LOCUS       sequence-319566-        9664 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Overexpresses ASIC4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9664)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_ASIC4_OE_1
FEATURES             Location/Qualifiers
     source          1..9664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2498..2522)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2524..2565
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2607..3195
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2660..2680)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3078..3089)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3267..3500
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3578..3699
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3615..3634)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3669..3688
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3739..3874
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3894..3913)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3895..3913)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3923..3940)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3923..3939)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3932..3954)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4081..4536
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4168..4187)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4378..4399
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4562..4666
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4667..4735,4736..5527)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4885..4904)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5698..6286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6187..6206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6574..6595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6610..6615,6616..6633,6634..6640)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6648..6664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6653..6675
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6672..6688
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6672..6688
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6707..6727
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6709..6727
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6755..6981
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6982..7162
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7209..7334
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7827..8060
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8245..8289
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8464..8964
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8883..8901
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8976..9371
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9431..9548
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagc aggctatgct gagcggagcg gctggggctg cgcggcgtgg cggagcagcg
      601 ctcgctccct cgctcactcg ctcgctcgca gggacacacg caggggctga cagctgtgct
      661 ggtgctgata agggaagcca caaggagacg atcgaggaga gagacaagcg gcagcagagg
      721 cagcagcggc agaggcagca ccagggctgc ggagctgctg ggagtgggag tgactccccc
      781 acctcgggcc cccaccctgt ccctgtcctc ttcccgcttg ccctgagttt agaagagcag
      841 ccgctgccac cactgccact cgggagggca ccagggctgc tggctaggga gggacagggc
      901 agggaggctc tggccagtcc cagcagccgg ggacagatgc cgatcgagat tgtgtgcaaa
      961 atcaaatttg ctgaggagga tgcgaaaccc aaggagaagg aggcagggga tgagcagagc
     1021 ctcctcgggg ctgttgcccc tggagcagcc ccccgagacc tggccacctt tgccagcacc
     1081 agcaccctgc atggactggg ccgggcctgt ggcccaggcc cccacggact gcgcagaacc
     1141 ctgtgggcac tggccctact cacctcgctg gctgccttcc tgtaccaggc ggctggcctg
     1201 gcccggggct acctgacccg gcctcacctg gtggcaatgg accccgctgc cccagcccca
     1261 gtggcgggct tcccggctgt caccctctgc aatatcaacc gcttccggca ttcggcactc
     1321 agcgatgccg acatcttcca cctggccaat ctgacagggc tgccccccaa agaccgggat
     1381 gggcaccgtg cggctggcct gcgctaccca gagcctgaca tggtagacat cctcaaccgc
     1441 actggccacc agctcgccga catgcttaag agctgcaact tcagtgggca tcactgctcc
     1501 gccagcaact tctctgtggt ctatactcgc tatgggaagt gttacacctt caacgcggac
     1561 ccgcggagct cgctgcccag ccgggcaggg ggcatgggca gtggcctgga gatcatgctg
     1621 gacatccagc aggaggagta cctgcccatc tggagggaga caaatgagac gtcgtttgag
     1681 gcaggtattc gggtgcagat ccacagccag gaggagccgc cctacatcca ccagctgggg
     1741 ttcggggtgt ccccaggctt ccagaccttt gtgtcctgcc aggaacagcg gctgacctac
     1801 ctgccccagc cctggggcaa ctgccgcgca gagagtgagc tcagggagcc tgagcttcag
     1861 ggctactcgg cctacagtgt gtctgcctgc cggctgcgct gtgaaaagga ggccgtgctt
     1921 cagcgctgcc actgccggat ggtgcacatg ccagactccc tgggtggggg ccctgagggc
     1981 ccgtgcttct gccccacccc ctgcaacctg acacgctatg ggaaagagat ctccatggtc
     2041 aggatcccca acaggggctc agcccggtac ctggcgagga agtacaaccg caacgagacc
     2101 tacatacggg agaacttcct ggtcctagat gtcttctttg aggccctgac ctctgaagcc
     2161 atggagcagc gagcagccta tggcctgtca gccctgctgg gagacctcgg gggacagatg
     2221 ggcctgttca ttggggccag catcctcacg ttgctggaga tcctcgacta catctatgag
     2281 gtgtcctggg atcgactgaa gcgggtatgg aggcgtccca agacccccct gcggacctcc
     2341 actgggggca tctccacttt ggggcttcag gagctgaagg aacagagtcc ctgcccgagc
     2401 ctgggccgag cggagggtgg gggggtcagc agtctgctcc ccaatcacca ccacccccac
     2461 ggtcccccag gaggtctctt tgaagatttt gcttgctacc cagctttctt gtacaaagtg
     2521 gttggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc
     2581 gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     2641 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     2701 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     2761 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     2821 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     2881 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     2941 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     3001 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     3061 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     3121 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     3181 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag
     3241 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     3301 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     3361 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     3421 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     3481 agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt
     3541 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     3601 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3661 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     3721 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     3781 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     3841 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     3901 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     3961 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4021 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     4081 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4141 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4201 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4261 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4321 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4381 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4441 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4501 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     4561 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4621 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4681 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4741 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4801 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     4861 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4921 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     4981 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5041 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5101 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5161 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5221 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5281 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5341 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5401 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5461 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5521 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5581 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5641 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5701 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5761 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5821 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     5881 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5941 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6001 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6061 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6121 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6181 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6241 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6301 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6361 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6421 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     6481 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     6541 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     6601 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     6661 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     6721 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     6781 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     6841 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     6901 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     6961 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     7021 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7081 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7141 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     7201 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     7261 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     7321 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     7381 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     7441 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     7501 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     7561 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     7621 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     7681 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     7741 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     7801 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     7861 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     7921 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     7981 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     8041 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     8101 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     8161 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     8221 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     8281 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     8341 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     8401 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     8461 cccttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     8521 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     8581 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     8641 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     8701 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     8761 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     8821 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     8881 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     8941 cgaatcaccg acctctctcc ccagggggta ccaccatggc caagcctttg tctcaagaag
     9001 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     9061 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     9121 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
     9181 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     9241 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
     9301 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     9361 tgtgggaggg cctgcagctg cagtagtaag aattctagat cttgagacaa atggcagtat
     9421 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     9481 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     9541 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggggg
     9601 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     9661 tccg
//
