LOCUS       sequence-319172-         810 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Codon-optimized bacterial expression plasmid. Expresses 
            GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Elias RD, Ramaraju B, Deshmukh L
  TITLE     Mechanistic Roles of Tyrosine Phosphorylation in Reversible 
            Amyloids, Autoinhibition, and Endosomal Membrane Association of 
            ALIX.
  JOURNAL   J Biol Chem. 2021 Oct 21:101328. doi: 10.1016/j.jbc.2021.101328.
  PUBMED    34688656
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2021 Oct 21:101328. doi: 10.1016/j.jbc.2021.101328."
COMMENT     Sequence Label: GB1-PRD(703-868, S712C, P801G, C813S)-Strep
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             172..189
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             190..201
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             241..258
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             259..279
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             778..801
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 catatgcaat acaaactggc gctgaacggc aaaacgctga agggtgaaac gacgaccgag
       61 gccgttgatg cggccacggc cgagaaagtg ttcaagcagt acgccaacga caacggtgtt
      121 gatggcgaat ggacctacga cgatgcgacc aaaaccttca ccgtgaccga actggttccg
      181 cgcggcagta tcgaaggtcg catccgcgag aatctgaaag atagcggtct gtttctggag
      241 catcaccacc atcaccatga aaatctgtac ttccaaggcg acgaactgct gaaagatctg
      301 cagcagtgca ttgcccgcga accaagcgcc ccaagcatcc cgacgccagc ctatcagagt
      361 agtccggcgg gtggtcatgc gccgacccca ccaacgccgg ccccacgtac gatgccgcca
      421 accaaaccgc aaccaccagc gcgtccgcca ccaccagttc tcccagccaa tcgcgcccca
      481 agtgcgaccg cgccaagccc agttggtgcg ggtaccgccg cgccagcgcc aagtcaaacg
      541 ccgggtagtg ccccaccacc acaagcgcaa ggcggcccat atccgacgta cccgggctat
      601 ccgggctaca gtcagatgcc aatgccgatg ggttacaacc catacgcgta cggccagtat
      661 aacatgccgt acccaccggt gtaccaccag agtccgggcc aagccccata cccgggtcca
      721 cagcagccaa gctacccgtt cccgcagcca ccacagcaaa gttactaccc gcagcaatgg
      781 agccatccgc agttcgaaaa gtaaggatcc
//
