LOCUS       sequence-319665-        9117 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Overexpresses GABRG1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9117)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_GABRG1_OE_1
FEATURES             Location/Qualifiers
     source          1..9117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1951..1975)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1977..2018
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2060..2648
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2113..2133)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2531..2542)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2720..2953
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3031..3152
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3068..3087)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3122..3141
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3192..3327
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3347..3366)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3348..3366)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3376..3393)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3376..3392)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3385..3407)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3534..3989
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3621..3640)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3831..3852
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4015..4119
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4120..4188,4189..4980)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4338..4357)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5151..5739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5640..5659
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5893..5910
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6027..6048
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6063..6068,6069..6086,6087..6093)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6101..6117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6106..6128
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6125..6141
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6125..6141
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6160..6180
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6162..6180
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6208..6434
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6435..6615
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6662..6787
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7280..7513
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7698..7742
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7917..8417
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8336..8354
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8429..8824
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8884..9001
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagc aggcaccatg ggtcctttga aagcttttct cttctcccct tttcttctgc
      601 ggagtcaaag tagaggggtg aggttggtct tcttgttact gaccctgcat ttgggaaact
      661 gtgttgataa ggcagatgat gaagatgatg aggatttaac ggtgaacaaa acctgggtct
      721 tggccccaaa aattcatgaa ggagatatca cacaaattct gaattcattg cttcaaggct
      781 atgacaataa acttcgtcca gatataggag tgaggcccac agtaattgaa actgatgttt
      841 atgtaaacag cattggacca gttgatccaa ttaatatgga atatacaata gatataattt
      901 ttgcccaaac ctggtttgac agtcgtttaa aattcaatag taccatgaaa gtgcttatgc
      961 ttaacagtaa tatggttgga aaaatttgga ttcctgacac tttcttcaga aactcaagaa
     1021 aatctgatgc tcactggata acaactccta atcgtctgct tcgaatttgg aatgatggac
     1081 gagttctgta tactctaaga ttgacaatta atgcagaatg ttatcttcag cttcataact
     1141 ttcccatgga tgaacattcc tgtccactgg aattttcaag ctatggatac cctaaaaatg
     1201 aaattgagta taagtggaaa aagccctccg tagaagtggc tgatcctaaa tactggagat
     1261 tatatcagtt tgcatttgta gggttacgga actcaactga aatcactcac acgatctctg
     1321 gggattatgt tatcatgaca attttttttg acctgagcag aagaatggga tatttcacta
     1381 ttcagaccta cattccatgc attctgacag ttgttctttc ttgggtgtct ttttggatca
     1441 ataaagatgc agtgcctgca agaacatcgt tgggtatcac tacagttctg actatgacaa
     1501 ccctgagtac aattgccagg aagtctttac ctaaggtttc ttatgtgact gcgatggatc
     1561 tctttgtttc tgtttgtttc atttttgttt ttgcagcctt gatggaatat ggaaccttgc
     1621 attattttac cagcaaccaa aaaggaaaga ctgctactaa agacagaaag ctaaaaaata
     1681 aagcctcgat gactcctggt ctccatcctg gatccactct gattccaatg aataatattt
     1741 ctgtgccgca agaagatgat tatgagtatc agtgtttgga gggcaaagat tgtgccagct
     1801 tcttctgttg ctttgaagac tgcagaacag gatcttggag ggaaggaagg atacacatac
     1861 gcattgccaa aattgactct tattctagaa tatttttccc aaccgctttt gccctgttca
     1921 acttggttta ttgggttggc tatctttact taacagcttt cttgtacaaa gtggttggta
     1981 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
     2041 ggtggcgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2101 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2161 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     2221 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     2281 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     2341 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     2401 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     2461 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     2521 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     2581 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     2641 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     2701 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     2761 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     2821 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     2881 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     2941 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     3001 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     3061 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3121 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     3181 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3241 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3301 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     3361 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3421 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3481 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     3541 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3601 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3661 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3721 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     3781 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3841 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     3901 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3961 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     4021 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4081 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4141 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4201 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4261 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4321 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4381 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4441 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4501 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4561 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4621 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4681 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4741 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4801 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4861 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4921 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4981 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5041 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5101 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5161 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5221 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5281 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     5341 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5401 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5461 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5521 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5581 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5641 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5701 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5761 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5821 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5881 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     5941 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6001 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     6061 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     6121 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     6181 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     6241 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     6301 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     6361 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     6421 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6481 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6541 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6601 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6661 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     6721 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     6781 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     6841 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     6901 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     6961 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     7021 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     7081 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     7141 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     7201 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     7261 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     7321 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     7381 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     7441 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     7501 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     7561 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     7621 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     7681 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     7741 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     7801 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     7861 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccttgc
     7921 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     7981 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     8041 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     8101 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     8161 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     8221 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     8281 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     8341 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     8401 ccgacctctc tccccagggg gtaccaccat ggccaagcct ttgtctcaag aagaatccac
     8461 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     8521 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     8581 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     8641 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     8701 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     8761 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     8821 gggcctgcag ctgcagtagt aagaattcta gatcttgaga caaatggcag tattcatcca
     8881 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     8941 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     9001 tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg gggcccgggg
     9061 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccg
//
