LOCUS       sequence-320197-       12897 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses four gRNA targeting BTub gene with 3xp3-eGFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12897)
  AUTHORS   Li M, Yang T, Bui M, Gamez S, Wise T, Kandul NP, Liu J, Alcantara L,
            Lee H, Edula JR, Raban R, Zhan Y, Wang Y, DeBeaubien N, Chen J, 
            Sanchez C HM, Bennett JB, Antoshechkin I, Montell C, Marshall JM, 
            Akbari OS
  TITLE     Suppressing mosquito populations with precision guided sterile 
            males.
  JOURNAL   Nat Commun. 2021 Sep 10;12(1):5374. doi: 10.1038/s41467-021-25421-w.
  PUBMED    34508072
REFERENCE   2  (bases 1 to 12897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-09-10"; volume: "12(1):5374. doi"; pages: " 
            10.1038/s41467-021-25421-w"
COMMENT     Sequence Label: OA-1067A1
FEATURES             Location/Qualifiers
     source          1..12897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2998..3060
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      10737..10746
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(10743..10745,10746..10748,10749..11462)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(10743..10745,10746..10748,10749..11459)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10788..10809)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11049..11068)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11396..11417
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    11585..11706
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11622..11641)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11676..11695
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   12374..12408
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(12590..12606)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12590..12606)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12603..12625)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12614..12630
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12638..12644,12645..12662,12663..12668))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12683..12704
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12821..12838)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaatat aacaaaattg
     2341 tgatcccaca aaatgaagtg gggcaaaatc aaataattaa ctagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcg tgactataac acaagttgat
     2461 ttaataattt tagccaacac gtcgggctgc gtgttttttg cgctctgtgt acacgttgat
     2521 taactggtcg attaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt
     2581 taaaataact ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc
     2641 taatatgagg tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt
     2701 acacgaacgc gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg
     2761 caccatatac gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg
     2821 tgtctctgct caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca
     2881 agtcgatgag cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga
     2941 tatacggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
     3001 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
     3061 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     3121 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     3181 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
     3241 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
     3301 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
     3361 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
     3421 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
     3481 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
     3541 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
     3601 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt
