LOCUS       sequence-319660-        9114 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Overexpresses GLRA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9114)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_GLRA3_OE_1
FEATURES             Location/Qualifiers
     source          1..9114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        37..340
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        341..544
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     495..515
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    567..591
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1984..2008)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2010..2051
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2093..2681
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2146..2166)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2564..2575)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2753..2986
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3064..3185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3101..3120)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3155..3174
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3225..3360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3380..3399)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3381..3399)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3409..3426)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3409..3425)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3418..3440)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3567..4022
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3654..3673)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3864..3885
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4048..4152
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4153..4221,4222..5013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4371..4390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5184..5772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5673..5692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5926..5943
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6060..6081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6096..6101,6102..6119,6120..6126)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6134..6150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6139..6161
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6158..6174
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6158..6174
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6193..6213
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6195..6213
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6241..6467
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6468..6648
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6695..6820
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7313..7546
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7731..7775
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7950..8450
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8369..8387
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8462..8857
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8917..9034
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
       61 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      121 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      181 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      241 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      301 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      361 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      421 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      481 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      541 agctctctgg ctaactgtcg ggatcaacaa gtttgtacaa aaaagcaggc accatggccc
      601 acgtgagaca ctttcggaca ttagtttcgg gattttactt ctgggaagca gcactgttac
      661 tcagtttggt tgccacaaag gaaacagaca gtgcaagatc tcgaagtgct ccaatgtcac
      721 cttctgattt tctggataaa ttaatgggca ggacatcagg atatgatgca agaatcagac
      781 ccaattttaa aggccctcca gttaatgtca catgcaacat attcatcaac agctttggct
      841 ctatcgcaga gacgaccatg gattacagag tgaatatctt tcttcgtcag aaatggaatg
      901 atccccgcct cgcgtacagt gaatatcctg acgactcttt agacctcgac ccctccatgt
      961 tggactccat ttggaaacct gatttgttct ttgccaatga aaagggtgcc aactttcatg
     1021 aagtcactac agacaacaaa ttgctaagaa ttttcaaaaa tggaaatgtt ctttattcaa
     1081 taagattaac attaacactt tcctgtccaa tggatctcaa gaattttccc atggatgtac
     1141 aaacatgtat aatgcaactg gaaagctttg ggtacacaat gaatgatctc atttttgaat
     1201 ggcaagatga ggcacccgta caagtggcag aaggactcac tttgccccag tttctgttga
     1261 aagaagaaaa agatttacga tactgcacta aacattacaa tacaggaaag tttacgtgta
     1321 tagaagtgcg attccatctg gagcgacaaa tgggatacta tctgatccag atgtacattc
     1381 ccagtctcct gattgttatt ctatcctggg tttcgttctg gatcaacatg gatgcagcac
     1441 cggccagggt agctctgggg ataaccaccg tgctaacgat gactacacag agttcaggat
     1501 cacgagcttc cttgccaaaa gtttcatatg tcaaagctat tgatatttgg atggcagtat
     1561 gcctcctttt tgtgttttca gcacttctgg agtatgcagc tgtaaatttt gtatcaagac
     1621 aacacaaaga acttctgaga tttcgacgaa agagaaagaa taagacagaa gcctttgcac
     1681 tggagaagtt ttaccgtttc tcagatatgg atgatgaggt aagggaaagc cgattcagct
     1741 tcacagccta tggaatggga ccatgtctac aagcaaagga tggcatgact ccaaagggcc
     1801 ccaaccaccc tgtccaggta atgccaaaaa gtcctgatga aatgaggaag gtctttatcg
     1861 accgggccaa gaagattgat accatctccc gagcctgctt cccattagct tttttgattt
     1921 ttaatatttt ctactgggtt atctataaaa ttcttaggca tgaggatatt catcagcagc
     1981 aagatacagc tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
     2041 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
     2101 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     2161 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     2221 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     2281 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     2341 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     2401 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     2461 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     2521 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     2581 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     2641 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg
     2701 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     2761 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     2821 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2881 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2941 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt
     3001 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     3061 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3121 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3181 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     3241 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3301 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3361 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     3421 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     3481 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     3541 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     3601 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3661 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3721 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3781 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3841 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     3901 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     3961 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     4021 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4081 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4141 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4201 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4261 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4321 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4381 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4441 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4501 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4561 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4621 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4681 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4741 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4801 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4861 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4921 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4981 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5041 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5101 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5161 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5221 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5281 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5341 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5401 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5461 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5521 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5581 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5641 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5701 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5761 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5821 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5881 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     5941 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     6001 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     6061 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     6121 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     6181 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     6241 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     6301 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     6361 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     6421 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     6481 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6541 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6601 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     6661 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     6721 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     6781 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     6841 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     6901 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     6961 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     7021 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     7081 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     7141 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     7201 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     7261 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     7321 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     7381 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     7441 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     7501 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     7561 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     7621 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     7681 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     7741 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     7801 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     7861 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     7921 agacccacct cccaaccccg aggggaccct tgcgcctttt ccaaggcagc cctgggtttg
     7981 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     8041 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     8101 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     8161 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     8221 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     8281 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     8341 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     8401 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggtaccac
     8461 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     8521 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     8581 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     8641 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     8701 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     8761 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     8821 tcgtgaattg ctgccctctg gttatgtgtg ggagggcctg cagctgcagt agtaagaatt
     8881 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     8941 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     9001 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     9061 tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa ttac
//
