LOCUS       sequence-319127-       10273 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Integration Vector, assembled with pHIE048 (TUPV1), pJM451 (TUPV2), 
            pPD612.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10273)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 10273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pHIE064 ZF43x6-S mKate2 EF1a EBFP2-P2A-BlastR
FEATURES             Location/Qualifiers
     source          1..10273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1031..1068
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1079..1678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1079..1098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1575..1595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1791..1810)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1856..1911
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1910..1929)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      3217..3226
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3223..3921
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3972..4196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5245..6427
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5476..6418
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6375..6395
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     6429..6453
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6528..6537
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6534..6536,6537..6539,6540..7250)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(6579..6600)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             7278..7334
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7347..7745
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7796..8020
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(8879..9688)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9602..9621
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9680..9699
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(join(9847..10273,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9927..9946)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      241 ttatcaaaaa ggatcttcac ctagatcctt tctcgagctt tcggagtgag atggagtggc
      301 cactgtggaa ctgggcctag catcgcgata agctctagag ggacagcccc cccccaaagc
      361 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      421 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      541 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
      601 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
      661 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
      961 gcgcgcctgg tggcgaacga tcaacagacg ggagatctac tattgggaca gcagagatcc
     1021 agttggtaat ggcttgtcga cgacggcggt ctccgtcgtc aggatcatag gtgccaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc
     1681 ttccactgga ttgtacaatt accaactgaa ttcactcctc aggtgcaggc tgcctatcag
     1741 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     1801 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     1861 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1921 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1981 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     2041 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     2101 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     2161 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     2221 cttctcttat ggagatccgg gcctagcatc gcgataagct ctagagggac agcccccccc
     2281 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     2341 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     2401 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     2461 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     2521 gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta gagcttgata
     2581 tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca gggatgtaat
     2641 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     2701 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     2761 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     2821 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     2881 gcctgggcgc gcctggtggc gaacgatcaa cagacgggag attgccaagg tcttgacaaa
     2941 cgagtgctag tacaagatcg cgtcgacgga tcgggagatc tgaattcgga tccagagtga
     3001 ggactaccta tagaagtaag agtgaggaca atcgtataca agtagatcca gagtgaggac
     3061 tacctataga agtaagagtg aggacaatcg tatacaagta gatccagagt gaggactacc
     3121 tatagaagta agagtgagga caatcgtata caagtagatc cctctagagg gtatataatg
     3181 ggggccacta gtctactacc agagctcatc gctagcgcca ccatggtgag cgagctgatt
     3241 aaggagaaca tgcacatgaa gctgtatatg gagggcaccg tgaacaacca ccacttcaag
     3301 tgtacctccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggcg
     3361 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc
     3421 agcaaaacct tcatcaacca cacccagggc atccccgact tctttaagca gtccttcccc
     3481 gagggcttca catgggagag agtcaccaca tacgaagatg ggggcgtgct gaccgctacc
     3541 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
     3601 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc ctccaccgaa
     3661 accctgtacc ccgctgacgg cggcctggaa ggcagagccg acatggccct gaagctcgtg
     3721 ggcgggggcc acctgatctg caacttgaaa accacataca gatccaagaa acccgctaag
     3781 aacctcaaga tgcccggcgt ctactatgtg gacaggagac tggaaagaat caaggaggcc
     3841 gacaaagaaa cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct
     3901 agcaaactgg ggcacagatg agcggccgct cgagtctaga gggcccgttt aaacccgctg
     3961 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4021 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4081 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4141 gggggaggat tgggaagtca atagcaggca tgctggggat gcggtgggct ctatggcttc
     4201 tgaggcaggt cgagggtaca tgcgccttac tccttgtgag cgcatcgcga taagctctag
     4261 agggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg
     4321 cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg
     4381 gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac
     4441 gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa aagctttagg
     4501 ctgaaagaga gatttagaat gacagaatca tagaacggcc tgcgtacgga tggggctcta
     4561 gctctagagc ttgatatcga attcctgcag ccccgctaga gggacagccc ccccccaaag
     4621 cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc
     4681 tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg
     4741 ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc
     4801 ctgcagacac ctggggggat acggggaaaa agctttaggc tgaaagagag atttagaatg
     4861 acagaatcat agaacggcct gggcgcgcct ggtggcgaac gatcaacaga cgggagaggg
     4921 caaaaccgat gttgacggac taatcctgac aagatctggg gaggtcgctg agtagtgcgc
     4981 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     5041 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gtgagtaatt
     5101 catacaaaag gactcgcccc tgccttgggg aatcccaggg accgtcgtta aactcccact
     5161 aacgtagaac ccagagatcg ctgcgttccc gccccctcac ccgcccgctc tcgtcatcac
     5221 tgaggtggag aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg
     5281 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     5341 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     5401 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     5461 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
     5521 tatggccctt gcgtgccttg aattacttcc acgcccctgg ctgcagtacg tgattcttga
     5581 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
     5641 ttcgcctcgt gcttgagttg aggcctggct tgggcgctgg ggccgccgcg tgcgaatctg
     5701 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     5761 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
     5821 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     5881 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     5941 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     6001 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     6061 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     6121 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     6181 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     6241 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     6301 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     6361 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     6421 gtcgtgagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     6481 gacctccata gaagagaacg ggaccgatcc aaattctcga ggctagcgcc accatggtga
     6541 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     6601 taaacggcca caagttcagc gtgaggggcg agggcgaggg cgatgccacc aacggcaagc
     6661 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     6721 ccaccctgag ccacggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     6781 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     6841 acgacggcac ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctagtgaacc
     6901 gcatcgagct gaagggcgtc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     6961 agtacaactt caacagccac aacatctata tcatggccgt caagcagaag aacggcatca
     7021 aggtgaactt caagatccgc cacaacgtgg aggacggcag cgtgcagctc gccgaccact
     7081 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacagc cactacctga
     7141 gcacccagtc cgtgctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     7201 agttccgcac cgccgccggg atcactctcg gcatggacga gctgtacaag gaattcggta
     7261 gcaagcttgg tagtggagca actaactttt ccctgctcaa acaagccggg gatgtagagg
     7321 agaaccccgg ccccgccccc cttaagatgg ccaagccttt gtctcaagaa gaatccaccc
     7381 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaggac tacagcgtcg
     7441 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     7501 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     7561 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     7621 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     7681 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     7741 gctaagcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
     7801 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     7861 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     7921 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     7981 gtcaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc aggtcgaaga
     8041 actatttcct ggctgttacg cgagcgcatc gcgataagct ctagagggac agcccccccc
     8101 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     8161 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     8221 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     8281 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     8341 gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta gagcttgata
     8401 tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca gggatgtaat
     8461 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     8521 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     8581 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     8641 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     8701 gcctgggcgc gcctggtggc gaacgatcaa cagacgggag aggactagag gatgcacatg
     8761 tgaccgaggg atggaacgac gcgtaactca cgttaaggga ttttggtcat gagcttgcgc
     8821 cgtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt
     8881 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     8941 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     9001 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     9061 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     9121 aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc
     9181 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
     9241 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
     9301 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
     9361 cttctaatac ctggaatgct gtttttccgg ggatcgcagt ggtgagtaac catgcatcat
     9421 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
     9481 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
     9541 actctggcgc atcgggcttc ccatacaagc gatagattgt cgcacctgat tgcccgacat
     9601 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     9661 tcgacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag
     9721 acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt
     9781 gagacacggg ccatgctagc tccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9841 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9901 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9961 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    10021 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    10081 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    10141 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    10201 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    10261 gtggcctaac tac
//