     3661 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
     3721 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca
     3781 gaacacattt ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt
     3841 tgcggtttac cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat
     3901 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
     3961 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
     4021 tttgatcgca cggttcccac aatggttaat tcgagctcgc ccgggctcct aggagatctt
     4081 catcacaaac gaaactatta taatcgtctt caggggaacg gtagcatcat atctgtcacc
     4141 agtcagtcgg attcctttct ttcggcataa attatgctcg ggtgacgaga cgagttgaaa
     4201 atcccgtaaa ttaaaacata taccacactc gccgtcctag acgacgacga cgacgacgat
     4261 tcccaaggaa ttgaaccctt catttgcatt tcactaccgc tctgagagat gcaaagcgcg
     4321 ccagacagtc gatcgaatct gcagaccaga gagtcacatc aagaatgttg acattccacc
     4381 agaagactct aattattctt cgctcgactg ggaaaactgt taacaactgc tcgagtgtgg
     4441 tcggagcggc tcacagtgcg ttttcccgta gacaaaaatc aaatttcatg gtatttttcg
     4501 gcgataaaca gtaagttaaa gggaacccga atttagtgct atataattta attccactag
     4561 agtttgtatc ctttgataga tacgcgtatt tcgacctcaa ctgcaaggcc gtcgtgtact
     4621 agacttgact aatccagact ggtcttttag ttatgacttc tgtccacatc tccatacatt
     4681 caacgcactg tgcggctgtg ctgtgcgact ccgtcgagtc gaccaacata gttgaaacaa
     4741 attgaatatt taattgatcg ttataggaat ggtgttagat gagtcatcct ttacagtaag
     4801 cacatacagt attataattg aagatcgtcg gcagataggt gtgtagggta gagtatcagc
     4861 aataagttgg gacgtttgac tttttgtagg tagacaaaaa ctaaactttt tttcgcttct
     4921 ctatgtgtgc ccctgggtag cgttccgttc cgattggggt gcgaacgaat gaaatcgccc
     4981 atcgagttga tacgtccatc catcgctaga accgcgttcg ctgtagaaga ctatataaga
     5041 gcagaggcaa gagtagtgaa attactacaa cgaggctacc gggtcttaga gctatgctgg
     5101 aaacagcata gcaagttaag ataaggctag tccgttatca acttgaaaaa gtggcaccga
     5161 gtcggtgctt tttttggtgg ttgttcatcg attcctaata ttgcagtgaa gcagtttgtt
     5221 gtgcatggcg aagataaggt tagggcgagg aatgccttgg aaataagcgg cactgggccg
     5281 tatgaataaa aagcgttgaa ctttactaca tgtagcatct actcaaaaac aaaatccaca
     5341 aagtttacta cacttttggt atgaataaaa accttttcga acatgtagta cctactactt
     5401 taagaaaatt ctattcaaag tacagagtaa tgctgcatgt taagaaaatt ccattcagag
     5461 tgactcttga aatcaataaa atcatgcata tatgccaaat ttgtcactta ttcacgtgac
     5521 aaattatgtg aaaatatgaa tattactaca ttcccgctat gcatcgcgta atcgatcatg
     5581 aaatttatgt tggttatgta actttacagt attcccttta tgtgcatgtg aattttataa
     5641 tgatttgaca acaaggattt tccttagaat tttcgaattt tagctagaaa tctgtatcct
     5701 atgaatgaat cttgagataa cagcaattgt aacattataa agttcctaac attttcaaga
     5761 ttccagtatt gagtcgcgat cgcttaatta accgcggata tgtcttgccg aagcacatta
     5821 acccaagcca tttatatatc aagtacaagt tgatttgatt ctagtgatgg actgacacga
     5881 atctatggca ctgttgcaga gcttacaaac aggtacccgg agtcgcttca attattcaat
     5941 catccattca aaaacttcca tctcttccaa ttagccaata ttcaaattgg aatccagcca
     6001 gccgacggag gcagatggtc tacctggcaa ctatagagtt tccatgtttc cagactttcc
     6061 ctcccggtaa acggagacaa aacgacagac gtaagtaggt acatatgcat accgcacgga
     6121 caaatcaaat ttgtctggca gctccaatta gagtcgttaa aaatttaacg atgcgctaaa
     6181 taacttcaag ctatttgtct cgctggattg gcttcgagtg gtaagatcct atcaaatgcc
     6241 gaaaacaaaa aactttcttc ttaattgttc gttcttcaac acctctccat ggtgataacg
     6301 gatacggttt cattgtcagc atccatcctc cgaaaaatac attacgcctt gaaatatgca
     6361 atcgcaaaca cggatctgtt tggaacattt attttactat gaagagatgc gataggtaat
     6421 atttatttga gcgtttaaga tactcattgt tctctcaaag aatgtcattg aaagccaacg
     6481 aggtcaaatc aaatattata ataaaaaggt caaagaggac taacttaaag ctctctttat
     6541 ggataggaaa aaatattttc gcccatcgct agaactttta ccgtttccat tgagtatata
     6601 actaagatga atgaggctaa ttgatgtctc cgcaatacgc cccgggtctt agagctatgc
