LOCUS       sequence-316799-        2799 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Untagged Amp resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 1 pCOLI_A_NoTag
FEATURES             Location/Qualifiers
     source          1..2799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             22..44
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(170..188)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      184..231
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        296..387
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1252..1291
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1322..1910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1811..1830
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2064..2081
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2096..2235
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2161..2180)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2321..2343
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2337..2528)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2765..2784
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2765..2783
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccctccggct ccggaaataa ttttgtttaa ctttaagaag gagactggat ccgaatctag
       61 agcctgcagt ctcgacaagc ttgtcgagaa gtactagagg atgtacaacg cgtgcgcgct
      121 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      181 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
      241 ggaactatat cgggattagc ccgcctaatg agcgggcttt tttttaattc ccctatttgt
      301 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      361 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      421 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct cgtgaaagta
      481 aaagacgcag aggaccaatt gggggcacga gtgggataca tagaactgga cttgaatagc
      541 ggtaaaatcc ttgagagttt tcgccctgaa gagcgttttc caatgatgag cactttcaaa
      601 gttctgctat gtggagcagt attatcccgt gtagatgcgg ggcaagagca actcggacga
      661 cgaatacact attcgcagaa tgacttggtt gaatactccc cagtgacaga aaagcacctt
      721 acggacggaa tgacggtaag agaattatgt agtgccgcca taacgatgag tgataacact
      781 gcggcgaact tacttctgac aaccatcggt ggaccgaagg aattaaccgc ttttttgcac
      841 aatatgggag accatgtaac tcgccttgac cgttgggaac cagaactgaa tgaagccata
      901 ccaaacgacg agcgagacac cacaatgcct gcggcaatgg caacaacatt acgcaaacta
      961 ttaactggcg aactacttac tctggcttca cggcaacaat taatagactg gcttgaagcg
     1021 gataaagttg caggaccact actgcgttcg gcacttcctg ctggctggtt tattgctgat
     1081 aaatctgggg caggagagcg tggttcacgg ggtatcattg ccgcacttgg accagatggt
     1141 aagccttccc gtatcgtagt tatctacacg acgggtagtc aggcaactat ggacgaacga
     1201 aatagacaga ttgctgaaat aggggcttca ctgattaagc attggtaaac cgatacaatt
     1261 aaaggctcct tttggagcct ttttttttgg acggaccggt agaaaagatc aaaggatctt
     1321 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1381 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1441 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     1501 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1561 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1621 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1681 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1741 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1801 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1861 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1921 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1981 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2041 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     2101 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag
     2161 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     2221 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     2281 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     2341 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg
     2401 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc
     2461 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
     2521 ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg ataccgatga
     2581 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     2641 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     2701 gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat cgatcccgcg
     2761 aaattaatac gactcactat agggagacca caacggttt
//
