LOCUS       sequence-31791-e         488 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Entry vector with TRE promoter driving mouse G beta 2 miR30-based 
            shRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pEN_TmiR_Gb2-K
FEATURES             Location/Qualifiers
     source          1..488
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           70..191
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           219..233
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           261..387
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(420..444)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gaagacaccg ggaccgatcc agcctccgcg gccccgaatc tagtcgacta gggataacag
       61 ggtaattgtt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac atcttggaaa
      121 cacttgctgg gattacttct tcaggttaac ccaacagaag gctcgagaag gtatattgct
      181 gttgacagtg agcgcgctca tgtattccca cgacaatagt gaagccacag atgtattgtc
      241 gtgggaatac atgagcatgc ctactgcctc ggaattcaag gggctacttt aggagcaatt
      301 atcttgttta ctaaaactga ataccttgct atctctttga tacattttta caaagctgaa
      361 ttaaaatggt ataaattaaa tcactttttt caattcgcgg ccgcactcga gatatctaga
      421 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
      481 cgaacagg
//