     6661 tggaaacagc atagcaagtt aagataaggc tagtccgtta tcaacttgaa aaagtggcac
     6721 cgagtcggtg ctttttttaa gatgcttcgg caattaataa taaggaatgg taaagcattc
     6781 ttcaaatgtt actatgttct caaattttca agaattaggt attcaagaaa ttctttcagg
     6841 catattccat aatctgcaca agcagctact ttagaaatta ttgccgggct taaattatat
     6901 gtacaacatt tcctgagtat tttctgacaa aaattctcac aagggaaggt cacgatggag
     6961 ttacacgggg tttttaaccg gactattttt gttagattct acatgtcgaa atatgaaaaa
     7021 ccccaaagcc tttcgggtga tggaccagtg gtgtagccag gaggggctct gaaaggggta
     7081 attttgtacg tatattatat tattaagcta attggaaatt tgacaaaaat atttttttag
     7141 tatttattgt gatggtagag aacaaaattg acattaatgt acgtttgaaa tgtatttgcc
     7201 atgcctttgg cgtactaaca tggagtatgg acaccaaaac cgatttcgct agcgtttaaa
     7261 ctcatcacaa acgaaactat tataatcgtc ttcaggggaa cggtagcatc atatctgtca
     7321 ccagtcagtc ggattccttt ctttcggcat aaattatgct cgggtgacga gacgagttga
     7381 aaatcccgta aattaaaaca tataccacac tcgccgtcct agacgacgac gacgacgacg
     7441 attcccaagg aattgaaccc ttcatttgca tttcactacc gctctgagag atgcaaagcg
     7501 cgccagacag tcgatcgaat ctgcagacca gagagtcaca tcaagaatgt tgacattcca
     7561 ccagaagact ctaattattc ttcgctcgac tgggaaaact gttaacaact gctcgagtgt
     7621 ggtcggagcg gctcacagtg cgttttcccg tagacaaaaa tcaaatttca tggtattttt
     7681 cggcgataaa cagtaagtta aagggaaccc gaatttagtg ctatataatt taattccact
     7741 agagtttgta tcctttgata gatacgcgta tttcgacctc aactgcaagg ccgtcgtgta
     7801 ctagacttga ctaatccaga ctggtctttt agttatgact tctgtccaca tctccataca
     7861 ttcaacgcac tgtgcggctg tgctgtgcga ctccgtcgag tcgaccaaca tagttgaaac
     7921 aaattgaata tttaattgat cgttatagga atggtgttag atgagtcatc ctttacagta
     7981 agcacataca gtattataat tgaagatcgt cggcagatag gtgtgtaggg tagagtatca
     8041 gcaataagtt gggacgtttg actttttgta ggtagacaaa aactaaactt tttttcgctt
     8101 ctctatgtgt gcccctgggt agcgttccgt tccgattggg gtgcgaacga atgaaatcgc
     8161 ccatcgagtt gatacgtcca tccatcgcta gaaccgcgtt cgctgtagaa gactatataa
     8221 gagcagaggc aagagtagtg aaatgatagg agccaagttc tggggtctta gagctatgct
     8281 ggaaacagca tagcaagtta agataaggct agtccgttat caacttgaaa aagtggcacc
     8341 gagtcggtgc tttttttggt ggttgttcat cgattcctaa tattgcagtg aagcagtttg
     8401 ttgtgcatgg cgaagataag gttagggcga ggaatgcctt ggaaataagc ggcactgggc
     8461 cgtatgaata aaaagcgttg aactttacta catgtagcat ctactcaaaa acaaaatcca
     8521 caaagtttac tacacttttg gtatgaataa aaaccttttc gaacatgtag tacctactac
     8581 tttaagaaaa ttctattcaa agtacagagt aatgctgcat gttaagaaaa ttccattcag
     8641 agtgactctt gaaatcaata aaatcatgca tatatgccaa atttgtcact tattcacgtg
     8701 acaaattatg tgaaaatatg aatattacta cattcccgct atgcatcgcg taatcgatca
     8761 tgaaatttat gttggttatg taactttaca gtattccctt tatgtgcatg tgaattttat
     8821 aatgatttga caacaaggat tttccttaga attttcgaat tttagctaga aatctgtatc
     8881 ctatgaatga atcttgagat aacagcaatt gtaacattat aaagttccta acattttcaa
     8941 gattccagta ttgagtcgcg atcgcttaat taaccgcgga tatgtcttgc cgaagcacat
     9001 taacccaagc catttatata tcaagtacaa gttgatttga ttctagtgat ggactgacac
     9061 gaatctatgg cactgttgca gagcttacaa acaggtaccc ggagtcgctt caattattca
     9121 atcatccatt caaaaacttc catctcttcc aattagccaa tattcaaatt ggaatccagc
     9181 cagccgacgg aggcagatgg tctacctggc aactatagag tttccatgtt tccagacttt
     9241 ccctcccggt aaacggagac aaaacgacag acgtaagtag gtacatatgc ataccgcacg
     9301 gacaaatcaa atttgtctgg cagctccaat tagagtcgtt aaaaatttaa cgatgcgcta
     9361 aataacttca agctatttgt ctcgctggat tggcttcgag tggtaagatc ctatcaaatg
     9421 ccgaaaacaa aaaactttct tcttaattgt tcgttcttca acacctctcc atggtgataa
     9481 cggatacggt ttcattgtca gcatccatcc tccgaaaaat acattacgcc ttgaaatatg
     9541 caatcgcaaa cacggatctg tttggaacat ttattttact atgaagagat gcgataggta
     9601 atatttattt gagcgtttaa gatactcatt gttctctcaa agaatgtcat tgaaagccaa
