LOCUS       sequence-318817-        9186 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Tet-responsive retroviral expression vector. Contains INPP5E. Also 
            contains SGFP tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Roman-Fernandez A, Roignot J, Sandilands E, Nacke M, Mansour MA, 
            McGarry L, Shanks E, Mostov KE, Bryant DM
  TITLE     The phospholipid PI(3,4)P2 is an apical identity determinant.
  JOURNAL   Nat Commun. 2018 Nov 28;9(1):5041. doi: 10.1038/s41467-018-07464-8.
  PUBMED    30487552
REFERENCE   2  (bases 1 to 9186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-11-28"; volume: "9(1):5041. doi"; pages: " 
            10.1038/s41467-018-07464-8"
COMMENT     Sequence Label: pRevTRE SGFP2 Inpp5E WT shRNA resistant
FEATURES             Location/Qualifiers
     source          1..9186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(90..108,109..131,132..150,151..173,174..192,193..215,
                     216..234,235..257,258..276,277..299,300..318,319..341,
                     342..360)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        393..431
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     428..452
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      566..575
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(572..574,575..577,578..1288)
                     /codon_start=1
                     /product="strongly enhanced green fluorescent protein 
                     (Kremers et al., 2007)"
                     /label=SGFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(878..897)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1225..1246
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             3331..3924
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4027..4049)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4135..4275
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4190..4209
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4290..4307)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4461..5049)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4541..4560)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5220..6011,6012..6080))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5843..5862
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6081..6185)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6253..6271
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             6843..7018
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    7081..7280
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             7481..7897
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7827..7849
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7865..7881
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             7953..8972
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             7953..8972
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
ORIGIN
        1 gcccacgcac tcgggtgatc tgactgatcc cgcagattgg agatcgccgc ccgtgcctgc
       61 cgattgggtg cagatctcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
      121 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
      181 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
      241 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
      301 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
      361 aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata
      421 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
      481 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaattc gagctcggta
      541 cccggggatc ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac
      601 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      661 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      721 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
      781 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      841 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      901 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      961 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1021 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     1081 caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1141 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
     1201 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1261 cactctcggc atggacgagc tgtacaagtc cggactcaga tctcgagctc aagcttcgaa
     1321 ttctgcagtc gacggtacca tgccgtccaa ggcggagaat ctgcggccct ccgagccggc
     1381 cccgcagccg ccggaaggga ggacgctcca aggacagctt cctggcgctc cgccggccca
     1441 gcgcgcgggg tccccacccg atgctccggg ctccgagagc cccgcgcttg cctgcagcac
     1501 tccggccacg cccagcggcg aggacccgcc agcccgagca gcacccatcg ccccgcggcc
     1561 ccccgccagg cctcgactgg agcgagccct gtccctggac gacaagggct ggaggaggag
     1621 gcgttttcga ggcagccagg aggacctgga agcccggaat gggaccagtc cctccagggg
     1681 ctcagtgcag agtgagggcc ccggggcccc cgcccacagc tgctccccgc cctgcctgag
     1741 cacctccttg caggaaatcc ccaagtcccg cggggtcctg agcagtgaga gagggagccc
     1801 gtcctcgggg ggtaaccctc tctctggggt ggccagcagc tccccgaacc tcccgcacag
     1861 agacgccgcc gtggcgggga gctcgcccag gctgcccagc ctgctgcccc cgcgcccacc
     1921 gcctgccctg agcctggaca tcgcctccga ctccctgagg acagcaaaca aggtcgactc
     1981 ggatcttgca gactacaagc tccgcgcgca gccgctcctg gtgcgggccc acagcagcct
     2041 gggccccggc cggccgcgga gccccctggc ctgcgacgac tgttcccttc gctcggccaa
     2101 atcctccttc agcctcctgg cgcccatccg cagcaaggac gtccgcagca ggagttacct
     2161 ggagggcagc ctcctggcca gcggggccct gttgggggcg gatgagctgg cccgctactt
     2221 cccagaccgg aacgtggcac tcttcgtggc cacctggaac atgcagggcc agaaggagct
     2281 cccgcccagc ctggacgagt tcctgctccc agccgaggcc gactatgccc aggacctgta
     2341 tgtcatcggg gtccaggagg gctgttctga caggcgggag tgggagactc gtctgcagga
     2401 gacgctgggc ccgcactatg tgctgctgtc ctcggcggcc cacggcgtgc tctacatgtc
     2461 gctcttcatc cgcagggacc tcatctggtt ctgctcagag gtggagtgct ccacggtgac
     2521 cacacgcatc gtgtctcaga tcaagaccaa gggggccttg ggcatcagct tcaccttttt
     2581 tggcacttcc ttcctcttca tcacgtccca cttcacctca ggtgacggga aggtggcgga
     2641 gcggctgctg gactacacca ggactgtaca agccctggtc ctgcccagaa atgtgcccga
     2701 caccaacccc tatcgctcca gcgcagcgga cgtcaccacc cgcttcgatg aggtgttctg
     2761 gtttggagac ttcaacttcc gcctgagtgg cgggcgcaca gtcgtggacg ccctcctgtg
     2821 ccagggcctg gtggtggacg tgccggcgct gctgcagcac gaccagctca tccgggagat
     2881 gcggaaggga tccatcttca agggcttcca ggagccggac atccacttcc tcccatcata
     2941 caagtttgac atcgggaagg acacgtacga cagcacctcc aagcagagga cgccctcata
     3001 cacggaccgc gtcttgtaca gaagccgcca caagggtgac atctgtcctg tgagctactc
     3061 ttcctgcccc gggatcaaga cgtccgacca ccgccctgtg tatggcctct tccgggtgaa
     3121 agtgaggccg gggcgagaca acattccgtt ggcagctggc aaatttgata gagaactgta
