LOCUS       sequence-319468-       10144 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Integration Vector, assembled with pHIE044 (TUPV1), pJM451 (TUPV2), 
            pPD612.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 10144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pHIE060 ZF43x3-C mKate2 EF1a EBFP2-P2A-BlastR
FEATURES             Location/Qualifiers
     source          1..10144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1031..1068
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1079..1678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1079..1098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1575..1595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1791..1810)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1856..1911
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1910..1929)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      3088..3097
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3094..3792
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3843..4067
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5116..6298
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5347..6289
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6246..6266
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     6300..6324
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6399..6408
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6405..6407,6408..6410,6411..7121)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(6450..6471)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             7149..7205
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7218..7616
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7667..7891
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(8750..9559)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9473..9492
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9551..9570
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(join(9718..10144,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9798..9817)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      241 ttatcaaaaa ggatcttcac ctagatcctt tctcgagctt tcggagtgag atggagtggc
      301 cactgtggaa ctgggcctag catcgcgata agctctagag ggacagcccc cccccaaagc
      361 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      421 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      541 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
      601 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
      661 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
      961 gcgcgcctgg tggcgaacga tcaacagacg ggagatctac tattgggaca gcagagatcc
     1021 agttggtaat ggcttgtcga cgacggcggt ctccgtcgtc aggatcatag gtgccaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc
     1681 ttccactgga ttgtacaatt accaactgaa ttcactcctc aggtgcaggc tgcctatcag
     1741 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     1801 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     1861 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1921 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1981 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     2041 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     2101 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     2161 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     2221 cttctcttat ggagatccgg gcctagcatc gcgataagct ctagagggac agcccccccc
     2281 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     2341 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     2401 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     2461 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     2521 gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta gagcttgata
     2581 tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca gggatgtaat
     2641 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     2701 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     2761 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     2821 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     2881 gcctgggcgc gcctggtggc gaacgatcaa cagacgggag attgccaagg tcttgacaaa
     2941 cgagtgctag tacaagatcg cgtcgacgga tcgggagatc tgaattagag tgaggacgat
     3001 cggagtgagg ataattagag tgaggacgat ccctctagag ggtatataat gggggccact
     3061 agtctactac cagagctcat cgctagcgcc accatggtga gcgagctgat taaggagaac
     3121 atgcacatga agctgtatat ggagggcacc gtgaacaacc accacttcaa gtgtacctcc
     3181 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggc ggtcgagggc
     3241 ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcaaaacc
     3301 ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc cgagggcttc
     3361 acatgggaga gagtcaccac atacgaagat gggggcgtgc tgaccgctac ccaggacacc
     3421 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc
     3481 aacggccctg tgatgcagaa gaaaacactc ggctgggagg cctccaccga aaccctgtac
     3541 cccgctgacg gcggcctgga aggcagagcc gacatggccc tgaagctcgt gggcgggggc
     3601 cacctgatct gcaacttgaa aaccacatac agatccaaga aacccgctaa gaacctcaag
     3661 atgcccggcg tctactatgt ggacaggaga ctggaaagaa tcaaggaggc cgacaaagaa
     3721 acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg
     3781 gggcacagat gagcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
     3841 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     3901 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     3961 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     4021 ttgggaagtc aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcagg
     4081 tcgagggtac atgcgcctta ctccttgtga gcgcatcgcg ataagctcta gagggacagc
     4141 ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga
     4201 gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc
     4261 ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc
     4321 tgctctttga gcctgcagac acctgggggg atacggggaa aaagctttag gctgaaagag
     4381 agatttagaa tgacagaatc atagaacggc ctgcgtacgg atggggctct agctctagag
     4441 cttgatatcg aattcctgca gccccgctag agggacagcc cccccccaaa gcccccaggg
     4501 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     4561 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     4621 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     4681 cctgggggga tacggggaaa aagctttagg ctgaaagaga gatttagaat gacagaatca
     4741 tagaacggcc tgggcgcgcc tggtggcgaa cgatcaacag acgggagagg gcaaaaccga
     4801 tgttgacgga ctaatcctga caagatctgg ggaggtcgct gagtagtgcg cgagcaaaat
     4861 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     4921 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgagtaat tcatacaaaa
     4981 ggactcgccc ctgccttggg gaatcccagg gaccgtcgtt aaactcccac taacgtagaa
     5041 cccagagatc gctgcgttcc cgccccctca cccgcccgct ctcgtcatca ctgaggtgga
     5101 gaagagcatg cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     5161 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     5221 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     5281 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     5341 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     5401 tgcgtgcctt gaattacttc cacgcccctg gctgcagtac gtgattcttg atcccgagct
     5461 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     5521 tgcttgagtt gaggcctggc ttgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     5581 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     5641 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     5701 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     5761 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     5821 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     5881 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     5941 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     6001 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     6061 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     6121 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     6181 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     6241 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaga
     6301 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     6361 agaagagaac gggaccgatc caaattctcg aggctagcgc caccatggtg agcaagggcg
     6421 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     6481 acaagttcag cgtgaggggc gagggcgagg gcgatgccac caacggcaag ctgaccctga
     6541 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     6601 gccacggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     6661 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     6721 cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctagtgaac cgcatcgagc
     6781 tgaagggcgt cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     6841 tcaacagcca caacatctat atcatggccg tcaagcagaa gaacggcatc aaggtgaact
     6901 tcaagatccg ccacaacgtg gaggacggca gcgtgcagct cgccgaccac taccagcaga
     6961 acacccccat cggcgacggc cccgtgctgc tgcccgacag ccactacctg agcacccagt
     7021 ccgtgctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttccgca
     7081 ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggaattcggt agcaagcttg
     7141 gtagtggagc aactaacttt tccctgctca aacaagccgg ggatgtagag gagaaccccg
     7201 gccccgcccc ccttaagatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
     7261 gagcaacggc tacaatcaac agcatcccca tctctgagga ctacagcgtc gccagcgcag
     7321 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
     7381 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
     7441 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
     7501 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
     7561 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcgg
     7621 ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     7681 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     7741 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     7801 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agtcaatagc
     7861 aggcatgctg gggatgcggt gggctctatg gcttctgagg caggtcgaag aactatttcc
     7921 tggctgttac gcgagcgcat cgcgataagc tctagaggga cagccccccc ccaaagcccc
     7981 cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg
     8041 ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca
     8101 cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc
     8161 agacacctgg ggggatacgg ggaaaaagct ttaggctgaa agagagattt agaatgacag
     8221 aatcatagaa cggcctgcgt acggatgggg ctctagctct agagcttgat atcgaattcc
     8281 tgcagccccg ctagagggac agcccccccc caaagccccc agggatgtaa ttacgtccct
     8341 cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc
     8401 gcatccccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc
     8461 gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg gggatacggg
     8521 gaaaaagctt taggctgaaa gagagattta gaatgacaga atcatagaac ggcctgggcg
     8581 cgcctggtgg cgaacgatca acagacggga gaggactaga ggatgcacat gtgaccgagg
     8641 gatggaacga cgcgtaactc acgttaaggg attttggtca tgagcttgcg ccgtcccgtc
     8701 aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact
     8761 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     8821 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     8881 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     8941 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     9001 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     9061 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     9121 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     9181 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     9241 cctggaatgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     9301 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     9361 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     9421 catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     9481 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgacgttt
     9541 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     9601 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacgg
     9661 gccatgctag ctccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     9721 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9781 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9841 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9901 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9961 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    10021 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    10081 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    10141 ctac
//
