LOCUS       sequence-319924-         806 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3541
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(137..178)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    186..210
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 caaattttgt aatccagagg ttgattgtcg acttaacgcg tggccgcggg cctatatcct
       61 caaagtcgtc ctttccacaa gatatataaa gccaagaaat cgaaatactt tcaagttacg
      121 gactagttca ttactacgta gaatcgagac cgaggagagg gttagggata ggcttaccga
      181 tatcaaccac tttgtacaag aaagttgggc atgaaatata gtcctgttct atgctctcct
      241 gacaacagtc ccaacagaaa gtgtccagtc tatttgctgt ttcttggctg atcagacatt
      301 catcctttaa ttccctgatt gtcttcctca atgatttctg aaaacgtttt tctcggtcta
      361 aaacattccg tagactttcc tttgtatcct ctagctcgcc gatcacatga tctaatttat
      421 gcttaagttt ctttggactg tccattacac tgtagctatg ttcaaaaatg aactgggatt
      481 ggaattcttc aatacatgag gaagcaccaa caagatggtg attgtagtta gtggctggaa
      541 cggttttgag ggtgaagttt tttctacaat gaagtttttc tcttttcccc tgtaggtgat
      601 atggagaatc aaagatagaa ggtatgactc caggtttcag tttaatattt ggagcacttc
      661 tgtcaaaatc tgtcttctta aagtgcctcg aacacaacac atctcctttt ttaggctccc
      721 aaatgccggc tgcattcaca tcaagtcttt tcattgctaa tacccatttc cttttgatgt
      781 tttcatctgt ggggaatacg tgaaat
//
