgaaaccc aacatacccc
     3541 tgatcgtaat tctgagcact gtcgcgctcg acgctgtcgg catcggcctg attatgccgg
     3601 tgctgccggg cctcctgcgc gatctggttc actcgaacga cgtcaccgcc cactatggca
     3661 ttctgctggc gctgtatgcg ttggtgcaat ttgcctgcgc acctgtgctg ggcgcgctgt
     3721 cggatcgttt cgggcggcgg ccaatcttgc tcgtctcgct ggccggcgcc actgtcgact
     3781 acgccatcat ggcgacagcg cctttccttt gggttctcta tatcgggcgg atcgtggccg
     3841 gcatcaccgg ggcgactggg gcggtagccg gcgcttatat tgccgatatc actgatggcg
     3901 atgagcgcgc gcggcacttc ggcttcatga gcgcctgttt cgggttcggg atggtcgcgg
     3961 gacctgtgct cggtgggctg atgggcggtt tctcccccca cgctccgttc ttcgccgcgg
     4021 cagccttgaa cggcctcaat ttcctgacgg gctgtttcct tttgccggag tcgcacaaag
     4081 gcgaacgccg gccgttacgc cgggaggctc tcaacccgct cgcttcgttc cggtgggccc
     4141 ggggcatgac cgtcgtcgcc gccctgatgg cggtcttctt catcatgcaa cttgtcggac
     4201 aggtgccggc cgcgctttgg gtcattttcg gcgaggatcg ctttcactgg gacgcgacca
     4261 cgatcggcat ttcgcttgcc gcatttggca ttctgcattc actcgcccag gcaatgatca
     4321 ccggccctgt agccgcccgg ctcggcgaaa ggcgggcact catgctcgga atgattgccg
     4381 acggcacagg ctacatcctg cttgccttcg cgacacgggg atggatggcg ttcccgatca
     4441 tggtcctgct tgcttcgggt ggcatcggaa tgccggcgct gcaagcaatg ttgtccaggc
     4501 aggtggatga ggaacgtcag gggcagctgc aaggctcact ggcggcgctc accagcctga
     4561 cctcgatcgt cggacccctc ctcttcacgg cgatctatgc ggcttctata acaacgtgga
     4621 acgggtgggc atggattgca ggcgctgccc tctacttgct ctgcctgccg gcgctgcgtc
     4681 gcgggctttg gagcggcgca gggcaacgag ccgatcgctg atcgtggaaa cgataggcct
     4741 atgccatgcg ggtcaaggcg acttccggca agctatacgc gccctagaat tgtcaatttt
     4801 aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga
     4861 agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg
     4921 aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc aggtcatcat
     4981 tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct
     5041 gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg aaggtttcca
     5101 gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg
     5161 gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca gcaaacagca
     5221 cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca cggtccagga
     5281 cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc
     5341 ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa taccggccat
     5401 tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga
     5461 taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca tcgtcggccc
     5521 gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt
     5581 tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca gagcgggccg
     5641 tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac aaggaaagct
     5701 gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga
     5761 cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg
     5821 tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct
     5881 ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg tcgcgctcga
     5941 tcttggccgt agcttgctgg accatcgagc cgacggactg gaaggtttcg cggggcgcac
     6001 gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc gcgtcgatca
     6061 gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct tgcgggattg
     6121 ccccgactca cgccggggca atgtgccctt attcctgatt tgacccgcct ggtgccttgg
     6181 tgtccagata atccacctta tcggcaatga agtcggtccc gtagaccgtc tggccgtcct
     6241 tctcgtactt ggtattccga atcttgccct gcacgaatac cagctccgcg aagtcgctct
     6301 tcttgatgga gcgcatgggg acgtgcttgg caatcacgcg caccccccgg ccgttttagc
     6361 ggctaaaaaa gtcatggctc tgccctcggg cggaccacgc ccatcatgac cttgccaagc
     6421 tcgtcctgct tctcttcgat cttcgccagc agggcgagga tcgtggcatc accgaaccgc
     6481 gccgtgcgcg ggtcgtcggt gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg
     6541 ccattgatgc gggccagctc gcggacgtgc tcatagtcca cgacgcccgt gattttgtag
     6601 ccctggccga cggccagcag gtaggcctac aggctcatgc cggccgccgc cgccttttcc
     6661 tcaatcgctc ttcgttcgtc tggaaggcag tacaccttga taggtgggct gcccttcctg
     6721 gttggcttgg tttcatcagc catccgcttg ccctcatctg ttacgccggc ggtagccggc
     6781 cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag
     6841 aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg
     6901 atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag
     6961 gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaag
     7021 atccgtcgac cctttccgac gctcaccggg ctggttgccc tcgccgctgg gctggcggcc
     7081 gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc
     7141 cgccggcgtt gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac
     7201 gttgacactt gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga
     7261 tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac
     7321 gcgagtttcc cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac
     7381 ttgaggggcg cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt
     7441 gctgacagat gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc
     7501 agcatttgca agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct
     7561 tttaaaccaa tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg
     7621 cgcacgccga aggggggtgc ccccccttct cgaaccctcc cggcccgcta acgcgggcct
     7681 cccatccccc caggggctgc gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc
     7741 caagctgacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7801 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7861 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7921 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7981 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8041 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8101 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8161 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8221 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     8281 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8341 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8401 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8461 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8521 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8581 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8641 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8701 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8761 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8821 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8881 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     8941 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     9001 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     9061 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     9121 accatgatta cgccaagcta gcttcccgct tggtcgggcc gcttcgcgag ggcccgttga
     9181 cgacaacggt gcgatgggtc ccggccccgg tcaagacgat gccaatacgt tgcgacacta
     9241 cgccttggca cttttagaat tgccttatcg tcctgataag aaatgtccga ccagctaaag
     9301 acatcgcgtc caatcaaagc ctagaaaata taggcgaagg gacgctaata agtctttcat
     9361 aagaccgcgc aaatctaaaa atatccttag attcacgatg cggcacttcg gatgacttcc
     9421 gagcgagcct ggaacctcag aaaaacgtct gagagatacc gcggatctca cacaggaaat
     9481 ttc
//
