LOCUS       sequence-319872-         714 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3547
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(135..176)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    184..208
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgac ttaacgcgtc gatagggaat cccaggatcg
       61 ggttcgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacgga
      121 ctagttcatt actacgtaga atcgagaccg aggagagggt tagggatagg cttaccgata
      181 tcaaccactt tgtacaagaa agttgggtag ggtcttgttg tcttcttgcc agttactgcc
      241 agcagctgtg aaactctcac ctccatttcc tcccgatgag acctgtctct atcttcaaat
      301 tcgcgtgtcc ttctccgagc ctcttcaaag gcctgttgtg ctaatgcatc ctcctgctgg
      361 gctcgctcct catcaaactc cagacagccc ataccatcca gtctttggag atcaatccag
      421 cctttccaga taacacagac tccattcaga atcatgggag ccttcaaata taccttgcct
      481 gcttctcttt ctaagtcgac atactctcgg ctaggtgttt gtcgctgttc tccctgccag
      541 ctggccggaa aaaactggag agacagattg gttcctgtgg ccacaaaagc gatttccgag
      601 cggccgtcgc ggcaggcgtt ctggaagcgt gcctggcgtt cctcgtgggg ccggtccctg
      661 aagcccgtgt acttaatctc acactcgcgg ctcagcttcc taaaaaactc ctcg
//