     3181 cttactagga attaaaagac ggatttcgaa ggagattcag aggcagcaag cactacagag
     3241 tcagaactct agcaccatct gctccgtttc ttgaagcttg ttaacatcga taaaataaaa
     3301 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     3361 gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa
     3421 gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt
     3481 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc
     3541 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
     3601 ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc
     3661 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
     3721 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg
     3781 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc
     3841 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
     3901 acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag
     3961 ggaccaccga cccaccaccg ggaggtaagc tggctgcctc gcgcgtttcg gtgatgacgg
     4021 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     4081 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     4141 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     4201 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     4261 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     4321 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     4381 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     4441 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     4501 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     4561 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     4621 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     4681 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     4741 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     4801 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4861 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     4921 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4981 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     5041 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     5101 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     5161 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     5221 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     5281 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     5341 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     5401 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     5461 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     5521 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     5581 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     5641 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     5701 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     5761 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     5821 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     5881 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     5941 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     6001 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     6061 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     6121 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     6181 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     6241 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt cataccagat
     6301 caccgaaaac tgtcctccaa atgtgtcccc ctcacactcc caaattcgcg ggcttctgcc
     6361 tcttagacca ctctacccta ttccccacac tcaccggagc caaagccgcg gcccttccgt
     6421 ttctttgctt ttgaaagacc ccacccgtag gtggcaagct agcttaagta acgccacttt
     6481 gcaaggcatg gaaaaataca taactgagaa tagaaaagtt cagatcaagg tcaggaacaa
     6541 agaaacagct gaataccaaa caggatatct gtggtaagcg gttcctgccc cggctcaggg
     6601 ccaagaacag atgagacagc tgagtgatgg gccaaacagg atatctgtgg taagcagttc
     6661 ctgccccggc tcggggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
     6721 tctagtgaat catcagatgt ttccagggtg ccccaaggac ctgaaaatga ccctgtacct
     6781 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttcc gctctccgag
     6841 ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcttccgata gactgcgtcg
     6901 cccgggtacc cgtattccca ataaagcctc ttgctgtttg catccgaatc gtggtctcgc
     6961 tgttccttgg gagggtctcc tctgagtgat tgactaccca cgacgggggt ctttcatttg
     7021 ggggctcgtc cgggatttgg agacccctgc ccagggacca ccgacccacc accgggaggt
     7081 aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatgt ttgatgttat
     7141 gcgcctgcgt ctgtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac
     7201 tgacgagttc tgaacacccg gccgcaaccc tgggagacgt cccagggact ttgggggccg
     7261 tttttgtggc ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt
     7321 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc
     7381 ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg
     7441 tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc
     7501 cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt
     7561 agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt
     7621 cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt
     7681 cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc
     7741 cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc
     7801 tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc
     7861 ctccctttat ccagccctca ctccttctct aggcgccgga attccgatct gatagcttgc
     7921 cacaacccgt accaaagatg gatagatccg gaaagcctga actcaccgcg acgtctgtcg
     7981 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     8041 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     8101 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     8161 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
     8221 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     8281 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     8341 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     8401 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     8461 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     8521 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     8581 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     8641 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     8701 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
     8761 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     8821 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     8881 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     8941 gccccagcac tcgtccgagg gcaaaggaat agagtagatg ccgaccgaac aagagctgat
     9001 ttcgagaacg cctcagccag caactcgcgc gagcctagca aggcaaatgc gagagaacgg
     9061 ccttacgctt ggtggcacag ttctcgtcca cagttcgcta agctcgctcg gctgggtcgc
     9121 gggagggccg gtcgcagtga ttcaggccct tctggattgt gttggtcccc agggcacgat
     9181 tgtcat
//
