LOCUS       sequence-318508-       13248 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express mouse p150 protein with PIP2 deletion mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13248)
  AUTHORS   Cheng L, Zhang X, Wang Y, Gan H, Xu X, Lv X, Hua X, Que J, Ordog T, 
            Zhang Z
  TITLE     Chromatin Assembly Factor 1 (CAF-1) facilitates the establishment of
            facultative heterochromatin during pluripotency exit.
  JOURNAL   Nucleic Acids Res. 2019 Dec 2;47(21):11114-11131. doi: 
            10.1093/nar/gkz858.
  PUBMED    31586391
REFERENCE   2  (bases 1 to 13248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-12-2- 2"; volume: "47"; issue: 
            "21"; pages: "11114-11131"
COMMENT     Sequence Label: pWPXL-p150-PIP2-Delta
FEATURES             Location/Qualifiers
     source          1..13248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2145..3323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2376..3314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3271..3291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3371..3488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    5309..5320
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     regulatory      6275..6284
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6281..6283,6284..6286,6287..6997)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6281..6283,6284..6286,6287..6997)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6326..6347)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6587..6606)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6934..6955
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7079..7667
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7132..7152)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7550..7561)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7811..7844)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7864..8044
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8069..8087)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8070..8087)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8377..8396)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8610..8629)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8729..8751
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8789..8807)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8875..8979
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8980..9048,9049..9840)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9198..9217)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10011..10599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10500..10519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10753..10770
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10840..10860)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10845..11174
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11025..11160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12308..12373
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12503..12523
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12948..13082
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12985..13004)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13039..13058
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     2101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     2701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     2761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     2821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     2881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     2941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3301 ttcttccatt tcaggtgtcg tgaggaattt cgacatttaa atttaattaa tctcgacggt
     3361 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3421 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3481 aaaattttat cgatcacgag actagcctcg aggtttaaac tacgggatcc cgatgttgga
     3541 ggagccggag gccgcgacgc ggacagccgc ggccgtggat tgcaaagaca gacccgggtt
     3601 cccagtgaag aggttaatac aagcccgctt gcccttcaaa cgcctgaacc ttgttcccaa
     3661 ggagaaagtt gaggaagaca catcaccaaa agcagctgtg gaaagcaaag tcccggatct
     3721 acaattgtct ttgggcactt ttgaaagcca gtgtcacaca ggctctcacg taggcttgag
     3781 tacaaagttg gtcggtggcc agggccccat tgatagcttc ttgagggcaa caatcaagcc
     3841 agtaccgagt gtggtcatta tcgacctgac ggagaactgc agtgacattc cagacagccc
     3901 tgagggccac agtgaactaa gtcctgacac tgctggtgtg gtcaccacag tggaaggagc
     3961 tgccaagcag caggagcact ctgcagcaga gctgtgtctc ctagagaccc cttcagacat
     4021 cacttgtcac atggaagagg agcctgggag tccaggagac ccaaagagga cgggtgactg
     4081 tcaggctggt tcactacaga gctgtccgga gctgacccca ggttctagga catgccccac
     4141 caaggagtta tcaagctgga gcaaggcagg ggatctccta tttatagaga aagtgcctgt
     4201 ggtggtgcta gaggacatcc tggccactaa gccctccatt gcctctcttc ccatgatgtc
     4261 cctggatagg agtgtcactt cagagagtga aatactggag tcctgccccg aggatgactc
     4321 catactgagc cactcctcca ccaactcctc ttctccaacc agctctcctg agggaccatc
     4381 cacaccccca gaacatcggg gtgggagaag ctccccctcc accccagcct gcagagttgc
     4441 caagaacttt gtcaaaggct ccacagagaa gggcaggagc aaactgcaca gagatagaga
     4501 acagcagagg gaagagaagg aaaagctgag ggaagagata cgacgagcca aggaggaggc
     4561 acggaagaag aaggaggagg agaaggagct gaaggaaaag gagcggaggg aaaagcggga
     4621 gaaagatgag aaggagaagg cggagaagca gcggctaaag gaggaaaaac gcaaggagcg
     4681 gcaggaagct ctggaggcta aactggagga gaagaggaag aaggaggaag agaagcggtt
     4741 acgggaggaa gagaagcggt tacgggaaga agagaagcgc attaaggcag aaaaggcaga
     4801 gcagaaaccg aagactccgc aggctcccaa gaccctggcc ggttcctgtg gtaagtttgc
     4861 cccctttgaa atcaaagaac acatggtgct ggctccacgg tgccgagctg ccttggatca
     4921 agacctgtgc gatcagctgg accagctcct gcagcagcag agtgtggcaa gcaccttcct
     4981 cagtgaccta aaaagccggc tgcccttgag atcggggccc acccgggtct gtggccatga
     5041 cacagacatc atgaacaggg atgtggtcat tgtggagagc agcaaggtgg acggtgtgtc
     5101 tgaaaggaag aagtttggcc gcatgaagct tctgcagttc tcagagaacc accggccagc
     5161 atactggggg acgtggaaca agaagacagc catcatccgc ccccggaacc cctgggccca
     5221 ggacaaagac cttcttgact atgaggtaga cagtgacgat gagtgggaag aggaggaacc
     5281 tggggagtct ttgtcccaca gtgaggggga tgaggatgac gatgtggggg aagatgagga
     5341 tgaggacgat ggcttctttg tgccccatgg gtacttatct gaagatgaag gcgtgactga
     5401 ggagtgtgca gaccctgaga accacaaagt acaccagaag ctgaaggcca aggagtggga
     5461 tgagctccta gccaagggca agcgctttcg tgtgctgcag cctgtgcacg tgggatgtgt
     5521 atgggcatca gaggcagcca actgcacgag ttccgacttg aagctgctgc agcagttcac
     5581 tgcatgcctg ctggatgtgg catcccctga tgagccagaa cccggggcct ccaggaggga
     5641 aaagagggac cagcatatcc tggcccagtt gcttccgctg ctacatggca atgtgaatgg
     5701 gagcaaggtg atcatccatg aattccagga gcaatgccgc aggggcctgc tgacccttcc
     5761 aagtcccacc ccacacctgc agatgcccaa cttggaggat gctgttgctg tgccatccaa
     5821 ggccaggctg aagcgcctta tctctgagaa ctctgcctat gagaagcggc ccaacttccg
     5881 tatgtgctgg tatgtgcacc cagaggtgct caagagcttt ggccaggagt gcctgccagt
     5941 gccctgccag tggacctaca tcaccaccat gccctcagca cccagggaag acagtggcag
     6001 tgcttctact gaggggcctg gccagagcac acctatgcta ctcaagcgga agccagcggc
     6061 caccatgtgc atcacgcagt ttatgaagaa gcggcgctat gatggacagg ttgggtctgg
     6121 ggacatggat ggcttccagg cagacacaga agaggatgaa gaggatgaca cagactgtat
     6181 gatcatagat gtgccagatg ttgggagtga cgtgtcagaa gctcccatac ctgctcccac
     6241 actttgcaag gcacgcgtcc tagcgctacc ggtcgccacc atggtgagca agggcgagga