     9661 cgaggtcaaa tcaaatatta taataaaaag gtcaaagagg actaacttaa agctctcttt
     9721 atggatagga aaaaatattt tcgcccatcg ctagaacttt taccgtttcc attgagtata
     9781 taactaagat gaatgaggct aattgatcac tatggattcg gtccgaggtc ttagagctat
     9841 gctggaaaca gcatagcaag ttaagataag gctagtccgt tatcaacttg aaaaagtggc
     9901 accgagtcgg tgcttttttt aagatgcttc ggcaattaat aataaggaat ggtaaagcat
     9961 tcttcaaatg ttactatgtt ctcaaatttt caagaattag gtattcaaga aattctttca
    10021 ggcatattcc ataatctgca caagcagcta ctttagaaat tattgccggg cttaaattat
    10081 atgtacaaca tttcctgagt attttctgac aaaaattctc acaagggaag gtcacgatgg
    10141 agttacacgg ggtttttaac cggactattt ttgttagatt ctacatgtcg aaatatgaaa
    10201 aaccccaaag cctttcgggt gatggaccag tggtgtagcc aggaggggct ctgaaagggg
    10261 taattttgta cgtatattat attattaagc taattggaaa tttgacaaaa atattttttt
    10321 agtatttatt gtgatggtag agaacaaaat tgacattaat gtacgtttga aatgtatttg
    10381 ccatgccttt ggcgtactaa catggagtat ggacaccaaa accgatttcg gccttttggg
    10441 cccggcgcgc cgttcccaca atggttaatt cgagctcgcc cggggatcta attcaattag
    10501 agactaattc aattagagct aattcaatta ggatccaagc ttatcgattt cgaaccctcg
    10561 accgccggag tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc
    10621 aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta
    10681 aacaatcggg gtaccgctag agtcgacggt acccgcgggc ccgggatcca ccggtcgcca
    10741 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
    10801 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
    10861 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
    10921 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
    10981 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
    11041 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
    11101 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
    11161 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
    11221 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
    11281 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
    11341 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
    11401 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
    11461 aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
    11521 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
    11581 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    11641 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    11701 atcttaaagc ttctaggccg gccgatctcg gatctgacaa tgttcagtgc agagactcgg
    11761 ctacgcctcg tggactttga agttgaccaa caatgtttat tcttacctct aatagtcctc
    11821 tgtggcaagg tcaagattct gttagaagcc aatgaagaac ctggttgttc aataacattt
    11881 tgttcgtcta atatttcact accgcttgac gttggctgca cttcatgtac ctcatctata
    11941 aacgcttctt ctgtatcgct ctggacgtca tcttcactta cgtgatctga tatttcactg
    12001 tcagaatcct caccaacaag ctcgtcatcg ctttgcagaa gagcagagag gatatgctca
    12061 tcgtctaaag aactacccat tttattatat attagtcacg atatctataa caagaaaata
    12121 tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt atcgtatgag
    12181 ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac gcggtcgtta
    12241 tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga gctccaagcg
    12301 gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa
    12361 tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt aaaaaagatt
    12421 tgcgctttac tcgacctaaa ctttaaacac gtcatagaat cttcgtttga caaaaaccac
    12481 attgtggcca agctgtgtga cgcgacgcgc gctaaagaat ggcaaaccaa gtcgcgcgag
    12541 cgtcgactct agaggatccc cgggtaccga gctcgaattc gtaatcatgg tcatagctgt
    12601 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
    12661 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    12721 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    12781 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    12841 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggt
//
