LOCUS       sequence-320087-        9998 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses a sgRNA targeting mouse Htt promoter for gene activation 
            using the SAM system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9998)
  AUTHORS   Shen M, Wang F, Li M, Sah N, Stockton ME, Tidei JJ, Gao Y, 
            Korabelnikov T, Kannan S, Vevea JD, Chapman ER, Bhattacharyya A, van
            Praag H, Zhao X
  TITLE     Reduced mitochondrial fusion and Huntingtin levels contribute to 
            impaired dendritic maturation and behavioral deficits in Fmr1-mutant
            mice.
  JOURNAL   Nat Neurosci. 2019 Mar;22(3):386-400. doi: 
            10.1038/s41593-019-0338-y. Epub 2019 Feb 11.
  PUBMED    30742117
REFERENCE   2  (bases 1 to 9998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-019-0338-y"; journalName: "Nat Neurosci"; date: 
            "2019-03"; volume: "22"; issue: "3"; pages: "386-400"
COMMENT     Sequence Label: 61427-sgHtt_6
FEATURES             Location/Qualifiers
     source          1..9998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        181..228
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             247..621
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    751..872
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(788..807)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     842..861
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(921..937)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(921..937)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(934..956)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    945..961
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(969..975,976..993,994..999))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1014..1035
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1152..1169)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1323..1911)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1403..1422)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2082..2873,2874..2942))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2705..2724
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2943..3047)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3122..3141)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3313..3692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3694..3892
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3847..3867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3910..4090
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4137..4262
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4755..4988
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5173..5217
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5515..5632
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5682..5930
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5682..5702
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5853..5872
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5969..5987
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        6039..6057
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        6181..7359
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6412..7350
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7307..7327
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7366..7740
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    7765..8353
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7818..7838)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8236..8247)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8356..8372)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8357..8373)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8878..9058
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9084..9101)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9090..9314
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9360..9788
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9447..9466)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9657..9678
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9797..9817)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(9802..9998,1..133)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(9982..9998,1..119)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
       61 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
      121 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
      181 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
      241 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
      301 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
      361 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
      421 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
      481 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
      541 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
      601 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
      661 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
      721 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
      781 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
      841 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
      901 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      961 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     1021 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     1081 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     1141 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1201 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1261 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1321 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1381 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1441 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1501 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1561 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1621 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1681 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1741 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1801 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1861 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1921 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1981 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2041 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2101 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     2161 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     2221 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     2281 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     2341 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     2401 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     2461 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     2521 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     2581 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     2641 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     2701 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2761 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     2821 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2881 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     2941 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3001 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3061 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     3121 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     3181 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     3241 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     3301 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     3361 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     3421 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     3481 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     3541 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     3601 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     3661 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     3721 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     3781 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     3841 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt
     3901 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     3961 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     4021 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     4081 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     4141 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     4201 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     4261 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     4321 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     4381 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     4441 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     4501 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     4561 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     4621 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     4681 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     4741 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     4801 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     4861 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     4921 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     4981 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     5041 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     5101 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     5161 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     5221 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     5281 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     5341 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     5401 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     5461 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     5521 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     5581 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     5641 ttacagggac agcagagatc cagtttggtt aattagctag cgagggccta tttcccatga
     5701 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga
     5761 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
     5821 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
     5881 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc gtcgtcctct
     5941 ttcccgaagt cgttttagag ctaggccaac atgaggatca cccatgtctg cagggcctag
     6001 caagttaaaa taaggctagt ccgttatcaa cttggccaac atgaggatca cccatgtctg
     6061 cagggccaag tggcaccgag tcggtgcttt ttttggatcc tgcaaagatg gataaagttt
     6121 taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt
     6181 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     6241 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     6301 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     6361 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     6421 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     6481 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     6541 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     6601 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     6661 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     6721 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     6781 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     6841 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     6901 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     6961 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatgaagg
     7021 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     7081 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     7141 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     7201 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     7261 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     7321 gtggttcaaa gtttttttct tccatttcag gtgtcgtgat gtacaatggc caagttgacc
     7381 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     7441 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     7501 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     7561 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     7621 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     7681 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     7741 gaattcgata tcaagcttat cggtaatcaa cctctggatt acaaaatttg tgaaagattg
     7801 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7861 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7921 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7981 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     8041 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     8101 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     8161 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     8221 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     8281 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     8341 gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca
     8401 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     8461 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     8521 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     8581 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
     8641 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
     8701 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
     8761 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
     8821 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
     8881 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8941 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     9001 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     9061 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     9121 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     9181 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     9241 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     9301 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     9361 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     9421 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     9481 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     9541 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     9601 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     9661 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     9721 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     9781 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     9841 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     9901 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     9961 gtcccgcccc taactccgcc catcccgccc ctaactcc
//