     6301 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     6361 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     6421 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     6481 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     6541 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     6601 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     6661 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     6721 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     6781 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     6841 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     6901 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     6961 cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat ctcgactagc
     7021 tagtagctag ctagctagtc gagctcaagc ttcgaattcc cgggactagt catatgataa
     7081 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     7141 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     7201 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     7261 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     7321 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7381 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     7441 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     7501 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7561 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7621 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc ggtacgtatg
     7681 gccaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7741 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat gggatcaatt
     7801 caccatggga ataacttcgt atagcataca ttatacgaag ttatgctgct ttttgcttgt
     7861 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7921 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7981 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8041 agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga
     8101 tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt
     8161 gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag
     8221 tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg
     8281 tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg
     8341 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     8401 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     8461 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     8521 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     8581 agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     8641 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     8701 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     8761 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     8821 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     8881 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8941 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9001 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9061 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     9121 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     9181 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     9241 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     9301 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     9361 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     9421 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     9481 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     9541 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     9601 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     9661 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     9721 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9781 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9841 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9901 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9961 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10021 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10081 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10141 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10201 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10261 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    10321 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    10381 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    10441 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    10501 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    10561 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    10621 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    10681 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    10741 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    10801 cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt
    10861 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    10921 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    10981 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
    11041 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
    11101 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    11161 taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca
    11221 ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg
    11281 gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt
    11341 taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg
    11401 gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg
    11461 aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac
    11521 tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg
    11581 agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc
    11641 tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg
    11701 tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc
    11761 cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct
    11821 ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc
    11881 gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg
    11941 agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc
    12001 tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac
    12061 tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc
    12121 ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac
    12181 gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt
    12241 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
    12301 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
    12361 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt
    12421 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc
    12481 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt
    12541 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg
    12601 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
    12661 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct
    12721 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt
    12781 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag
    12841 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
    12901 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
    12961 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    13021 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    13081 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
    13141 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    13201 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtag
//
