LOCUS       sequence-31760-e         574 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Huang HH, Camsund D, Lindblad P, Heidorn T
  TITLE     Design and characterization of molecular tools for a Synthetic 
            Biology approach towards developing cyanobacterial biotechnology.
  JOURNAL   Nucleic Acids Res. 2010 May . 38(8):2577-93.
  PUBMED    20236988
REFERENCE   2  (bases 1 to 574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2010 May . 38(8):2577-93."
COMMENT     Sequence Label: pPMQAK1
FEATURES             Location/Qualifiers
     source          1..574
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(192..296)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     364..382
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(386..429)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    432..453
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
       61 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
      181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
      241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
      301 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
      361 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
      421 gatgatttct ggaattcgcg gccgcttcta gagactggct gtgtataagg gagcctgaca
      481 tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga
      541 gatcagccac ttcttccccg ataacggaga ccgg
//
